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All Journal Jurnal Sain Veteriner Jurnal Ilmu Ternak Veteriner Veterinary Biomedical and Clinical Journal Jurnal Nasional Teknologi Terapan Proceeding of International Conference Health, Science And Technology (ICOHETECH)
Nesti, Dela Ria
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

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Effect of Mix Culture Bacteria and Fungi in Fermented Peanut Hulls-Based Feed Supplement on Physical Quality and In Vitro Rumen Fermentation Parameters DELA RIA NESTI; Ahmad Baidlowi; Achmad Fauzi; Ida Tjahajati
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 1 (2020): MARCH 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i1.2079

Abstract

The purpose of this research was to determine the effect of the combination of mix culture bacteria (EM4®:E) and fungi (Trichoderma viridae:TV) on physical quality and in vitro rumen fermentation parameters of peanut hull-based feed supplements. Basal feed was divided into four treatments, which were: P0 (BF); P1 (E:25%+TV:75%); P2 (E:50%+TV:50%),; and P3 (E:75%+TV:25%); and each treatment had three replications. Feeds were fermented facultative anaerobically for nine days. The observed parameters were physical qualities (color, odor, fungi appearance and pH), and in vitro rumen fermentation parameters (rumen pH, microbial protein content, and total volatile fatty acid content). Data were analyzed using one way ANOVA design, and the significance of differences were tested using Kruskall-Wallis test for the physical qualities data and Duncan’s New Multiple Range Test (DMRT) test for in vitro rumen fermentation parameters. Results showed that the combination of E and TV at different level did not affect odor and the fungi appearance parameters (P≥0.05), however, it significantly affected P≤0.05) color change from blackish (1.64) in P0 to brownish in P1, P2 and P3 of 2.44; 2.69; and 2.80, respectively. The pH also decreased significantly (P≤0.05) by 10.67%.  Treatment also did not affect the rumen pH, microbial protein content, and total volatile fatty acid content (P≥0.05). It is concluded that the combination of the EM4® 25%: Trichoderma viridae 75% on peanut-hull based feed supplement fermentation gives the best result on color and pH fermented feed product without affecting the rumen fermentation process.
Effectiveness of Gout Therapy with Polyherbal Extract of Shallot (Allium ascalonicum L.) and Red Ginger (Zingiber officinale var rubrum) in Hyperuricemia Mice Fathur Rohman Haryadi; Dela Ria Nesti; Ida Tjahajati; Okti Herawati
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.51360

Abstract

Gout can cause inflammation of acute gout arthtritis, as well as other complications. Provision of chemical drugs in the long term have side effects. Shallots (Allium ascalonicum L.) contain flavonoids which can inhibit the xanthine oxidase enzyme, so as to reduce uric acid levels. Red ginger extract (Zingiber officinale var rubrum) is rich in gingerol to reduce uric acid with anti-inflammatory effect. Polyberbal combination is expected to increase the effectiveness of therapy. The research aims to determine the effectiveness of polyherbal combinations. 25 male rats (2-3 months) were divided into five groups, namely negative control, positive control, (P1) 25% red ginger extract: 75% shallot, (P2) 50% red ginger extract: 50% shallot, ( P3) 75% red ginger extract: 25% shallot. Induction of potassium oxonate 250 mg / kg BW intraperitoneally on the 7th day. The first measurement of uric acid on the 14th day with Urid Acid Toos, the 15th to 28th days was administered herbal / chemical drugs according to the group. Measurement of second and third uric acid on days 21 and 28. Data were analyzed by one-way Anova (RAL), further significance tests using Least Significant Different (LSD). The results showed that administration of potassium oxonate and combination of herbal extracts had a significant effect (p <0.05) on uric acid levels in all groups. The most effective herbal combination doses are (P3) 75% red ginger (450 mg / 200 gr) and 25% shallot (150 mg / 200 gr BW). The conclusion of this study is the combination of red ginger extract and shallot can be recommended as a uric acid-lowering agent.
Profil Glukosa Darah, Lipid dan Visualisasi Pulau Langerhans sebagai Imunoreaktor Insulin dan Glukagon pada Pankreas Tikus (rattus norvegicus) Obesitas Menggunakan Teknik Imunohistokimia Dela Ria Nesti; Ahmad Baidlowi
Jurnal Nasional Teknologi Terapan (JNTT) Vol 1, No 1 (2017): NOVEMBER
Publisher : Penelitian dan Pengabdian Kepada Masyarakat Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1027.35 KB) | DOI: 10.22146/jntt.34083

