Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Ternak Veteriner Proceeding of International Conference Health, Science And Technology (ICOHETECH)
Ahmad Baidlowi
Universitas Gadjah Mada
Computer Science & IT Decision Sciences, Operations Research & Management Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Effect of Mix Culture Bacteria and Fungi in Fermented Peanut Hulls-Based Feed Supplement on Physical Quality and In Vitro Rumen Fermentation Parameters DELA RIA NESTI; Ahmad Baidlowi; Achmad Fauzi; Ida Tjahajati
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 1 (2020): MARCH 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i1.2079

Abstract

The purpose of this research was to determine the effect of the combination of mix culture bacteria (EM4®:E) and fungi (Trichoderma viridae:TV) on physical quality and in vitro rumen fermentation parameters of peanut hull-based feed supplements. Basal feed was divided into four treatments, which were: P0 (BF); P1 (E:25%+TV:75%); P2 (E:50%+TV:50%),; and P3 (E:75%+TV:25%); and each treatment had three replications. Feeds were fermented facultative anaerobically for nine days. The observed parameters were physical qualities (color, odor, fungi appearance and pH), and in vitro rumen fermentation parameters (rumen pH, microbial protein content, and total volatile fatty acid content). Data were analyzed using one way ANOVA design, and the significance of differences were tested using Kruskall-Wallis test for the physical qualities data and Duncan’s New Multiple Range Test (DMRT) test for in vitro rumen fermentation parameters. Results showed that the combination of E and TV at different level did not affect odor and the fungi appearance parameters (P≥0.05), however, it significantly affected P≤0.05) color change from blackish (1.64) in P0 to brownish in P1, P2 and P3 of 2.44; 2.69; and 2.80, respectively. The pH also decreased significantly (P≤0.05) by 10.67%.  Treatment also did not affect the rumen pH, microbial protein content, and total volatile fatty acid content (P≥0.05). It is concluded that the combination of the EM4® 25%: Trichoderma viridae 75% on peanut-hull based feed supplement fermentation gives the best result on color and pH fermented feed product without affecting the rumen fermentation process.
Metagenomic Sequencing of P30-gene-positive Using MinION Sequencer for Apicomplexan Species Identification Dela Ria Nesti; Ahmad Baidlowi; Lucky Ronald Runtuwene; Ari Indrawati; Wayan Tunas Artama; Yutaka Suzuki
Proceeding of International Conference on Science, Health, And Technology 2021: Proceeding of the 2nd International Conference Health, Science And Technology (ICOHETECH)
Publisher : LPPM Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (996.028 KB) | DOI: 10.47701/icohetech.v1i1.1068

Abstract

Oxford Nanopore Technologies MinION sequencer is a recent technology of portable, real-time, single molecule long-read DNA/RNA sequencing which has the potential to analyze clinical and environmental samples in the field. Here, we describe the result of metagenomic sequencing from samples with P30-gene-positive using MinION sequencer. One hundred cattle blood samples which were confirmed positive of blood parasites using blood smear method were collected from Diseases Investigation Centre (DIC), Yogyakarta province. After DNA extraction, each sample was amplified by conventional PCR using primers targeting the P30 gene. PCR products were visualized under UV illumination after electrophoresis on 1.5% TBE/agarose gels and stained with SYBR Safe™ DNA gel stain. Twenty-five samples showed the desired 400-bp DNA fragment, which was inferred as P30 gene positive. Four whole cattle blood samples with the highest DNA concentration were sequenced separately using MinION sequence for 24 hours. Sequencing data was then analyzed using bioinformatics tools. Three samples resulted in low sequencing yield and could not be analyzed, but we were able to identify Theileria orientalis as the major pathogen from one sample. We were also able to construct 95.8% of its genome. To our knowledge, this is the first publication of a parasite genome reconstruction from metagenomic sample using MinION sequencer in Indonesia.
Co-Authors Achmad Fauzi Ari Indrawati Ida Tjahajati Lucky Ronald Runtuwene Nesti, Dela Ria Wayan Tunas Artama Yutaka Suzuki