Rini Budi Hastuti
Universitas Diponegoro

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Improving Silvofishery Management Through Seedling Growth Environment Quality Dynamic Relation Analysis Hastuti, Endah Dwi; Hastuti, Rini Budi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10490

Abstract

Mangrove plants are sensitive to environment condition. This research aimed to analyze the linkages of mangrove growth and environment dynamics and to estimate the growth of mangrove along with the environment dynamics. The research was conducted through the field experiment by the plantation of A. marina in silvofishery pond canals. Data collection was conducted for 18 months with 3 months observation interval. The environment variables observed including temperature, salinity, turbidity, pH, dissolved oxygen, TSS, sediment organic matter, nitrogen and phosphorus, and the growth of mangrove seedling. Analysis was conducted through regression and modelling with Powersim software. The result showed that the height growth was affected by dissolved oxygen, temperature, salinity, turbidity and pH, while the diameter growth was affected by TSS concentration. Inversely, the growth of mangrove also had a significant effect on temperature, change of organic matter and nutrient sediment concentration. Simulation showed that the height and diameter growth rates of A. marina seedling were dynamically changed among periods. Simulated seedling growth for one year forecast resulted in the rate of 0.115024 to 0.282294 cm/day for height and 0.001287 to 0.006031 cm/day for diameter. The simulation also indicated the continuous accumulation of organic matter and nitrogen over time. This research concluded that under limited environment dynamic, the growth of mangrove could be estimated. This model is a novelty in ecological studies. This research might initiates the more advanced ecological studies. Systematic estimation of ecosystem behaviour could be applied to formulate the best management practices, particularly in the silvofishery activities.
Isolasi dan Identifikasi Jamur Indigenous Rhizosfer Tanaman Kentang dari Lahan Pertanian Kentang Organik di Desa Pakis, Magelang Purwantisari, Susiana -; Hastuti, Rini Budi
Bioma : Berkala Ilmiah Biologi Vol. 11, No. 2, Tahun 2009
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3261.717 KB) | DOI: 10.14710/bioma.11.2.45-53

Abstract

Jamur rhizosfer merupakan salah satu faktor biotik yang dapat menginduksi ketahanan tanaman terhadappenyakit. Jenis tanah yang mengandung mineral organik dan anorganik mempengaruhi jenis jamur yang ada. Jamuryang ada di rhizosfer dapat melindungi tanaman terhadap patogen dan meningkatkan kesuburan pertumbuhantanaman sehinggga digolongkan sebagai jamur pemacu kesuburan tanaman (biofertilizer). Dengan demikian isolatjamur yang diisolasi dari rhizosfer tanaman sehat berpeluang besar menjadi alternatif penting bahan bakubiofertilizer tanaman. Penelitian ini bertujuan untuk mengetahui genus-genus jamur tanah indigenous di lahanpertanian kentang organik di Desa Pakis Magelang. Isolasi jamur menggunakan metode pengenceran berseri (SerialDilution Method ) hingga 10-5 pada medium PDA (Potato Dextrose Agar). Isolat jamur yang didapatkandiidentifikasi secara makroskopis dan mikroskopis menggunakan buku identifikasi Domsch, et al., (1980). Dari hasilisolasi diperoleh 8 (delapan ) isolat jamur yang termasuk ke dalam genus Trichoderma (2 isolat), Penicillium (1isolat), Phytophthora (2 isolat), Mucor (1 isolat) dan 2 isolat jamur yang belum teridentifikasi sehingga belumdiketahui genusnya.
Pengaruh Perbedaan Konsentrasi Pupuk Organik Cair tehadap Produksi Bawang Merah (Allium ascalonicum L) Setiyowati, Setiyowati; Haryanti, Sri; Hastuti, Rini Budi
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 2, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.533 KB) | DOI: 10.14710/bioma.12.2.44-48

