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All Journal Biotropika BERITA BIOLOGI Meditory : The Journal of Medical Laboratory
Yulianto Ade Prasetya
STIKES Rumah Sakit Anwar Medika Sidoarjo
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Aktivitas Antibakteri Nanoemulsi Minyak Lengkuas (Alpinia galanga L. Willd) dalam menghambat pertumbuhan Helicobacter pylori Yulianto Ade Prasetya; Khoirun Nisyak; Eviomitta Rizki Amanda
Biotropika: Journal of Tropical Biology Vol 7, No 3 (2019)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2019.007.03.7

Abstract

Helicobacter pylori termasuk patogen opportunistik yang memiliki ciri berupa Gram negatif, flagela lopotrikus, tidak berkapsul, dan urease positif. Bakteri ini mampu menyebabkan gastritis, tukak lambung, dan kanker lambung. Penelitian ini bertujuan untuk mengetahui aktivitas nanoemulsi minyak lengkuas (Alpinia galanga L. Willd) dalam menghambat pertumbuhan bakteri Helicobacter pylori. Nanoemulsi minyak lengkuas dibuat dengan bantuan magnetic stirer dan ultrasonikator kemudian dianalisa ukurannya dengan Particle Size Analyzer. Uji aktivitas terhadap H. pylori dilakukan dengan metode difusi sumuran. Hasil penelitian menunjukkan bahwa nanoemulsi minyak lengkuas 1% (ukuran droplet 19.9 nm) mampu menghambat bakteri H.pylori dengan zona hambat sebesar 9,5 mm. Nanoemulsi minyak lengkuas dapat digunakan sebagai kandidat pengobatan infeksi lambung sehingga mampu mencegah karsinoma lambung.
DETEKSI FENOTIPIK Methicillin Resistant Staphylococcus aureus (MRSA) PADA SAMPEL MAKANAN DI SIDOARJO Yulianto Ade Prasetya
Meditory : The Journal of Medical Laboratory Vol 7, No 1 (2019): Meditory, volume 7 , nomer 1 , Juni 2019
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.331 KB) | DOI: 10.33992/m.v7i1.554

Abstract

Methicillin-resistant Staphyloccocus aureus (MRSA) is an isolate that is resistant to the antibiotic methicillin and beta lactam group. The incidence of MRSA associated with nosocomial infections in various parts of the world is very high, but research on its spread in community infections is rarely reported. This study aims to detect the presence of phenotypic MRSA in food samples in Sidoarjo. The food samples (cilok, fried foods and tempura) collected were then weighed, diluted, and cultured in a selective medium and differential namely Manitol Salt agar. The yellow-colored colonies were then continued with microscopic testing and biochemical tests to distinguish between Staphylococcus species. Thirty eight collected Staphyloccus aureus isolates were then screened using Oxacillin 1 µg and there were eight (8) isolates that were positive for MRSA according to the criteria of the Clinical Laboratory Standart Institutre (CLSI). Eight of the isolates were tested for antibiotic sensitivity with the Kirby-Bauer method with Chloramphenicol 30 µg and Cotrimoxazole 25 µg. Eight MRSA (21%) isolates were resistant to Chloramphenicol and only four isolates were resistant to Cotrimoxazole. The presence of MRSA isolates in community infections needs to be watched out for considering these genes can be transmitted and spread between bacterial species
FORMULASI JAGUNG MANIS SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN BAKTERI PATOGEN Yulianto Ade Prasetya
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1574

Abstract

Background: Sources of carbohydrates and proteins to support bacterial growth are available abundance in nature with very affordable prices, including sweet corn. Aims: This research to determine growth of pathogenic bacteria Escherichia coli and Staphylococcus aureus by using sweet corn as an alternative medium with different formulations. Method: The method used was total plate count (TPC) with sweet corn variations used were 1g, 2g, 3g, 4g, and 5g and colony forming units (CFU) were calculated after being incubated for 24 hours at 37C. Result: The result showed that E. coli and S. aureus was able to grow optimally in the variation of 5g with a growth of respectively 46,7 x 107 CFU/mL and 112,7 x 107 CFU/ mL. This research is expected to be used as an alternative medium for routine microbial growth for the purpose of detecting contamination in human biological samples as well as for food and beverage products. Keywords: Sweet corn, Alternative medium, Escherichia coli, Staphylococcus aureus
AKTIVITAS NANOEMULSI MINYAK AGARWOOD BOUYA (Aquilaria agallocha) TERHADAP BAKTERI MULTIRESISTEN ANTIBIOTIK DAN NON-RESISTEN Yulianto Ade Prasetya
BERITA BIOLOGI Vol 21, No 1 (2022)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i1.4090

Abstract

Multiresistant bacteria are responsible for increasing morbidity, mortality, and health care costs. Multiresistant bacteria such as Methicillin Resistant Staphylococcus aureus (MRSA), Escherichia coli producing Extended Spectrum Beta Lactamases (ESBLs), and Klebsiella pneuomoniae producing ESBLS are resistant to more than three classes of antibiotics so that alternative natural-based treatments are needed to prevent the spread of resistance. This research aims to determine the activity of Agarwood bouya (Aquilaria agallocha) oil nanoemulsion against multiresistance and non-resistance antibiotic. Nanoemulsion is made by magnetic stirrer and sonicator. The variations of Agarwood Bouya oil used are 1%, 5%, 10%, 15%, and 20%. The size of the nanoemulsion formed was analyzed using a Particle Size Analyzer (PSA), while its clarity was measured by a UV-VIS spectrophotometer. Agarwood oil nanoemulsion activity test was carried out by disk diffusion method. The results showed that agarwood oil nanoemulsion with a concentration of 1% had the smallest size is 17.7 nm with a percent transmittance of 99.35%. The agarwood bouya oil nanoemulsion 20% was only able to inhibit ESBLs-producing E. coli by 3.3 mm and was included in the weak category. The non-resistant E. coli bacteria were able to actively inhibit with the inhibition zone at 13.3mm.
Co-Authors Eviomitta Rizki Amanda Khoirun Nisyak