Abstract

Obesity is not concerned as specific congenital disorder or derived lipid metabolism disorder recently, but more to bod yinability to handle the increase of nutrition intake caused by behavioral changes. Langerhans Islet is a section in the pancreas which play crucial role in producing and regulating metabolism hormones, insulin and glucagon. This research was conducted to observe blood glucose and lipid profile and to visualize Langerhans Islet in rat pancreas which were induced obesity and compared it with normal ones. Ten of male non-genetic obese four weeks old Winstar rats were divided into two groups, normal (K) and induced with obesity (O). Blood plasma samples were collected to determine blood glucose and blood lipid profile. Pancreas (regio splenic) samples were collected and fixated with Bouin solution, blocked by paraffin and cut with 5 μm thickness. Histology preparats were stained with Hematoxylin-Eosin and Immunohistochemistry (IHC) is determined with Labeled Sterptavidin-Biotin (LSAB) method. Staining is act as visual detector with will be analyzed descriptively and quantitatively. The data of Obesity group showed that blood glucose level is 88.59 ± 1.77 mg/dl, blood cholesterol level is 160.62±3.52 mg/dl, triglycerides level is 98.61±2.66 mg/dl, HDL level is 44.68±2.25 mg/dl and LDL level is 67.51±1.81. Pancreas’s topography in obese group spread into three regions, which are: gastrium, duodenum and lien. Langerhans Islet morphology shaped oval and polygonal also surrounded by fine filaments and blood capillary. Insulin and glucagon immunoreactive cell’s morphology in obesity group shaped irregular polygonal with round/oval nucleus placed in central/peripheral. Langerhans Islet’s in obesity group has diameter (154.38±65.56) μm, circumference (373,51±146,69) μm and volume 3241105.96 μm3. Obesity induced rat showed the increase in cholesterol level, triglycerides level, LDL level, blood glucose level and Langerhans Islet’s diameter, volume and circumference compared to normal ones (P<0.05). Decrease of HDL, topography and morphology of pancreas in obesity induced rat did’t showed significant changes compared to normal ones.
Deteksi Penyakit Zoonosis Ehrlichiosis pada Pasien Anjing di Klinik Hewan Jogja Dela Ria Nesti; Ahmad Baidlowi; Febri Ariyanti; Ida Tjahajati
Jurnal Nasional Teknologi Terapan (JNTT) Vol 2, No 2 (2018): JULI
Publisher : Penelitian dan Pengabdian Kepada Masyarakat Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.646 KB) | DOI: 10.22146/jntt.42966

Abstract

Ehrlichiosis is a zoonotic diseases caused by blood parasitic infection that can be transmitted by a ticks. Dogs infected with Ehrlichiosis canis who not treated for long periods of time can cause anemia and result in death. Although many diseases are found in the field but the percentage rate of the incidence of the disease is unknown. This study aims to detect Ehrlichiosis disease in dog patients treated at Jogja Veteinary Clinic by using Ehrlichiosis diagnosis kit available in the field. The material used in this study used dog patients who came to the Jogja Veterinary Clinic during April to September 2017. Dog patients who showed signs of infection of blood parasites with clinical symptoms of appetite and drinking down, weak, lethargic, found the presence of a ticks, or there history of infected ticks was included in the study. Patients who entered the study criteria, then taken the blood through the vena cephalika by using a 3 ml sterile syringe. Furthermore, fresh blood drops on the Ehrlichiosis detection kit according to the manufacturer's instructions. Furthermore, the results are positive if found two lines and negative when only seen one line. Data of research result are presented in tabular form and percentage of disease according to result obtained. The results of the research showed that there were 7,63% (15/1,785) positive dogs suffering from Ehrlichiosis. These results can illustrate the incidence of Ehrlichiosis in dogs, especially in dog patients treated at the Jogja Veterinary Clinic, because of the danger of Ehrlichiosis (zoonotic), awarness is needed.
Metagenomic Sequencing of P30-gene-positive Using MinION Sequencer for Apicomplexan Species Identification Dela Ria Nesti; Ahmad Baidlowi; Lucky Ronald Runtuwene; Ari Indrawati; Wayan Tunas Artama; Yutaka Suzuki
Proceeding of International Conference on Science, Health, And Technology 2021: Proceeding of the 2nd International Conference Health, Science And Technology (ICOHETECH)
Publisher : LPPM Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (996.028 KB) | DOI: 10.47701/icohetech.v1i1.1068