Abstract

Shallot is one an important of vegetable comodity, both in economic value and nutrition ingredients. One of an effort to support the increasing of shallot yield with plantation technology by giving organic liquid fertilizer. The experiment we studied to find out the effect of organic liquid fertilizer with different consentration on yield of shallot. The experiment used Completely Randomized Design consisted 6 consentration of organic liquid fertilizer 0 ml/L (P0), 1 ml/L (P1), 2 ml/L (P2), 3 ml/L (P3), 4 ml/L (P4), 5 ml/L (P5) with 4 replication. The results show that consentration of 4 ml/L organic liquid fertilizer is increas the number of bulb and consentratin of 5 ml/L is increas in tuber wet weight.
Pengaruh Perasan Sargassum crassifolium dengan Konsentrasi yang Berbeda terhadap Pertumbuhan Tanaman Kedelai (Glycine max (L) Merill) Kusumaningrum, Indri; Hastuti, Rini Budi; Haryanti, Sri
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 15, No 2 (2007): Volume XV, Nomor 2, Oktober 2007
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (39.672 KB) | DOI: 10.14710/baf.v15i2.2567

Abstract

Soybean is one important vegetable in Indonesia, soybean consist of 35 gram of protein for every 100 gram. Even more upon consist of the superior variety. Contains soybean protein could reach 40 – 43% to interest the growth of soybean plants. We can complete various methods, one of them is the use of see weed, because it consist of high phytohormone. Sargassum crassifolium is one see weed of phaephy Cese Class that is potential to accelerate growth of plants from other species. Compounds consisted inside are auxin, giberelin, cytolaninand other mineral etc. The purpose of the research is to acknowledge the effect of Sargassum crassifolium extract with different concentration toward the growth of soybean plants and acknowledge the extract concentration that effect to the growth of soybean plants. This research was executed in October – November 2001 in Green House and the Laboratory of Structure and Plant function Biology F.MIPA UNDIP. The research lay out used was the extract of Sargassum crassifolium with concentration of 25%, 50%, 75%, 100% and without extract ( 0% ). Each treatment was repeated for 5 time. The gained data was analyzed with variety investigation analyzed were real difference level of 5%. The research result shows that the extract of Sargassum crassifolium effects to plant heigh, but it does not effect to number of leaf, wet weight and dry weight soybean plant. The plant height in creases upon the extrct concentration of Sargassum crassifolium of 50%.
PENGARUH KOMPOS DENGAN STIMULATOR EM 4 (EFFECTIVE MICROORGANISMS 4) TERHADAP PERTUMBUHAN DAN PRODUKSI JAGUNG MANIS (Zea mays var, Saccharata) Roihanna, Nur; Haryanti, Sri; Hastuti, Rini Budi
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 2 (2009): Volume XVII, Nomor 2, Oktober 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.202 KB) | DOI: 10.14710/baf.v17i2.2564

Abstract

Production of sweet com, which is rich of carbohydrate, but less of protein and fat, still hasn’t fulfilled market needs. The increase of sweet com production may be carried out by improving the growth and production of this plant byproviding required hara element supply. This supply may be implemented by giving compost. The making of compost requires long time; it therefore should be added with stimulator EM4, namely liquid culture of microorganism like Lactobacillus, Actinomycetes and photosynthesis bacteria which may acceleratethe composing process. The problem formulation of this research is whether compost with EM4 stimulator influences the growth and producyion of sweet com and how many doze of compost with EM4 stimulator which may give the best result. The aim of the research is to evaluate the effect of compost with EM4stimulator against the growth and production of sweet com and also to evaluate the best doze of compost with EM4 stimulator which may gives the highest result. The research uses Complete Random Design with 4 treatments in which each treatment is repated 5 times. Those treatments are PO (control), P1 (compost 47,1 g/polybag), P2 (compost 94,2 g/polybag), P3 (compost 141,3 g/polybag). The obtained data was analyzed using ANOVA and BNT test when there is a significant difference.The result of the research indicates that compost with EM4 stimulator may increase the growth and production of sweet com and the doze of compost giving the highest result in the research is 141,3 g/polybag.
Pengaruh Lama Penyimpanan Terhadap Kadar Gula dan Vitamin C pada Buah Jeruk Siam ( Citrus nobilis var. microcarpa ) Helmiyesi, Helmiyesi; Hastuti, Rini Budi; Prihastanti, Erma
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 16, No 2 (2008): Vol. XVI, No. 2, Oktober 2008
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.747 KB) | DOI: 10.14710/baf.v16i2.2620

