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Azmi Azhari
Institut Pertanian Bogor
Biochemistry, Genetics & Molecular Biology Education

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Trametes versicolor as Agent for Delignification of Rice Husks Laita Nurjanah; Syamsul Falah; Azmi Azhari; Suryani Suryani; I Made Artika
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Abstract

Rice husks contains 33.71% w/w lignocelluloses, the most abundantly available raw material on the earth for the production of biofuels and other valuable products. It is comprised of the carbohydrate polymers, cellulose, hemicellulose, and an aromatic polymer, lignin. One of the methods for removing the lignin component of rice husks is by delignification using white-rot-fungi. The aim of the study was to carry out delignification of rice husks using white-rot-fungi. The white-rot-fungi used here were Trametes versicolor and Phanerochaete chrysosporium. The study consisted of a biomass and microbial preparation, chemical assay of the rice husk, ligninase enzyme tests, and delignification of rice husks. Results showed that T. versicolor and P. chrysosporium have ligninase enzyme. The precentage of lignin from the total biomass rice husks was 23.61% w/w, and following the delignification process by T. versicolor for 20 days, the remaining lignin was 16.20% w/w, making the percentage of rice husks lignin degraded as 7.41% w/w. The biodelignification process also decreased the percentage of holocellullose, cellulose, and other extracted substances, and accordingly this increased the percentage of hemicellulose. Based on the ability of T. versicolor to degrade lignin of the rice husk at room temperature (28 ºC) as mentioned above, it can be concluded that T. versicolor has potential to be used for delignification process.
Delignifikasi Batang Kayu Sengon oleh Trametes versicolor Azmi Azhari; Syamsul Falah; Laita Nurjannah; Suryani Suryani; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Delignification is a lignin degradation, a preliminary process in industries that used cellulose containing substrates. Sengon logs are often used for the material in pulp industry because it has high levels of cellulose and low level of lignin. The aim of this study was delignification of sengon logs by using T.versicolor. The methods used include observation growth of T.versicolor compared with Phanerochaete chrysosporium, the rate of of lignin degradation (black liquor), delignification of sengon logs using T.versicolor and the chemical assay of sengon logs before and after delignification. The results of this study showed that delignification by T.versicolor was faster compared to P.chrysosporium based on the rate of lignin degradation (black liquor). The result showed that delignification by T.versicolor at room temperature reduced lignin of sengon logs by 37.31% within 20 days. Chemical assay performed on delignified sengon wood showed decreased level of ethanol benzene, soluble extractive substances, holocellulose, and cellulose and an increase of hemicellulose level.
Co-Authors I MADE ARTIKA Laita Nurjanah Laita Nurjannah MARIA BINTANG Suryani Suryani Syamsul Falah