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All Journal HAYATI Journal of Biosciences Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Jurnal Kedokteran Hewan
Tuty Laswardi Yusuf
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary

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Diurnal Patterns of Testosterone and Cortisol Metabolites in Fecal of Javan Gibbons (Hylobates moloch) in Captivity PUDJI ASTUTI; TUTY LASWARDI YUSUF; ERIC HAYES; HERA MAHESHWARI; LUTHFIRALDA SJAHFIRDI; DONDIN SAJUTHI
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (78.12 KB) | DOI: 10.4308/hjb.13.2.69

Abstract

The aims of this research were to determine diurnal patterns of testosterone and cortisol metabolites to predict the testis functional status. In this study, fecal testosterone and cortisol were quantified in 77 samples from three male Hylobates moloch during a course of three months period. These data showed that the highest concentration of fecal testosterone occured at 18.00-06.00 (23.61 ng/g dried feces), then declined gradually. The lowest concentration was in the evening (5.54 ng/g dried feces). Our tests showed that there was a decrease in the mean testosterone concentration from 06.00-10.00 to 10.00-14.00 to 14.00-18.00. For cortisol, the highest concentration occured at 06.00-10.00 (597.84 ng/g dried feces), then decline gradually in the evening (225.73 ng/g dried feces). Key words: Hylobates moloch, feces, testosterone, cortisol, diurnal pattern
Study on the Application of Human Chorionic Gonadotropin (hCG) in Dairy Cow Superovulated with Pregnant Mare Serum Gonadotropin - Monoclonal Antibody (PMSG-MoAb) Anti PMSG Iman Supriatna; Tuty Laswardi Yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 5 No. 2 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The research was planned to yield the biologic potential synergism of MoAb-hCG in increasing the embryo number. Twenty five dairy cows were divided into five groups. The Control Group was superovulated with 2,500 IU PMSG intramuscularly (i.m.), the remaining of the Group II, III, IV and V consecutively after being superovulated by 2,500 IU PMSG were treated with MoAb intravenously (i.v.), hCG i.v., MoAb-hCG i.v. and MoAb i.v-hCG i.m. Data evaluation were analyzed using ANOVA and LSD test. The result showed that there were increasing in number of transferable embryo per donor produced (P<0.01) after application with either MoAb i.v. (9.6), hCG i.v. (9.4), MoAb-hCG i.v. (11.2), or MoAb i.v.-hCG i.m. (9.6). The Control Group produced only 2.4 embryo per donor. The combined MoAb and hCG in one treatment were not able to increase the embryo yield (P>0.05).Key words: MoAb, PMSG, hCG, superovulation, donor, embryo
The Use of Direct Transfer Method on Embryo Cryopreservation in Dairy Cattle Iman Supriatna; Tuty Laswardi yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiment was carried out to study the use of direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-fourmorula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0,8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.5 M sucrose were 80.0, 80.8, 90.9 and 81.80% respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0,8 M (90.0%) sucrose was significantly better compared to those without (22.2%) or with 0.2 M (36.3%) sucrose (p
Morfometri, Morfologi Serta Abnormalitas Spermatozoa Owa Jawa (Hylobates moloch) Asal Ejakulat Pudji Astuti; Tuty Laswardi Yusuf; Dondin Sajuthi; Eric Hayes; Teguh Budipitojo; Luthfiralda Sjahfirji; Hera Maheshwari
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1474.497 KB) | DOI: 10.22146/jsv.486

