Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal PROSIDING SEMINAR NASIONAL JURNAL LITBANG JURNAL KESEHATAN
Ratih Haribi
Unknown Affiliation
Education Health Professions

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
KONSENTRASI ALUMINIUM PADA IKAN ASAP YANG DIRENDAM DALAM LARUTAN TAWAS Haribi, Ratih; -, Yusrin
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2008: CONTINUING MEDICAL AND HEALTH EDUCATION (CMHE) | Peran Biomolekuler dalam Penegakan Diagnosis
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.506 KB)

Abstract

Ikan yang direndam larutan tawas 10% selama 1 jam, berkurang pahitdan bau amisnya, tanpa mengalami penurunan kadar proteinnya (Nurrahman, 2000).Tawas mengandung aluminium yang toksik dan dapat menyebabkanpenyakit akibat akumulasi yang melebihi batas ambang detoksifikasi tubuh (Darmono, 1996).Penelitian yang dilaksanakan bulan Mei – Nopember 2004, dilaboratorium Kimia Analisa dan Laboratorium Ilmu Pangan UniversitasMuhammadiyah Semarang (UNIMUS) ini bertujuan mengetahui konsentrasi aluminium yang terakumulasi daging ikan tongkol yang direndam pada larutan tawas dengan konsentrasi 4 %, 6 %, 8 %, 10 % dan 12 %, dengan lama perendaman 30 menit, 60 menit, 90 menit, 120 menit dan 150 menit. Pemeriksaan sampel dilakukan duplo dan diulang 3 kali. Analisis Aluminium dengan Metoda Thioglycolic Acid dan dibaca pada spectrofotometer dengan panjang gelombang (l) 510 nm,Analisis data dilakukan dengan Analisis of Varian Rancangan Acak KelompokHasilnya, konsentrasi ion aluminium per 10 gram daging ikan yangdirendam pada larutan tawas dengan konsentrasi yang berbeda dan waktu perendaman yang berbeda kemudian diasap, tidak ada beda nyata yaitu antara 0,266 – 0,408 ppm sedangkan daging ikan dengan perlakuan sama tanpa diasap, 0,266 – 0,413 ppm. Variasi konsentrasi tawas pada larutan perendam, variasi waktu perendaman dan pengasapan tidak mempengaruhi konsentrasi ion aluminium yang diakumulasi oleh daging ikan.Keywords : detoksifikasi, akumulasi.
ANALISIS PROTEIN PILLI Salmonella typhi lsolat RS. Kariadi Semarang DENGAN ELEKTROFORESIS SDS-PAGE Sri Darmawati; Ratih Haribi
JURNAL LITBANG Vol 2, No 3 (2005): Penelitian, Pengembangan, dan Pengabdian
Publisher : JURNAL LITBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.729 KB)

Abstract

Background : Salmonella typhi is bacteria which causes typhoid fever. Typhoid fever is severally serious, infectious decease and is endemic decease in Indonesia with relatively high frequency rate around 358-810/100.000 people each year This decease is usually spreading along with many kinds of decease which also have relatively high mortality rate, that is 1-5% of the sufferers (Punjabi, NH. , 2 004) . The infection process of bacteria into human body is signified with bacteria Cell adhering in mucosa intestinal surface. Pilli is structured by pilli protein consisting of Several sub units of pilli protein Objective: conducting pilli protein Salmonella typhi isolate Kariadi Hospital Semarang Analysis through electrophoreses SDS-PAGE, so that take amount of sub unit of pilli protein with each its molecule weight can be found out. Method : This research is conducted through three stages; firstly, bacteria cultivation with Biphasic media (BHI Agar and broth BHI ); secondly, pilli isolation with Ehara method (/956); Thirdly, sub unit of pilli protein separation with I 2% SDS-PAGE based on Lemmli method (1970). Result: There are four major sub units of protein in pilli, that is, 36kDa; 26,5 kDa; 22,2kDa; 18,6lrDq and there are also four minor sub units of protein pilli, that is, 116 kDa; 62,3kDa;45kDa;20,9kDa,andseveral other minor sub units of protein with every thin band. Keywords : Salmonella and Electrophoresis
KADAR HAEMOGLOBIN PADA BURUH WANITA YANG BEKERJA DI MALAM HARI Ratih Haribi
JURNAL LITBANG Vol 1, No 1 (2004): Penelitian, Pengembangan dan Pengabdian
Publisher : JURNAL LITBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (672.542 KB)