Abstract

The aim of the research is to find out the effect of long storage against glucose and vitamin C degree andalso to find out how many days of the storage whioch can still maintain the glucose and vitamin C degreeupon siam orange ( Citrus nobilis var. microcarpa ). It is expected that the research may give anyinformation especially for the seller of siam orange about the effect of long storage against glucose andvitamin C degree and how many days the storage should be performed. The experimet includes 4 treatmentsand 5 repetilions. The treatment is the length of the storage, namely 0 day ( control ), 5 days, 10 days and 15days. The observed parameters are glucose, vitamin C degree and fruit weight decrease. The research usescomplete Random design, when there is a real difference Duncan test will be used upon significant degree of5 %. The result of the research indicates that the glucose degree of siam orange upon the storage of 5 and 10days increases compared to the control. Upon the storage of        15 days the glucose degree decreases comparedto the storage of 5 and upon the storage of 5 days does not change compared to the control, but it startsdecreasing upon the storage of 10 and 15 days.
Kapasitas Penyerapan dan Penyimpanan Air pada Berbagai Ukuran Potongan Rumput Laut Sargassum sp sebagai Bahan Pupuk Organik Haryza, Yuvita Christovora; Hastuti, Rini Budi
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 1 (2009): Volume XVII, Nomor 1, Maret 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.935 KB) | DOI: 10.14710/baf.v17i1.2533

Abstract

Brown algae Sargassum sp is one species of seaweed that is existed in Indonesia. The species consist of kalium, macro and micro mineral, and gel with the existence of content, Sargassum sp can be managed as the organic fertilizer to increase the huminity of the fertilizer that is able to support the water absorbtion by the plant so that it can optimize the plant growth. This research possesses purpose to acknowledge the capacity amount of Sargassum sp toward the absorbtion and storage process of the water with the different slice measure that is 1 cm; 0,5cm; 0,25 cm. This research was held in the month of April – May 2006 in Laboratory of Structure Biology andPlant Function of MIPA Fakulty of Diponegoro University. The gained result from the research is the existence of the influence of the slice of Sargassum sp toward the absorbtion and storageprocess of water. The slice of 0,25 cm is the slice that possesses the biggest ability of the storage process of water whereas the slice of 0,5 cm is the slice that possesses the longest ability of the storage process of water.
Adaptasi Morfologi Fisiologi dan Anatomi Eceng Gondok (Eichhornia crassipes (Mart) Solm) di Berbagai Perairan Tercemar Haryanti, Sri; Hastuti, Rini Budi; Hastuti, Endah Dwi; Nurchayati, Yulita
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 14, No 2 (2006): Vol. XIV, No. 2, Oktober 2006
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.688 KB) | DOI: 10.14710/baf.v14i2.2576

Abstract

The research was conducted to determine the morphology , physiology and anatomy adaptation from eceng gondok in waste water pollution. The research was done at the laboratory of Botany Structure and function. The design of the experiment use was split plot design based of Completely Randomized Design. First factor was density 2 and 4, second factor was 3 waste was LIK, drugs and metal foundry with water to control. The treatment was 3 replicates, The experiment was long 20 days with leaf morphology parameter ,growth/ physiology parameter ( long root, content of chlorophyl leaf) and anatomy parameter ( leaf,and stalk leaf and root). The result of the research indicated was leaf morphology adaptation on drugs waste and physiology adaptation on third waste and root anatomy adaptation was drugs waste with protrution branch root.
PROFIL TAMPILAN FISIK DAN KANDUNGAN KURKUMINOID DARI SIMPLISIA TEMULAWAK (CURCUMA XANTHORRHIZA ROXB) PADA BEBERAPA METODE PENGERINGAN Zahro, Laely; Cahyono, Bambang; Hastuti, Rini Budi
JURNAL SAINS DAN MATEMATIKA Volume 17 Issue 1 Year 2009
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8075.172 KB)

