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. Purwoko
Faculty of Medicine, Sebelas Maret University
Biochemistry, Genetics & Molecular Biology

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Efektivitas Desinfektan Karbol 4% di Ruang Isolasi Barat ICU RSUD Dr. Moewardi Brenda Ervistya Pertiwi; . Hudiyono; . Purwoko
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Nosocomial infection still becomes a global health problem. An attempt to reduce the risk of nosocomial infections in the ICU (intensive care unit) room of RSUD Dr. Moewardi, 4% carbol has been used routinely in disinfection. This study was to know the effectiveness of 4% carbol disinfectant in West Isolation Room of ICU in RSUD Dr. Moewardi. This study was an analytical observational research with a cross sectional approach. The samples collected from ICU resulted from three groups. Each group was 15 samples, collected from swabbing the floor and the wall (grown on agar), and from the air (grown on blood agar). For each group, 5 samples were taken at 6 am (1 hour before disinfection), at 9 am (2 hours after disinfection) and at 12 pm (5 hours after disinfection). The data were analyzed using Wilcoxon experiment Based on the standard of germs limit on wall, floor (5-10 colony/cm2) and air (?200 cfu/m3) at intensive care unit, the result from swabbing the wall and air samples showed that the disinfection was 100% effective. Whereas, the result from swabbing the floor was only 13.33% effective. Between germs number from swabbing the wall collected at 6 am and 9 am represented a significant reduction (p = 0.042). On the other hand, there was no significant reduction of germ number between which were collected at 6 am and 12 pm (p = 0.068) as well as between 9 am and 12 pm (p = 1.000). In addition, there was also no significant reduction of germs number resulting from swabbing the floor between collected at 6 am and 9 am (p = 0.080); 6 am and 12 pm (p = 0.080); and 9 am and 12 pm (p = 0.068). Furthermore, the number of air germs between collected at 6 am and 9 am represented a significant reduction (p = 0.043). But, there was no significant reduction between collected at 6 am and 12 pm (p = 0.225), as well as at 9 am and 12 pm (p = 0.715). Disinfection with 4% carbol in west isolation room of ICU RSUD Dr. Moewardi showed effectively controlling germs number on the wall and the air, but it was not effectively controlling on the floor. Keyword: disinfectant, carbon 4%, germs number.
Skrining Methicillin Resistant Staphylococcus aureus (MRSA) pada Tenaga Kesehatan Bangsal Melati 1, Melati 2, dan Mawar 2 RSUD Dr. Moewardi Achmad Faiz Sulaiman; . Marwoto; . Purwoko
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: MRSA is known as one of main cause of nosocomial infection since found in 1961. In Indonesia, its prevalence reached 23,5%. Direct contact to healthcare workers can cause transmission of Staphylococcus aureus to patients. Carrier of chronic MRSA plays important roles on MRSA infection in hospital setting. This research aimed to know the incidence of MRSA nasal carriage of healthcare workers in RSUD Dr Moewardi Surakarta. Methods: This was an descriptive study using cross sectional design. Samples were taken from nasal swab of healtcare workers in wards of Melati 1, Melati 2 and Mawar 2. Samples were examined in Microbiology Laboratory of FK UNS. Total samples obtained were 74 according to total sampling theory. Data were analyzed descriptively and tested with Chi-Square tests. Results: The result of nasal swab from 26 healthcare workers in Melati 1 ward showed that there were 7 healthcare workers that were colonized by Staphylococcus aureus and one of them was MRSA case. In Melati 2 ward, from 25 healthcare workers, ten of them were colonized by Staphylococcus aureus and 8 out of 10 healthcare workers were MRSA cases. Whereas in Mawar 2 ward from nasal swab of 23 healthcare workers, six of them were colonized by Staphylococcus aureus and no MRSA case was found. Conclusions: The number of MRSA incidence in Melati 1, Melati 2 and Mawar 2 wards consecutively were 1.33%, 10.67% and 0%. Keywords: MRSA, healthcare worker, colonization.
Uji Sterilitas Instrumen Bedah Selama Penyimpanan di Kamar Operasi IGD RSUD Dr. Moewardi Rizki Banjar Kurniawan; . Hudiyono; . Purwoko
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Surgical Site Infection (SSI) in RSUD Dr. Moewardi still occurs frequently. SSI strongly relates to the sterilization quality of surgical instrument which is determined by the stage of sterilization process that includes cleaning, packaging, ordering in sterilizer, sterilizing process, distribution, and storage. The maximum storage length of sterile surgical instrument in IGD operating chamber of RSUD Dr. Moewardi is seven days. This research aimed to know the sterilization rate of surgical instrument stored in IGD operating chamber of RSUD Dr. Moewardi. Methods: Five samples were taken from post-steriliziation surgical instrument swab with varied storage length, those were 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, and 7 days. A total of 35 samples were collected based on the rule of thumb with simple random sampling technique. Data were analyzed using Chi Square test. Results: The result of 5 samples of surgical instrument swab after 1 day storage was 1 positive sample with contamination and 4 negative samples. From 5 samples of surgical instrument swab after 2 and 3 days storage, there was not any positively contaminated samples. Five samples of surgical instrument swab after 4 and 5 days storage showed 4 samples positively contaminated and 1 sample negative. From 5 samples of surgical instrument swab after 6 and 7 days storage, there were 3 samples positively contaminated and 2 samples negative. The microorganisms of contaminant found were gram positive bacteria. The statistical analysis showed a significant relationship between the length of surgical instrument storage and the contamination of microorganism (p = 0.017). Conclusions: There was a significant difference of bacteria growth rate on surgical instruments based on storage length. Surgical instruments stored in IGD operating chamber of RSUD Dr. Moewardi > 3 days were 33 times more likely contaminated than the surgical instruments stored ? 3 days (OR = 32,67; p < 0,001). Keywords: sterility, contamination of surgical instrument, storage length.
Co-Authors . Hudiyono . Marwoto Achmad Faiz Sulaiman Brenda Ervistya Pertiwi Rizki Banjar Kurniawan