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Produksi, Karakterisasi, dan Isolasi Lipase dari Aspergillus niger menggunakan Minyak Goreng Sawit sebagai Induser Sri Wahyu Murni; Siti Diyar Kholisoh; Tanti D.L.; Petrissia E.M.
Eksergi Vol 12, No 1 (2015)
Publisher : Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/e.v12i1.1034

Abstract

The objective of the research was to produce, isolate and characterise lipase from Aspergillus niger, and therefore inducted it by using palm oil. The lipase enzyme was produced through a batch fermentation process in a 1.4 liters-fermentor. Fermentation was carried out at room temperature, initial pH of 7, the stirring speed of 250 rpm, aeration rate of 1 vvm, and inducer concentration of 3%-m/v palm oil/ml. Enzymes was characterised at several temperature and pH variations. The lipase showed the optimum performance at pH of 7 and temperature of 30 °C with the activity of 1.5 U / ml. Isolation of lipase yielded a 4-times-increase in its activity by using 90% ammonium sulfate.
Peningkatan Phycocyanin pada Spirulina Platensis dengan Media Limbah Virgin Coconut Oil pada Photobioreactor Tertutup Sri Sukadarti; Sri Wahyu Murni; M.M. Azimatun Nur
Eksergi Vol 13, No 2 (2016)
Publisher : Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/e.v13i2.1700

Abstract

Waste of Virgin Coconut Oil (VCO) industries has a high value of Chemical Oxygen Demand (COD), it is therefore a problem for the environment. This waste contains organic materials such as oil, proteins, carbohydrates and some minerals, that potentially to be used for the cultivation of blue-green algae Spirulina plantesis.  Spirulina plantesis is an excellent source of phycocyanin. The Phycocyanin has anti-aging, anti-oxidant and anti-inflammatory properties, it is therefore considered useful. This research is aimed to study the effects of the addition of urea and light intensity on the growth of Spirulina, the concentration of phycocyanin and decreasing of COD value. Spirulina platensis was cultivated in a closed photobioreactor with an air flow for 7 days.  The light intensity was varied as follows 5000 lux, 6000 lux, 7000 lux and 8000 lux, and the addition of urea as nutrients was also varied as follows 40 ppm, 50 ppm, 60 ppm and 70 ppm.  This research indicated that the optimum condition was obtained at the addition of urea of 70 ppm, light intensity  of 6000 lux. This research resulted μmax of 1,1375day-1 , the biomass  productivity of 0,0423 g/l/day,  biomass concentration of   0,1734 g/l  and  phycocyanin concentration of 5,047%. The largest of COD removal is was 98,06% and the COD value of  280 mg/l was finally achieved.
Effect of particle sizes and sodium hydroxide concentrations on silica extraction from minerals obtained in Kalirejo village, Kokap, Kulonprogo, Yogyakarta Tutik Muji Setyoningrum; Wibiana Wulan Nandari; Sri Wahyu Murni; Muhamad Maulana Azimatun Nur
Eksergi Vol 18, No 1 (2021)
Publisher : Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/e.v0i0.4576

