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All Journal KUKILA Jurnal Agroekoteknologi JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Giyanto .
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Agriculture, Biological Sciences & Forestry Other

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Breeding Records of Little Egret Egretta garzetta in Sumatra, with notes on the occurence of race E. g. garzetta Muhammad Iqbal; Agus Nurza; Giyanto .
KUKILA Vol. 16 (2012)
Publisher : Indonesian Ornithologists’ Union

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Abstract

IDENTIFIKASI TOMATO INFECTIOUS CHLOROSIS VIRUS PENYEBAB PENYAKIT KLOROSIS PADA TANAMAN TOMAT DI CIPANAS JAWA BARAT MELALUI PERUNUTAN NUKLEOTIDA GEN PROTEIN SELUBUNG UTAMA Fitrianingrum Kurniawati; Gede Suastika; Giyanto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.34 KB) | DOI: 10.23960/j.hptt.11533-43

Abstract

Identification of tomato infectious chlorosis virus, the causal agent of chlorosis disease on tomato in Cipanas West Java by sequencing of main coat protein gene nucleotide. Tomato infectious chlorosis virus (TICV) causes chlorosis on tomato. Tomatoes infected by this virus shows interveinal yellowing, necrotic, bronzing, brittleness, and declining in productivity. This study aims to identify the causal agent of chlorotic disease on tomato by sequencing the coat protein gene. The methods involve collecting infected plants, total RNA extraction, cDNA synthesis, DNA amplification, visualization of the results of reverse transcription polymerase chain reaction (PCR), and phylogenetic analysis using BLAST, clustal w, Bioedit v 7.0.5.3, MEGA v 6:06. RT-PCR using spesific primers (CP-F TICV Bam and TICV R-Hind) amplified a DNA band of 792 bp, which has been successfully sequenced and identified as TICV. Nucleotide sequences homology analysis showed that TICV Indonesia_TWJ isolate Cipanas is the same strain as TICV from other countries (99.4 – 100%), such as Spain, Greece, USA, France, and Italy.
EKSPRESI GEN PROTEIN SELUBUNG TOMATO INFECTIOUS CHLOROSIS VIRUS PADA ESCHERICHIA COLI Fitrianingrum Kurniawati; Gede Suastika; Giyanto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.527 KB) | DOI: 10.23960/j.hptt.215114-121

Abstract

Expression of tomato infectious chlorosis virus coat protein gene on Escherichia coli. Tomato infectious chlorosis virus (TICV) is the causal agent of chlorotic disease of tomato. Detection of TICV can be carried out by RT-PCR and serological test. Titer of TICV in plant tissue is very low because TICV is limited to phloem. Serological detection of TICV requires antiserum which is not available in Indonesia. Producing antibody through cloning and coat protein gene (TICV CP gene) expression is a promising approach in producing antiserum. The objective of this study was to express TICV CP gene as antigen for antiserum production. TICV CP gene was amplified using RT-PCR from total RNA extracted from TICV infected leaves collected from Cipanas, Cianjur, West Java. The amplified CP gene was then sequenced and sub-cloned into pET 21b expression vector, transformed into Escherichia coli strain BL21 DE3(pLysS) and induced expression using IPTG 1 mM overnight at 37 °C. CP that contains 6xhistag was purified using NiNTAspin column and then confirmed by SDS-PAGE. The size of TICV CP gene was 750 bp and the gene was expressed on pET 21 b vector and SDS-PAGE showed a 29 kDa band.
EKSPLORASI DAN UJI SENYAWA BIOAKTIF BAKTERI AGENSIA HAYATI UNTUK PENGENDALIAN PENYAKIT KRESEK PADA PADI Sri Kurniawati; Kikin Hamzah Mutaqin; Giyanto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.946 KB) | DOI: 10.23960/j.hptt.215170-179

Abstract

Exploration of bacterial biocontrol agent and its potential bioactive compound to control rice bacterial leaf blight. The research aims were to obtain bacterial isolates which were potential as biological control agent of X. oryzae pv. oryzae, the causal agent of rice bacterial blight and to assess the effectiveness of their bioactive compounds, and to identify of the potential isolates. The research steps included bacterial isolation, screening based on antibiosis activity and pathogenicity test, characterization based on chitinolytic enzyme production, siderophores, and phosphate dissolution test, effectiveness test of bioactive compounds and molecular identification of potential isolates. Out of 156 bacterial isolates from rice crop tested, 11 isolates showed to be non plant pathogenic and to have activity as biological agents against X. oryzae pv. oryzae pathotype III, IV and VIII. Further characterization of 11 isolates resulted in 2 isolates that showrd ability to produce chitinase (isolates T5-1118 and R7-1018), phosphatase (isolates T5-1105 and T6-1109), and siderophores (isolates T5-1118 and T6- 1109). The test of bioactive compound effectiveness of 4 isolates to the growth of X. oryzae pv. oryzae showed thatT5-1118, T5-1105, T6-1109 and R7-1018 have ability to inhibit X. oryzae pv. oryzae at 48 hours after inoculation of 66,61%, 62,4%, 23,97% and 12,40%, respectively. Identification of 4 bacterial biocontrol isolates with partial sequencing of 16S rRNA gene showed that those bacteria are close to Bacillus nealsonii strain F22 (R7-1018), Chromobacterium sp. MWU328 (T5-1118), Streptomyces sp. Antag 1 (T5-1105) and Kitasatospora nipponensis strains H2-4 (T6-1109).
EFEKTIVITAS EKSTRAK TUMBUHAN MENGELIMINASI Clavibacter michiganensis Saubsp. Michiganensis DAN MEMPERTAHANKAN MUTU FISIOLOGIS BENIH TOMAT Aprizal Zainal; Aswaldi Anwar; Sudarsono .; Satriyas Ilyas; Giyanto .
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.841 KB) | DOI: 10.33512/j.agrtek.v3i1.567

Abstract

The objectives of the experiment were to evaluate effectiveness of seed treatments to eliminate Cmm of infected tomato seed lot, evaluate physiological quality of Cmm infected tomato seed lot after treatments with plant extracts. The research used curcuma rhizomes, betel vine leaf extract, cinnamon and clove oil to eliminate Cmm in tomato seed. Part of the seeds were dipped in either suspension of curcuma rhizomes, betel vine leaf extract, cinnamon and clove oil for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 0C and RH 60-70%) plus either of plant extracts or oils. Elimination of Cmm from infected seeds was observed 10 days after treatments, and physiological seed qualities were evaluated before and after seed treatments. Results of the experiment indicated, dipping infected seeds in either 5% of curcuma, betel vine extract, cinnamon oil or 0.5% of clove oil suspension or matriconditioning plus either these extracts, eliminated 99% of infected Cmm on tomato seeds. Seed treatments using plant extracts for elimination of Cmm did not reduce seed germination, germination rate, vigor index, and did not increase time to reach 50% total germination (T50) of infected tomato seed except the seed treatment by using 5% cinnamon oil with or without matriconditioning.Key words: plant extracts, physiological quality, seed viability, vigor
Co-Authors Agus Nurza Anwar, Aswaldi Aprizal Zainal Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika Kikin H Mutaqin Muhammad Iqbal Satriyas Ilyas Sri Kurniawati Sudarsono