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All Journal Jurnal Ilmu Dasar Jurnal Biologi Tropis Makara Journal of Science
AERMA HASTUTY
1Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Bogor Km 46, Cibinong 16911, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Immunology & microbiology Mathematics

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Antibacterial and Anti-Biofilm Activities of Culture Filtrates from Schizophyllum commune, Coniothyrium sp., and Fusarium sp. Hastuty, Aerma; Mairani, Rahma; Rosada, Keukeu Kaniawati
Makara Journal of Science Vol. 24, No. 2
Publisher : UI Scholars Hub

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Abstract

Bacterial infections are usually caused by biofilms that are resistant to extreme conditions. Studies have shown that fungal extracts have the potential to inhibit biofilm formation. The aim of this study was to examine the activity of several fungal culture filtrates in inhibiting bacterial growth and biofilm formation. The fungi were identified by molecular method, and the effects of different concentrations (45%, 75%, and 90%), medium pH (6, 7, and 9), temperatures (30 and 37 °C), and incubation times (24, 48, and 72 h) of the filtrates on their anti-biofilm formation properties were investigated. Anti-biofilm assay was performed using the diffusion test and microtiter assay. The parameters examined included the diameter of the inhibition zone of each well and the optical density of the filtrate solution. Molecular identification based on ITS rDNA regions showed that the fungal isolates in this study were Schizophyllum commune (strain JSB2), Coniothyrium sp. (strain JB1-3), and Fusarium sp. (strain JBB2). Antibacterial assay showed that, among the culture filtrates obtained from all fungi, that from Fusarium sp. strain JBB2 exhibits the highest inhibitory activity. Biofilm inhibition assay further revealed optimum fungal culture filtrate activities at pH 7.
Characterization of Protease from Endophytic Fungi Fusarium sp. JE-DP4a Hastuty, Aerma; Herawati, Irma; Pikoli, Megga Ratnasari
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.27293

Abstract

Protease is a catalytic enzyme that catalyzes a proteolysis process and breaks down a protein into smaller polypeptides or amino acids. Endophytic fungi can produce bioactive compounds, one of which is protease enzymes. The papaya leaf endophytic fungi used in this study was Fusarium sp. strain JE-DP4a. This study was conducted to determine the characterization of protease from endophytic fungi Fusarium sp. strain JE-DP4a with variations in incubation temperature of 30, 37, and 44oC and pH variation of 5.5-8. Measurement of protease enzyme activity using a spectrophotometer UV-Vis at 280 nm. Based on the research results, the highest protease activity produced at a temperature of 44oC is 53.78 U/mL, and specific activity is 17.31 U/mg protein. Protease activity at pH 7 shows the highest was 46.72 U/mL.Thus it is ably concluded that the protease produced by Fusarium sp. strain JE-DP4a belongs to the neutral protease group.
Potential of Lactic Acid Bacteria Isolated from Fermented Tempe Food in Producing Fibrinolytic Enzymes Amanda, Nifsa Riski; Hastuty, Aerma; Putri, Dwi Hilda; Sulistiani, Sulistiani
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9554

Abstract

Cardiovascular disease is the leading cause of death worldwide, mainly due to blood vessel blockage caused by thrombus. Fibrinolytic enzymes are used to dissolve thrombus but are often limited by high production costs, low specificity, allergic reactions, and bleeding risks. As a safer alternative, bacteria from fermented foods offer several advantages, including low toxicity, cost-effective production, and high fibrin specificity, which reduces the risk of bleeding. This study investigated the potential and characteristics of fibrinolytic enzyme activity produced by lactic acid bacteria (LAB) isolated from tempeh. The fibrin plate method was used to identify LAB producing the enzyme, while enzyme activity was measured using the method of Varol et al. (2023), and protein concentration was analyzed using a modified Bradford test. Thirteen LAB isolates showed fibrinolytic activity, with isolate PG01-2B selected as the most promising. The highest enzyme production occurred on the second day, reaching 83.41 U/mL. Characterization showed activity with acetic acid buffer pH 6 (102.18 U/mL) and at 75 minutes incubation time (99 U/mL). These results indicate that LAB from tempeh has strong potential as a source of fibrinolytic enzymes and can function as an alternative therapeutic agent for thrombus-related cardiovascular diseases.
Co-Authors Amanda, Nifsa Riski Dwi Hilda Putri herawati, irma Keukeu Kaniawati Rosada Mairani, Rahma Megga Ratnasari Pikoli Sulistiani Sulistiani, Sulistiani