Abstract

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Penentuan Siklus Estrus pada Kancil (Tragulus javanicus) Berdasarkan Perubahan Sitologi Vagina Najamudin -; Rusdin -; Sriyanto -; Amrozi -; Srihadi Agungpriyono; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the estrus cycle and the length of estrus in Tragulus javanicuson the basis of its vaginal cytology. Vaginal swabs were collected daily at 7 am for two months. Smears ofthe swab were then prepared on glass slide and they were stained with Giemsa. Vaginal epithelial cells;parabasal, intermediet and superficial cells were counted and their percentages during proestrus, estrusand diestrus were determined. Diestrus was characterized by the absent of superficial cells in the vaginalepithel. Proestrus was characterized by the progressive increase in percentage of intermediet/superficialcells in vaginal epithel, whereas estrus was characterized by the presence of superficial/cornification cellsin most vaginal epithel. On the basis of its vaginal cytology, it was determined that the estrous period offemale Tragulus javanicus was 3 days (48-60 h) and the length was 11 days (10-14 day). The change in thecytology of vaginal epithelial cells appeared to a good marker to determine the estrus cycle phase of theTragulus javanicus
Dinamika Ovarium Selama Siklus Estrus pada Domba Garut (OVARIAN DYNAMIC DURING THE ESTROUS CYCLE IN GARUT EWES) Satya Gunawan; Tuty Laswardi Yusuf; Mohamad Agus Setiadi; Arief Boediono; Rachmat Herman; Amrozi .
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Ovarian dynamics in the garut ewes had never been studied continuously by using ultrasonography.The aim of this study was to observe development of the follicles and corpus luteums in the estrous cyclein the garut ewes. Garut ewes (n=6) with body weight 30.00±4.05 kg which had normal estrous cycle wereused in this study. All ewes were synchronized by using CIDR-G implantation for 14 days. Ovulation of thedominant follicle, development of the follicle waves and corpus luteum were observed continuously everyday during the estrous cycle after CIDR-G removal. The number of small (2-3 mm in diameter), medium (4-5 mm in diameter) and large (>5 mm in diameter) follicles were aligned during the estrous cycle. Follicleand corpus luteum diameters were measured by using built in caliper in the ultrasound. The resultsshowed 1) the average length of estrous cycle was 19,2±0,8 days; 2) ovarian follicle growth occurred inthree waves during the estrous cycle; 3) the number of preovulatory follicles were 1-2 follicles; 4) theaverage maximum diameters of preovulatory follicle was 7.5±0.5 mm; 5) the average maximum diametersof corpus luteum was 7.3±0.4 mm. In conclusion, the estrous cycle in garut ewes was 18-20 days with 3follicular waves.
Penentuan Siklus Estrus Berdasarkan Gambaran Sitologi Vagina dan Profil Hormon pada Rusa Timor Wilmientje Marlene Mesang Nalley; Ristika Handarini; Muhammad Rizal; Raden Iis Arifiantini; Tuty Laswardi Yusuf; Bambang Purwantara
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Determination of estrous cycle especially the length of estrous is an important role in improvingthe fertility and reproductive performance of farm animals. This study was aimed to observe thelength of estrous cycle and estrous period based on cytologic and estrogen (E2) and progesterone (P4)hormone profiles. Samples of vaginal smears were collected daily and stained with Giemsa. Bloodsamples were collected every two days using jugular vein implant catheter. The hormone profileswere analized by radio immuno assay. All data were analyzed descriptively. Results of this studyshowed that there were four lowest points percentage of parabasal epithelial cells with the averageof 14.25%, while the superficial epithelial cells showed four highest points (85.75%). The intervalsbetween lowest point of parabasal epithelial cell and the highest point of superficial cells were at 20,18, and 16 with the average of 18 days. This phenomenon showed a cyclical processed with almostthe same length of time of the actual an estrous cycle pattern in timor deer. The range of E2 concentration was 7.06 to 18.14 pg/ml and P4 concentration was 2.58 to 7.48 ng/ml. The intervaltime between the peak of E2 was 17 days which represented the estrous cycle of timor deer. It isconcluded that analysis of estrous cycle in timor deer can be detected by vaginal cytological andhormone analysis profiles.
Sinkronisasi Estrus dan Inseminasi Buatan pada Rusa Timor Wilmientje Marlene Mesang Nalley; Ristika Handarini; Raden Iis Arifiantini; Tuty Laswardi Yusuf; Bambang Purwantara; Gono Semiadi
