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All Journal Jurnal Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Kedokteran Hewan
Atik Ratnawati
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Veteriner

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Real Time Polymerase Chain Reaction : Perangkat Diagnostic Alternatif untuk Melacak Virus Nipah (REAL TIME POLYMERASE CHAIN REACTION : AN ALTERNATIVE DIAGNOSTIC TOOL TO DETECT NIPAH VIRUS) Indrawati Sendow; Atik Ratnawati; Raden Mas Abdul Adjid; Muharam Saepulloh
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.356 KB)

Abstract

Nipah is a dangerous zoonotic disease with a high social, economical and psychological impact. Fruitbat Pteropus sp. is one of the nipah virus  reservoir host. As the virus is categorized as a dangerous zoonoticdisease that cause fatal in human, all works related to live virus should be conducted in a laboratory withBSL4 facilities. The detection of nipah virus using real time PCR to replace virus isolastion can thereforebe conducted in a laboratory without BSL4 facilities. The results was further  confirmed at referencelaboratory at   Australian Animal Health Laboratory ( AAHL) Geelong, Australia, indicated that nipahvirus can be detected in saliva of fruit bat P. vampyrus in Medan North Sumatera.
Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton Asmarani Kusumawati; Atik Ratnawati; Ida Arlita Wulandari; Sri Hartati; Tri Untari
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA.
Pengembangan Kontrol Positif Sintetik dan Metode Gradient Reverse Trancriptase-Polymerase Chain Reaction (RT-PCR) Gen VP6 Bovine Rotavirus Group A Dyah Ayu Hewajuli; Pratama Y; Winarsongko A; Purwani A; Ajeng Fabeane; Suyatno T; Harimurti Nuradji; Nur Sabiq; Atik Ratnawati; Muharam Saepulloh; Ni Luh Putu Dharmayanti
Jurnal Veteriner Vol 24 No 2 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.2.187

Abstract

Rotavirus adalah jenis virus yang sering menyebabkan diare. Rotavirus grup A merupakan penyebab utama diare pada sapi. Rotavirus dibedakan menjadi delapan kelompok (A-H) berdasarkan perbedaan antigenik dan keragaman genetik protein VP6. Uji Gradient Reverse Trancriptase Polymerase Chain Reaction (RT-PCR) bersifat sensitif, spesifik, dan cepat untuk mendeteksi rotavirus grup A dalam sampel feses. Tujuan dari penelitian ini adalah mengembangkan kontrol positif sintetik dan mengoptimasi RT-PCR satu langkah dengan target gen VP6 untuk deteksi rotavirus grup A dari sampel feses. Kontrol positif sintetik bovine rotavirus grup A gen VP6 disintesis dengan gBlocks Gene Fragments. Optimasi menggunakan metode Gradient Reverse Trancriptase Polymerase Chain Reaction (RT-PCR). Hasil penelitian menunjukkan bahwa kontrol positif sintetik dan primer - menghasilkan pita jelas pada 1356 basepairs (bp) pada suhu annealing (56,4; 59,4; 61,6)ºC, sedangkan kontrol positif sintetik dan primer - menghasilkan pita jelas pada 450 bp terutama pada suhu annealing (45; 45,4; 46,4; 48,1; 50,3; 53,1;56,4; 59,4; 61,6)ºC. Selanjutnya, suhu annealing yang menghasilkan pitaoptimal digunakan dalam metode RT-PCR pada sampel penelitian. Hasil RT-PCR dari sampel penelitian menunjukkan 157 sampel negatif terhadap Rotavirus dengan primer Chinsangaram et al., 1993 tetapi satu sampel positif terhadap Rotavirus dengan primer Wang et al., 2019 yang ditandai dengan pita di 450 bp. Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwa pengembangan kontrol positif sintetis dan optimasi RT-PCR untuk deteksi Bovine Rotavirus grup A dengan gen target VP6 dapat digunakan sebagai metode skrining untuk mendeteksi Rotavirus pada sampel lapang.
Co-Authors Ajeng Fabeane Asmarani Kusumawati Dyah Ayu Hewajuli harimurti nuradji Ida Arlita Wulandari Indrawati Sendow Indrawati Sendow Indrawati Sendow Muharam Saepulloh Muharam Saepulloh Ni Luh Putu Dharmayanti NLP I Dharmayanti NLP Indi Dharmayanti Nur Sabiq Nur Sabiq Assadah Pratama Y Purwani A R.M. Abdul Adjid Raden Mas Abdul Adjid Sri Hartati Suyatno T Tri Untari Winarsongko A