Abstract

Oxford Nanopore Technologies MinION sequencer is a recent technology of portable, real-time, single molecule long-read DNA/RNA sequencing which has the potential to analyze clinical and environmental samples in the field. Here, we describe the result of metagenomic sequencing from samples with P30-gene-positive using MinION sequencer. One hundred cattle blood samples which were confirmed positive of blood parasites using blood smear method were collected from Diseases Investigation Centre (DIC), Yogyakarta province. After DNA extraction, each sample was amplified by conventional PCR using primers targeting the P30 gene. PCR products were visualized under UV illumination after electrophoresis on 1.5% TBE/agarose gels and stained with SYBR Safe™ DNA gel stain. Twenty-five samples showed the desired 400-bp DNA fragment, which was inferred as P30 gene positive. Four whole cattle blood samples with the highest DNA concentration were sequenced separately using MinION sequence for 24 hours. Sequencing data was then analyzed using bioinformatics tools. Three samples resulted in low sequencing yield and could not be analyzed, but we were able to identify Theileria orientalis as the major pathogen from one sample. We were also able to construct 95.8% of its genome. To our knowledge, this is the first publication of a parasite genome reconstruction from metagenomic sample using MinION sequencer in Indonesia.
The Effect of Clove's and Lemongrass's Essential Oil in Basal Feed on Performances and Hematology Profile of Streptozotocin-Induced Diabetic Rat Baidlowi, Ahmad; Nesti, Dela Ria; Tjahajati, Ida
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.456 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.02.1

Abstract

This research was conducted to determine the effect of Clove’s and Lemongrass’s essential oil (EO) in basal feed on performances and hematology profile of Sterptozotocin-induced diabetic rat. 20 rats aged 40 days were divided into four groups of feed treatments, which are: negative control feed (basal feed-non-diabetic) (R1); positive control feed (commercial diabetic food) (R2); basal feed+Clove’s EO (R3); and basal feed+Lemongrass’s EO (R4). Each treatments using 5 rats. Collected data were performances and blood hematology profile. Data were analyzed using one way ANOVA design, the significance of different was tested using Least Significant Different (LSD) test. The result from this research showed that Clove’s and Lemongrass’s EO were significantly affected (P<0.05) on post-induction initial weight (5.41%; 3.30%; and 5.21%) compared to R2 group. It also significantly affected (P<0.05) on total gain (56.66% and 3.30%) and final weight (5.47% and 3.48%) compared to R1 group. Hematology profile’s data showed that both of Clove’s and Lemongrass’s EO did not affect to erythrocyte level, however, Clove’s EO did significantly affect to thrombocyte, hemoglobin and leukocyte level compared to R2 group, consecutively: (25.70%; 87.79%; and 37.59%), while Lemongrass’s EO significantly affected hemoglobin level (51.17%) compared to R2 group. It can be concluded that the addition of Clove’s and Lemongrass’s EO on basal feed could maintain the performance and improve the hematology profile of diabetic rat.
Co-Authors Achmad Fauzi Ahmad Baidlowi Ahmad Baidlowi Ahmad Baidlowi Ari Indrawati Baidlowi, Ahmad Fathur Rohman Haryadi Febri Ariyanti Herawati, Okti Ida Tjahajati Lucky Ronald Runtuwene Wayan Tunas Artama Yutaka Suzuki