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this experiment was to examine the fertility of timor deer (Rusa timorensis) frozensemen by artificial insemination (AI) with intracervical technique after estrus synchronization withControlled Internal Drug Release, for Goat (CIDR-G®). Six adult, healthy, and cycling hinds aged 3 to 4years were used in this experiment. The percentage of estrus hind, onset and duration of estrus wereobserved. Pregnancy diagnosis was conducted using ultrasound scanner (USG) at day 120 afterinsemination. The response of estrus was 82.22 %, the onset of estrus was 25.33 hours after CIDR®withdrawal with the length of estrus was 28 hours. The pregnancy rate at first AI trial under sedation was16.7% (1/6), at second and third without sedation was 60% (3/5) and 100% (2/2) respectively. The durationof pregnancy was 248 to 285 days; with the average calving rate was 50%. The result concludes thatinsemination in hind without sedation was better than with sedation.
Kualitas Semen Cair Kambing Peranakan Etawah dalam Modifikasi Pengencer Tris dengan Trehalosa dan Rafinosa (THE QUALITY OF ETAWAH CROSSBREED BUCK LIQUID SEMEN IN MODIFIED TRIS DILUENTS WITH TREHALOSE AND RAFFINOSE) Oriza Savitri Ariantie; Tuty Laswardi Yusuf; Dondin Sajuthi; Raden Iis Arifiantini
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to compare the efficacy of trehalose and raffinose supplementation in Trisegg yolk (TEY) and Tris soya (TS) diluents in optimizing the quality of Etawah Crossbreed liquid semen.Semen were collected from three sexually mature bucks using artificial vagina. Semen were then evaluatedand divided into six aliquot tubes. Each of them was diluted in TEY and TS supplemented with 50 mMtrehalose or raffinose, respectively. The liquid semen were then stored in refrigerator (5°C). The motility,viability and plasma membrane integrity (PMI) of the spermatozoa were evaluated every 12 hours untilsperm motility remained up to 50%. The results showed that sperm motility in TEY supplemented withtrehalose or raffinose remained up to 50% for 72-84 hours, compared to in TS which was for 48-60 hours(P<0,05). The best diluent was demonstrated by TEY supplemented with trehalose where the spermmotility was 52,82±3,21% up to 84 h compared to raffinose supplementation (52,78±4,41%) and control(51,78±4,86%) which was up to 72 hours, respectively. Meanwhile, the spermatozoa motility in TS diluentsupplemented with raffinose was 52,78±4,41% for up to 60 hours compared to supplemented with trehalose(53,33±3,54%) and control (51,11±4,86%) which was up to 48 hours. In all diluents, the viability ofspermatozoa was 6-9% higher than the percentage of sperm motility, whilst the percentage of PMI wassimilar to the percentage of sperm motility. In conclusion, TEY supplemented with trehalose was the bestdiluents for preservation of Etawah Crossbreed buck liquid semen, and when using TS diluent it isrecommended to add raffinose  rather than trehalose.
Histologi dan Histomorfometri Testis dan Epididimis Muncak (Muntiacus muntjak muntjak) pada Periode Ranggah Keras (HISTOLOGY AND HISTOMORPHOMETRY OF THE TESTIS AND EPIDIDYMIS OF MUNTJAC (MUNTIACUS MUNTJAK MUNTJAK) DURING HARD ANTLER PERIOD) Sri Wahyuni; Srihadi Agungpriyono; Muhammad Agil; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to describe the histology and histomorphometry of testis and epididymisof muntjac (Muntiacus muntjak muntjak) during hard antler period. The tissues of the testis and epididymisof an adult male muntjac were processed for histological examination and stained with haematoxylineosine(HE). The parenchyma of muntjac’s testis during hard antler period showed tubuli seminiferi waslined with germinal epithelium: spermatogonia, spermatocyte, spermatid that differentiated intospermatozoa. Sertoli cells were found among the germinal cells. In addition, Leydig cells were foundaround the blood vessel of interstitial tissue along with macrophages. Diameter of the seminiferous tubuleand epithelial thickness were 176,60±7,06 ?m and 50,27±3,62 ?m respectively. The epididymal duct wassubdivided into three segments: caput, corpus and cauda. They were lined predominantly withpseudostratified columnar epithelium which was varied in its thickness. The largest diameter of epididymalduct was found in cauda region (324,26±25,79 ?m), while caput epididymidis had the thickest of epithelialcell (62,21±4,21 ?m) and tended to ce thinner in corpus (49,53±3,01 ?m) and cauda epididymidis(16,30±2,27?m). The density of spermatozoa was observed the most in the lumen of cauda region comparedto caput and corpus epididymidis. In conclusion, the structure of histology and histomorphometry of theseminiferous tubule of testis and epididymal duct of muntjac were similar with small ruminants andother Cervidae during hard antler period.
Co-Authors Amrozi Anak Agung Gede Sugianthara Arief Boediono Bambang Purwantara Dondin Sajuthi ERIC HAYES Faisal Amri Satrio GONO SEMIADI Gozali Moekti HERA MAHESHWARI Iman Supriatna Kim Chwin Khye Lies Parede Hernomoadi LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji M Agus Setiadi MUHAMMAD AGIL Ni Wayan Kurniani Karja Oriza Savitri Ariantie PUDJI ASTUTI R. Iis Arifiantini Rachmat Herman Reni Ratni Dapawole Ristika Handarini Rusdin - Satya Gunawan Solly Aryza Srihadi Agungpriyono Sriyanto - Teguh Budipitojo Wahyuni, Sri Wilmientje Marlene Mesang Nalley Yudi Eka Satria