Abstract

From 30 samples, 19 female labours who have normal and 11 of them have under normal haemoglobine concentration. And than anolized in Chi-Square, so gaining the result reporting there's not significant corelation hetween work hours and haemoglohine concentration. The research samples refers to thefemal.e lobours wha have night work hoursrunge between 5 to l0 hours und more than ten hours.Concerning the extrafooding provided hy Factory, relate with haemoglohine concentration, i.e, l9 female labours who have normal haemoglohine cancentrution, who some of them eat up the extrofooding, 7 of them sometimes eat up antl 3 of them don't eat. Concerning the samples of 30 female labours, I I of them have abnormally haemoglobin concentotion, i.e, 3 female labours don't eut up the extrafooding and 9 don't eut them at all.In conclusion, the average of female lahours who work at night in PT X and who eat up the extrafooding having normal haemoglobine concentration. Meonwhile the female labours who don't eat them have undernormally haemoglobine concentration. The exffafooding provided by PT X is significant against the haemoglobine concentation offemale labours who work at night.Keywords : Haemoglobine, female labours and work at night
PEMERIKSAAN Escherichia coli PADA AIR BAK WUDHLU 10 MASJID DI KECAMATAN TLOGOSARI SEMARANG Ratih Haribi; Khoirul Yusron
JURNAL KESEHATAN Vol 3, No 1 (2010): Ilmu-Ilmu Kesehatan
Publisher : JURNAL KESEHATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (565.973 KB)

Abstract

Water tanks for wudhlu at the mosque in Tlogosari Semarang, comes from wells, rarely drained through pipes and used without a shower or faucet. Ablution basin water users, directly dipping the body into the tuband rinse with water from the tub ablution.Escherichia coli was the coliform group bacteria, which are used for indicators of water pollution by the stool, because the bacteria are normal flora of the colon, where stool in the process. Thus if the germs get onthe water, means water contaminated by stool. E. coli can cause diarrhea in its host, if high population immunity and depressed conditions.Based on the above, it is necessary to study the bacteriology of water washing basin, which aim to know the germ E. coli in it, so it can be given information about sanitation and the provision of proper ablutionwater for the community.When the study began in March 2008 to complete. Place of research conducted at the Laboratory of Microbiology, University of Muhammadiyah Semarang, Jl. Wonodri Sendang No. 2A Semarang.Samples were taken in total population at l0 mosques, on 10, 15 and March 20,2008, using a sterile bottle brown, henceforth conducted l00x dilution, were cultured in media Mac Concey (370C incubation, for 24hours, gram staining, biochemical tests, and repeat 2 timesExamination results show that in all there ablution basin E. coli and the population of most meetings occur at the mosque 2, 5 and 6 because of the distance ablution basin less than 2 feet from the septic tank. Insamples from the mosque 3 and 9 populations of E. coli meetings, because the distance was only 5 feet from septic tanks. Besides E. coli bacteria there are also other means such as Enterobacter sp., Pseudomonas sp, Citrobacter and Proteus vulgaris diwersus ablution basin Distance from septic tanks that are too close to allow the bacteria contamination stool into the washing tub through the water that seeps into the groundKey words: coliform, the host, normal flora
KELAINAN FUNGSI HATI DAN GINJAL TIKUS PUTIH (Rattus norvegicus, L.) AKIBAT SUPLEMENTASI TAWAS DALAM PAKAN Ratih Haribi; Sri Darmawati; Tri Hartiti
JURNAL KESEHATAN Vol 2, No 2 (2009): Ilmu-Ilmu Kesehatan
Publisher : JURNAL KESEHATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (809.171 KB)