Abstract

Silica is mainly used in the production of rubber, glass, cement, glass, ceramics, paper, cosmetics, electronics, paintings, healthcare and other industries. Kalirejo village has a potential abundant resource of silica minerals. However, to refine it, high cost of extraction should be done to obtain high purity of silica. Different refining methods influence different purity of the silica. The purpose of this research was to study the refining process of mineral rock silica from Kalirejo village, Kokap, Kulonprogo by emplying simple and cheap solid-liquid extraction. Extraction was done by varying the particle size at 100 - 200 mesh, while NaOH concentration was varied in 0.5 N to 5 N.  Results showed that minerals taken from Kalirejo village was dominated by silica (23%).  The largest extract (4.89 gram) was obtained at 200 mesh and using NaOH 5 N with yield of 15.07%. Higher NaOH and higher particle size enhanced the extraction yield. This finding could help small communities in Kalirejo village to enhance the quality of silica by employing simple and cheap extraction process.
Pengaruh Aktivator Stardec terhadap Proses Pembuatan Pupuk Organik dari Kotoran Sapi Tunjung Wahyu Widayati; Sri Wahyu Murni; Afika Syahliana Sriadi; Deka Prima Rosalinda
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2017: PROSIDING SNTKK
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Cow’s feces originated from farms dairy an organic waste that can causing an environmental pollutiontherefore need to be process to make something such as fertilizer using activators that help the decompositionprocess run faster. Activator used in this experiment is Stardec. Decomposition of cow’s feces becomingorganic fertilizer by mixing cow’s feces with Stardec activator. Ratio of cow’s feces weight (Metric Ton) byweight (Kilogram) of Stardec activator is 1:0; 1:1; 1:2; 1:3; 1:4. Thereafter, add the anorganic fertilizer(Urea, KCl, SP-36) and limestone to the mixture each about 2,5 kilograms after that cover the mixture withplastic wrap and make some holes with bamboo. The allowing the mixture for 3 weeks to make an analysis ontemperature and C/N ratio for 3 days. The experiment result shows that process of composition for 21 dayson Stardec with 10 kg weight to produce organic fertilizer consist about 15,23% organic carbon, 0,84% totalnitrogen, 18,13 C/N ratio. These result meet the quality standards set by the Minister of AgricultureNo.70/Permentan/SR.140/10/2011.
Pengaruh Aktivator Effective Microorganism 4 Terhadap Proses Pembuatan Pupuk Organik dari Kotoran Sapi Sri Wahyu Murni; Tunjung Wahyu Widayati; Bassmall Andriouty Pratama; Satriyo Oktavianto
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2017: PROSIDING SNTKK
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Cow's excrements originated from slaughterhouse is an organic waste that can damage the environmenttherefore need to be process to make something such as fertilizer using activators that help thedecomposition process run faster. Activator used in this experiment is Effective Microorganism 4.Decomposition of cow’s excrements becoming organic fertilizer by mixing cow’s excrements with EffectiveMicroorganism 4 activator. Ratio of cow’s excrements weight (Metric Ton) by volume (Liter) of EffectiveMicroorganism 4 activator is 1:0; 1:1; 1:2; 1:3; 1:4. Thereafter, add the anorganic fertilizer (Urea, KCl,SP-36) and limestone to the mixture each about 2,5 kilograms after that cover the mixture with plastic wrapand make some holes with bamboo. The experiment result for this study using Effective Microorganism 4activator by adding 4 liter in the decomposition process for 18-21 days to produce organic fertilizer consistabout 15,01% organic carbon, 0,87% total nitrogen, 17,25 C/N ratio. These result meet the qualitystandards set by the Minister of Agriculture No.70/Permentan/SR.140/10/2011.
Production of briquettes from Indonesia agricultural biomass waste by using pyrolysis process and comparing the characteristics Sri Wahyu Murni; Tutik Muji Setyoningrum; Muhamad Maulana Azimatun Nur
Eksergi Vol 18, No 1 (2021)
Publisher : Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/e.v0i0.4572

Abstract

Indonesia biomass waste is a potential feedstock as a source of renewable energy since it can be converted into carcoal briquettes. However, the production of the briquettes using pyrolysis process using the agricultural waste was lacking. In this research, briquette was made from palm shells,  corncob and soybean stem wood due to its high availability and have high cellulose content. The purpose of this research was to produce briquettes from three kind of raw materials by employing pyrolysis process and compared the characteristics. The briquette was made from different type of raw materials (palm shells, corncob and soybean stem) and  the concentration of binder : 3-7 %. Pyrolysis was done at  500 °C, and 100 kg / cm2 of pressing pressure. Results showed that, the best charcoal briquette was achieved from palm shells by using 5% binder, which resulted 4,1% moisture content, 3.4% ash content,  15% volatile matter content, 77.5% carbon content,  7075 cal/g calorific value and  1.4 kg/cm2 compresive strength. It is found that the concentration of binder and raw material influenced the quality of the briquettes. In overall, the production of the briquettes by employing pyrolysis method could meet the standard.
KOPOLIMERISASI CANGKOK METIL METAKRILAT PADA PATI TAPIOKA MENGGUNAKAN INISIATOR CERIC AMMONIUM NITRAT Sri Wahyu Murni
Eksergi Vol 10, No 1 (2010): Versi Cetak
Publisher : Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/e.v10i1.7614

