This study aims to examine and determine the formulation of starch-chitosan and filler materials in bioplastic production in relation to the characteristics of the resulting bioplastic and the optimum conditions for bioplastic formulation, to obtain cellulose fibers from pineapple leaves with nanometer dimensions, and to obtain bioplastic from the application of nanoserats with mechanical and physical properties comparable to plastic in accordance with Indonesian National Standards (SNI). In this study, the starch-chitosan mass ratio used varied between 9:1, 8:2, and 7:3 (gram/gram), and the addition of corn stalk cellulose fiber filler varied between 0 grams, 0.5 grams, and 1 gram. The sieve size for starch and filler was 200 mesh. In the bioplastic manufacturing process, the glycerol concentration was set at 10% (weight percent), the stirring time was 35 minutes at a temperature of 75℃, and the stirring speed was 375 rpm. The cellulose fibers produced were still micro in size with a diameter of 1.078–1.224 µm. The results showed that the cassava peel starch chitosan bioplastic with pineapple leaf filler was close to the Indonesian National Standard (SNI) values. The analyses performed included mechanical properties (tensile strength, elongation percentage), physical properties (water absorption and biodegradation test, water vapor permeability), and functional group analysis using FTIR. This bioplastic has the best tensile strength value of 21.74 MPa, elongation of 105.70%, water absorption of 150%, biodegradation of 106.25%, and water vapor permeability of 2.62 g/m².