Abstract

Abstract Alum is used to improve the quality of food containing toxic heavy metal ions which can interfere with Aluminum enzymatic system, and tissue damage. Liver and kidney are the most used network is affected, because it is a detoxification organ. Liver and kidney damage can be detected by an enzyme concentration of SGOT, SGPT, Billirubin, Protein, Ureum and Creatinin in the blood This study aims to find out the effects of alum in a feed supplement for liver and kidney damage in a clinic conducted from May to Oclober 2007, at the Laboratory of the University Clinic Patologt Muhammadiyah Semarang. Sample studies of white rats (Rattus norvegicus, L.), aged 2 months with weight average of 200 grams. 0o/o dose treatment (without supplementation), 0.05%, 0.1%, 0.2%, 0.5%, 1% and 0% (without supplementation), and subsequent treatment with a dose of 2%, 3%, 4%, 5 % and 6% volum, who every day put into the stomach of rats l0 mL Clinical laboratory tests performed at the time before treatment (control), 4 weeks, 6 weeks and 8 weeks of exposure time. Examination AST and ALT with Ultra Violet Test methods, Total Billirubin with modifications Groff Jendrasik method, total protein Colorimetri method, U Berthelot method, Creatinin Jaffe method. Clinical chemistry tests showed that supplementation influence of alum on the concentration of Enzymes and other factors in the blood of mice associated with damage to liver and kidney tissue. Level of organ damage significant with alum in a feed supplement. The higher the concentration of alum disuplementasion and the longer exposure time resulted in damage to the liver and kidneys getting worse.
Analisis Molekuler Profil Protein Pilli untuk Mengungkap Hubungan Similaritas 26 Strain Salmonella typhi Isolat Jawa Sri Darmawati; Ratih Haribi; Syaiful Anwar
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2012: SEMINAR NASIONAL HASIL PENELITIAN 2012
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.181 KB)

Abstract

Variasi dan hubungan similaritas 26 strain Salmonella typhi Isolat Jawa merupakanawal untuk melacak protein sub unit pilli spesifik yang memiliki aktivitas hemaglutinasi.Tujuan penelitian analisis molekuler profil protein pilli untuk mengungkap hubungansimilaritas 26 Strain S. typhi Isolat Jawa.Analisis dilakukan terhadap 26 strain yang berasal dari Surabaya, Madiun,Malang, Salatiga, Magelang, Bandung, Bogor, Jakarta, Yogyakarta dengan elektroforesisSDS-PAGE. Analisis hubungan similaritas digunakan program MVSP, untukmengkonstruksi dendogram yang mencerminkan klasifikasi dari 26 strain S. typhi IsolatJawa berdasarkan nilai indeks similaritas (S SM ) dengan algoritma UPGMA.Hasil analisis profil protein pili menunjukkan (1) Jumlah pita protein sub unitpilli bervariasi : 8-17 pita, BM tertinggi 200 kD, terendah 10 kD, dengan 20 karakter. (2)Protein 100 kD, 50 kD, 45 kD dan 40 kD adalah protein sub unit pilli yang dimiliki oleh26 strain S. typhi Isolat Jawa. (3) Dari 26 strain S. typhi Isolat Jawa terdiri dari duakelompok besar yang mempunyai indeks similaritas 61,2%. Kelompok pertama adalahstrain S. typhi Isolat Jawa Tengah dan Jawa Timur, dan kelompok ke dua adalah strain S.typhi Isolat Jawa Barat dan DKI. Pita 14 (12,5 kD) dan 15 (78kD) dari protein sub unitpilli hanya dimiliki strain S. typhi dari Surabaya. Pita 18(35kD) dan 20 (72kD) dariprotein sub unit pilli hanya dimiliki oleh Strain S. typhi dari DKI Jakarta.
Co-Authors Khoirul Yusron Laksono Trisnantoro Sri Darmawati TRI HARTITI Yusrin -