Abstract

Telah dilakukan kopolimerisasi cangkok antara tepung pati tapioka dengan metil metakrilat menggunakan inisiator cerric ammonium nitrat, hasilnya berupa kopolimer cangkok pati-metil metakrilat/ Starch-graft-Polimetil Metakrilat (S-g-PMM). S-g-PMM yang dihasilkan dari sintesis ini merupakan produk yang dapat dimanfaatkan sebagai penyerap air, selain itu kopolimer ini dapat digunakan sebagai material pengganti yangbersifat biodegradable. Penelitian dilakukan dengan mencampurkan pati tapioka yang dilarutkan dalam air dan diaduk sampai homogen dalam labu leher tiga. Kemudian ditambahkan inisiator inisiator cerric ammonium nitrat (CAN) yang telah dilarutkan dalam HNO3 0,5 N, serta monomer metil metakrilat. Setelah reaksi selesai ditambahkan metanol. Padatan S-g-PMM yang diperoleh diuji strukturnya menggunakan FTIR, daya serap air dan daya urainya Hasil menunjukkan bahwa pada perbandingan 8,5 g tepung tapioka dengan 12 ml metil metakrilat (perbandingan MM:pati tapioka =1,3201:1), suhu 40oC, dihasilkan S-g-PMM dengan yield 41,74%, kapasitas absorbsi air 375% dan kemampuan terurai secara alami 6,42%.
Polimerisasi Propilena menggunakan Katalisator TiCl4 dan Kokatalis Tri Etil Aluminium Sri Wahyu Murni
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2010: Prosiding SNTKK 2010
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Bio-diesel Production from Kapok Seed Oil Sri Wahyu Murni
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2009: Prosiding SNTKK 2009
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Produksi Enzim Selulase dari Aspergillus niger Secara Fermentasi dalam Medium Kultur Padat Sri Wahyu Murni
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2012: Prosiding SNTKK 2012 (Arsip)
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Bioethanol made from lignocellulosic biomass is currently being developed. The main obstacle production of bioethanol from lignocellulosic materials is at the stage of the process of hydrolysis of lignocellulosic material to form sugars. Enzymatic hydrolysis using cellulase enzyme is plagued by an expensive price. In the previous studies have been carried out production of Trichoderma reesei cellulase enzymes,in this research conducted using Aspergillus niger by solid substrate fermentation method. Enzyme production studies conducted in two stages in, there were in the aerated Erlenmeyer flask and rotary drum fermenter (RDF). The results showed that the production of cellulase enzymes in the rice straw substrate has resulted in enzyme activity similar to sawdust from the wood sengon. Water content effect on enzyme activity. The best results were obtained on a substrate of rice straw at 60% moisture content and fermentation time of 6 days, the CMC-ase activity of FP-ase was 0.00229 and 0.00095U /ml respectivelly. The best results in sengon sawdust substrate is reached at 70% moisture content and fermentation time of 7 days, obtained CMC-ase and FP-ase activity 0.00257 and 0.00133 U/ml respectivelly. Productivity of the enzyme in the RDF is similar to the Erlenmeyer flask. At RDF, obtained CMC-ase and FP-ase of 0.00243 and 0.00105 U / ml for rice straw substrate, and 0.00224 and 0.00125 U / ml for sengon sawdust substrate.