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Lies Parede Hernomoadi
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Study on the Application of Human Chorionic Gonadotropin (hCG) in Dairy Cow Superovulated with Pregnant Mare Serum Gonadotropin - Monoclonal Antibody (PMSG-MoAb) Anti PMSG Iman Supriatna; Tuty Laswardi Yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 5 No. 2 (1998): Media Veteriner
Publisher : Media Veteriner

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The research was planned to yield the biologic potential synergism of MoAb-hCG in increasing the embryo number. Twenty five dairy cows were divided into five groups. The Control Group was superovulated with 2,500 IU PMSG intramuscularly (i.m.), the remaining of the Group II, III, IV and V consecutively after being superovulated by 2,500 IU PMSG were treated with MoAb intravenously (i.v.), hCG i.v., MoAb-hCG i.v. and MoAb i.v-hCG i.m. Data evaluation were analyzed using ANOVA and LSD test. The result showed that there were increasing in number of transferable embryo per donor produced (P<0.01) after application with either MoAb i.v. (9.6), hCG i.v. (9.4), MoAb-hCG i.v. (11.2), or MoAb i.v.-hCG i.m. (9.6). The Control Group produced only 2.4 embryo per donor. The combined MoAb and hCG in one treatment were not able to increase the embryo yield (P>0.05).Key words: MoAb, PMSG, hCG, superovulation, donor, embryo
The Use of Direct Transfer Method on Embryo Cryopreservation in Dairy Cattle Iman Supriatna; Tuty Laswardi yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

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The experiment was carried out to study the use of direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-fourmorula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0,8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.5 M sucrose were 80.0, 80.8, 90.9 and 81.80% respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0,8 M (90.0%) sucrose was significantly better compared to those without (22.2%) or with 0.2 M (36.3%) sucrose (p
Melacak Antigen Virus Gumboro pada Bursa Fabrisius dan Limpa Menggunakan Metode Elisa (DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS ANTIGEN IN BURSA OF FABRICIUS AND SPLEEN USING ELISA) Anak Agung Ayu Mirah Adi; Hernomoadi Huminto; Lies Parede Hernomoadi
Jurnal Veteriner Vol 2 No 4 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Co-Authors Anak Agung Ayu Mirah Adi Bambang Purwantara Gozali Moekti Hernomoadi Huminto Iman Supriatna Tuty Laswardi Yusuf