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All Journal Jurnal Ilmu Dasar AL KAUNIYAH JURNAL BIOLOGI INDONESIA
Megga Ratnasari Pikoli, Megga Ratnasari
Prodi Biologi, Fakultas Sains dan Teknologi, UIN Syarif Hidayatullah Jakarta
Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Materials Science & Nanotechnology Mathematics

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Seleksi Konsorsium Mikroorganisme untuk Pembenah Lumpur Sidoarjo sebagai Media Tanam Pikoli, Megga Ratnasari; Setyawati, Adi Puji; Larasati, Tri Retno Dyah; Mulyana, Nana
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.82 KB) | DOI: 10.14203/jbi.v14i1.3660

Abstract

ABSTRACTThe mud that erupted in Sidoarjo, East Java, has been known from several studies can be used as plant growth media. However, its ability has limitations, particularly due to the very high heavy metal content and phenol will gradually poison the plants. Therefore, a study has been conducted in order to obtain a consortium of microorganisms which have ability to support plants growth and remediate the mud so that further can be used as a growth media. The selected microorganisms consist of Azotobacter sp. KDB2, Bacillus sp. KLBN1, Bacillus sp. BMC4, Pseudomonas sp. BMC6, Trichoderma sp. PJF6 and Trichoderma sp. PJF7F7, which were combined in five consortia, namely K1, K2, K3, K4, and K5. The selection was performed on the ability to produce indole acetic acid, dissolve phosphate, decrease phenol concentration, and decrease the concentration of Pb and Cd ion metals. The results showed that the five consortia have capacity in the five parameters examined. The ability to remove Pb by microbial consortia which consist of Azotobacter, Bacillus, Pseudomonas, and Trichoderma is a new information of the role of microorganism on heavy metal removal. The existence of positive and negative interactions within each consortium lead to differences in the kind of superior consortium generated from each parameter. The consortium K4 showed advantages in 3 parameters, that were in the indole acetic acid production and the Pb and Cd decreases, while the other consortia showed the best capacity only on one of the parameter examined. Therefore, the selected consortium recommended for fixing the Sidoarjo mud is K4, which consists of Azotobacter sp., Bacillus sp. BMC4, Pseudomonas sp. BMC6, Trichoderma sp. PJF6 dan Trichoderma sp. PJF7.Keywords: biofertilizer, bioremediation, Azotobacter, Bacillus, Pseudomonas, Trichoderma
Pemanfaatan Urea sebagai Sumber Nitrogen pada Biosolubisasi Batubara oleh Trichoderma sp. Mulyawati, Novi; Pikoli, Megga Ratnasari; Sugoro, Irawan; Aditiawati, Pingkan
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.566 KB) | DOI: 10.15408/al-kauniyah.v6i2.2824

Abstract

Lignite coal was found abundant in Indonesia, but usage for this type of coal was still relatively low. Economic value of coal increases when it is solubilized. Biosolubilization of coal by utilize of microbes produces compounds equivalent to petroleum. In this research, effect of urea on lignite biosolubilization by Trichoderma sp. was examined. Method of this research consisted of spore inoculum preparation, biosolubilization lignite coal with a variety of treatment that consists of treatment A (MSS + sucrose  1% + coal 5% + urea), and treatment B (MSS + sucrose  1% + coal 5%). Results showed that the addition of urea supported lignit coal biosolubilization by Trichoderma sp. based on increase in medium pH, concentration of phenolic and conjugated aromatic compounds, and activity of extracellular enzyme. In addition, result of product characterization using GCMS revealed compounds equivalent to 13,60%, 26,20% and 90,8% respectively for gasoline, kerosene and diesel components. Those confirmed that urea can be used as an alternative nitrogen source to support Trichoderma sp. in lignit biosolubilization producing petroleum compounds.
SELEKSI KONSORSIUM MIKROORGANISME UNTUK PEMBENAH LUMPUR SIDOARJO SEBAGAI MEDIA TANAM Pikoli, Megga Ratnasari; Setyawati, Adi Puji; Larasati, Tri Retno Dyah; Mulyana, Nana
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3660

Abstract

ABSTRACTThe mud that erupted in Sidoarjo, East Java, has been known from several studies can be used as plant growth media. However, its ability has limitations, particularly due to the very high heavy metal content and phenol will gradually poison the plants. Therefore, a study has been conducted in order to obtain a consortium of microorganisms which have ability to support plants growth and remediate the mud so that further can be used as a growth media. The selected microorganisms consist of Azotobacter sp. KDB2, Bacillus sp. KLBN1, Bacillus sp. BMC4, Pseudomonas sp. BMC6, Trichoderma sp. PJF6 and Trichoderma sp. PJF7F7, which were combined in five consortia, namely K1, K2, K3, K4, and K5. The selection was performed on the ability to produce indole acetic acid, dissolve phosphate, decrease phenol concentration, and decrease the concentration of Pb and Cd ion metals. The results showed that the five consortia have capacity in the five parameters examined. The ability to remove Pb by microbial consortia which consist of Azotobacter, Bacillus, Pseudomonas, and Trichoderma is a new information of the role of microorganism on heavy metal removal. The existence of positive and negative interactions within each consortium lead to differences in the kind of superior consortium generated from each parameter. The consortium K4 showed advantages in 3 parameters, that were in the indole acetic acid production and the Pb and Cd decreases, while the other consortia showed the best capacity only on one of the parameter examined. Therefore, the selected consortium recommended for fixing the Sidoarjo mud is K4, which consists of Azotobacter sp., Bacillus sp. BMC4, Pseudomonas sp. BMC6, Trichoderma sp. PJF6 dan Trichoderma sp. PJF7.Keywords: biofertilizer, bioremediation, Azotobacter, Bacillus, Pseudomonas, Trichoderma
Characterization of Protease from Endophytic Fungi Fusarium sp. JE-DP4a Hastuty, Aerma; Herawati, Irma; Pikoli, Megga Ratnasari
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.27293

Abstract

Protease is a catalytic enzyme that catalyzes a proteolysis process and breaks down a protein into smaller polypeptides or amino acids. Endophytic fungi can produce bioactive compounds, one of which is protease enzymes. The papaya leaf endophytic fungi used in this study was Fusarium sp. strain JE-DP4a. This study was conducted to determine the characterization of protease from endophytic fungi Fusarium sp. strain JE-DP4a with variations in incubation temperature of 30, 37, and 44oC and pH variation of 5.5-8. Measurement of protease enzyme activity using a spectrophotometer UV-Vis at 280 nm. Based on the research results, the highest protease activity produced at a temperature of 44oC is 53.78 U/mL, and specific activity is 17.31 U/mg protein. Protease activity at pH 7 shows the highest was 46.72 U/mL.Thus it is ably concluded that the protease produced by Fusarium sp. strain JE-DP4a belongs to the neutral protease group.
Co-Authors AERMA HASTUTY herawati, irma Irawan Sugoro Nana Mulyana Novi Mulyawati, Novi PINGKAN ADITIAWATI Setyawati, Adi Puji Setyawati, Adi Puji Tri Retno Dyah Larasati, Tri Retno Dyah