Abstract

ABSTRAK-Penelitian dilakukan untuk mempelajari pengaruh beberapa metode  terhadap kualitas  simplisia temulawak yakni dengan melihat tampilan fisik dan kandungan kurkuminoid temulawak. Rimpang temulawak segar dibersihkan, dipotong, dikeringkan dengan beberapa metode  pengeringan yaitu pengeringan oven dengan suhu 60oC, pengeringan dengan oven lampu suhu 30oC, pengeringan dengan sinar matahari dari jam 08.00-11.00 dan dari jam 08.00-15.00, dihaluskan, diekstraksi, dan dianalisis menggunakan spektrofotometer UV- visible. Simplisia hasil pengeringan oven dan oven lampu mempunyai wama yang cerah dan permukaannya berwarna  jingga kekuningan sedangkan simplisia hasil pengeringan matahari berwarna gelap dan terinfeksi jamur  putih. Hasil kandungan kurkuminoid ditampilkan dalam persen dari berat kering dan diuji dengan Analisis Varian Eka Arah Kruskal Wallis pada taraf signifikansi 5%. Kadar kurkuminoid yang dihasilkan dari pengeringan oven yaitu 0,68%, 0,92%, 0,91%, dan 0,82%, dari pengeringan oven lampu yaitu 0,71%, 0,83%, 0,57%, dan 0,97%, dari pengeringan matahari pada jam 08.00-11.00 yaitu 0,82%, 0,99%, 0,64%, dan 0,89% dan dari pengeringan matahari pada jam 08.00-15.00 yaitu 0,80%, 0,89%, 0,84%, dan 0,93%. Kadar kurkuminoid yang diteliti dari beberapa metode pengeringan tersebut menunjukkan  tidak ada perbedaan yang sighnifikan. Kata  kunci : kurkuminoid, pengeringan, simplisia,  uv-visibel
Effects of Light for Callus Induction of Mangrove Plant (Rhizophora Apiculata Bi) by In Vitro I’anatushshoimah, I’anatushshoimah; Nurchayati, Yulita; Prihastanti, Erma; Hastuti, Rini Budi
Life Science Vol 9 No 2 (2020): November 2020
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v9i2.47157

Abstract

Initiation of Rhizophora apiculata BI propagation in vitro can be done by callus culture. Induction of mangrove callus has the problem of browning emergence. The phenomenon of browning can be overcome by limiting the treatment of light. The purpose of this research were to study the effect of light duration on callus growth, to understand the duration of light treatment that can spur the optimal callus growth, and to understand the effect of the light treatment on the browning event. The explants used were leaf grown in Murashige and Skoog (MS) medium with addition of NAA 1 ppm, BAP 0.3 ppm and activated charcoal 12 g / l. The treatments included 24 hour dark treatment, 24 hours light, dark 16 hours light 8 hours, light 8 hours dark 16 hours with 4 repetitions each. The results showed that the light treatment could induce callus formation while 24 hour dark treatment could reduce browning. All explant grown in conditions exposed to light and dark spewn exudate. The best callus growth (0.1939 g) was obtained in the T16G8 treatment (light 16 hours, dark 8 hours) with the time of the emergence of callus 6 DAP. Research about tissue culture with mangrove plants is rarely conducted because of high browning possibility. Thus, the novelty of this research lies upon the process of browning prevention using light duration treatment so browning could be prevented and mangrove culture could produce callus. Inisiasi perbanyakan Rhizophora apiculata BI secara in vitro dapat dilakukan dengan kultur kalus. Induksi kalus mangrove memiliki masalah munculnya kecoklatan. Fenomena pencoklatan bisa diatasi dengan membatasi perawatan cahaya. Tujuan dari penelitian ini adalah untuk mempelajari pengaruh lama pencahayaan terhadap pertumbuhan kalus, mengetahui lama perlakuan cahaya yang dapat memacu pertumbuhan kalus yang optimal, dan mengetahui pengaruh perlakuan cahaya terhadap kejadian pencoklatan. Eksplan yang digunakan adalah daun yang ditanam pada media Murashige dan Skoog (MS) dengan penambahan NAA 1 ppm, BAP 0,3 ppm dan arang aktif 12 g / l. Perlakuan tersebut meliputi perlakuan gelap 24 jam, terang 24 jam, terang gelap 16 jam 8 jam, terang 8 jam gelap 16 jam dengan masing-masing 4 pengulangan. Hasil penelitian menunjukkan bahwa perlakuan cahaya dapat menginduksi pembentukan kalus sedangkan perlakuan gelap 24 jam dapat mengurangi pencoklatan. Semua eksplan tumbuh dalam kondisi terpapar eksudat yang dimuntahkan terang dan gelap. Pertumbuhan kalus terbaik (0,1939 g) diperoleh pada perlakuan T16G8 (terang 16 jam, gelap 8 jam) dengan waktu munculnya kalus 6 HST. Penelitian tentang kultur jaringan dengan tanaman mangrove jarang dilakukan karena kemungkinan kecoklatannya tinggi. Dengan demikian, kebaruan dari penelitian ini terletak pada proses pencegahan pencoklatan menggunakan perlakuan durasi yang ringan sehingga pencoklatan dapat dicegah dan kultur mangrove dapat menghasilkan kalus.