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All Journal PROSIDING SEMINAR NASIONAL JURNAL LITBANG JURNAL KESEHATAN
Ratih Haribi
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Journal : JURNAL LITBANG

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ANALISIS PROTEIN PILLI Salmonella typhi lsolat RS. Kariadi Semarang DENGAN ELEKTROFORESIS SDS-PAGE Sri Darmawati; Ratih Haribi
JURNAL LITBANG Vol 2, No 3 (2005): Penelitian, Pengembangan, dan Pengabdian
Publisher : JURNAL LITBANG

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Abstract

Background : Salmonella typhi is bacteria which causes typhoid fever. Typhoid fever is severally serious, infectious decease and is endemic decease in Indonesia with relatively high frequency rate around 358-810/100.000 people each year This decease is usually spreading along with many kinds of decease which also have relatively high mortality rate, that is 1-5% of the sufferers (Punjabi, NH. , 2 004) . The infection process of bacteria into human body is signified with bacteria Cell adhering in mucosa intestinal surface. Pilli is structured by pilli protein consisting of Several sub units of pilli protein Objective: conducting pilli protein Salmonella typhi isolate Kariadi Hospital Semarang Analysis through electrophoreses SDS-PAGE, so that take amount of sub unit of pilli protein with each its molecule weight can be found out. Method : This research is conducted through three stages; firstly, bacteria cultivation with Biphasic media (BHI Agar and broth BHI ); secondly, pilli isolation with Ehara method (/956); Thirdly, sub unit of pilli protein separation with I 2% SDS-PAGE based on Lemmli method (1970). Result: There are four major sub units of protein in pilli, that is, 36kDa; 26,5 kDa; 22,2kDa; 18,6lrDq and there are also four minor sub units of protein pilli, that is, 116 kDa; 62,3kDa;45kDa;20,9kDa,andseveral other minor sub units of protein with every thin band. Keywords : Salmonella and Electrophoresis
KADAR HAEMOGLOBIN PADA BURUH WANITA YANG BEKERJA DI MALAM HARI Ratih Haribi
JURNAL LITBANG Vol 1, No 1 (2004): Penelitian, Pengembangan dan Pengabdian
Publisher : JURNAL LITBANG

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Abstract

From 30 samples, 19 female labours who have normal and 11 of them have under normal haemoglobine concentration. And than anolized in Chi-Square, so gaining the result reporting there's not significant corelation hetween work hours and haemoglohine concentration. The research samples refers to thefemal.e lobours wha have night work hoursrunge between 5 to l0 hours und more than ten hours.Concerning the extrafooding provided hy Factory, relate with haemoglohine concentration, i.e, l9 female labours who have normal haemoglohine cancentrution, who some of them eat up the extrofooding, 7 of them sometimes eat up antl 3 of them don't eat. Concerning the samples of 30 female labours, I I of them have abnormally haemoglobin concentotion, i.e, 3 female labours don't eut up the extrafooding and 9 don't eut them at all.In conclusion, the average of female lahours who work at night in PT X and who eat up the extrafooding having normal haemoglobine concentration. Meonwhile the female labours who don't eat them have undernormally haemoglobine concentration. The exffafooding provided by PT X is significant against the haemoglobine concentation offemale labours who work at night.Keywords : Haemoglobine, female labours and work at night
Co-Authors Khoirul Yusron Laksono Trisnantoro Sri Darmawati TRI HARTITI Yusrin -