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All Journal Jurnal Sain Veteriner BALI MEDICAL JOURNAL (BMJ) Indonesian Journal of Tropical and Infectious Disease agriTECH Indonesian Journal of Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Animal Production : Indonesian Journal of Animal Production Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Proceeding of International Conference Health, Science And Technology (ICOHETECH) Jurnal Kedokteran Hewan Animal Production

Wayan Tunas Artama

Universitas Gadjah Mada

Author-ID : 233968

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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VARIASI GENETIK KAMBING BENGGALA DI KABUPATEN MANGGARAI BARAT BERDASARKAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA Pakpahan, Suhendra; Artama, Wayan Tunas; Widayanti, Rini; Budisatria, I Gede Suparta
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 2 (2018): December 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Genetic Variation of Benggala Goats in West Manggarai Regency Based on Random Amplified Polymorphic DNA Method ABSTRACTIndonesia has several types of local goats that have had an extended period of adaptation to the natural conditions in Indonesia. Goat is one of the most important germplasm in supporting the economy of rural communities. Benggala is a local breed of goat originating from Flores Island, East Nusa Tenggara province and has distinctive characteristics. The RAPD technique has several advantages and has been widely used in studies of the genetic diversity of goats. A total of 50 blood samples of Benggala goats were taken from four sub-districts in West Manggarai Regency. This study was conducted to estimate genetic variations of Benggala goats using OPA-6 and OPA-16 primers. The OPA-6 primer consisted of 0-11 bands, while the OPA-16 primer consisted of 0-7 bands. The total bands produced on the OPA-6 primer from all samples was 456, whilst OPA-16 primer was 314. The lowest genetic similarity between individuals of Benggala goats was 44% from the sample K46. Based on the sample population, the average genetic similarity level was 72%. These results show that the genetic diversity of Benggala goats is low.Keywords: Benggala goat, genetic similarity,genetic variation, RAPD, West Manggarai ABSTRAKIndonesia memiliki beberapa jenis kambing lokal yang memiliki periode adaptasi yang panjang dengan kondisi alam di Indonesia. Kambing merupakan salah satu plasma nutfah yang sangat penting dalam mendukung perekonomian masyarakat pedesaan. Benggala adalah jenis kambing lokal yang berasal dari pulau Flores, propinsi Nusa Tenggara Timur dan kambing Benggala memiliki ciri khas. Teknik RAPD memiliki beberapa keunggulan dan telah banyak digunakan pada studi keragaman genetik kambing. Total 50 sampel darah kambing Benggala yang diambil dari empat kecamatan di Kabupaten Manggarai Barat. Penelitian ini dilakukan untuk menguji variasi genetik kambing Benggala dengan menggunakan primer OPA-6 dan OPA-16. Primer OPA-6 terdiri dari 0-11 band, sedangkan primer OPA-16 terdiri dari 0-7 band. Total jumlah pita yang dihasilkan pada primer OPA-6 dari semua sampel adalah 456, sementara primer OPA-16 adalah 314. Kemiripan genetik terendah antara individu-individu kambing Benggala adalah 44% dari sampel K46. Berdasarkan populasi sampel, tingkat kemiripan genetik rata-rata adalah 72%. Hasil ini menunjukkan bahwa keragaman genetik kambing Benggala tergolong rendah.Kata Kunci: kambing Benggala, kemiripan genetik, Manggarai Barat , RAPD, variasi genetik

SPECIFIC GYRB SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATED FROM SPUTUM OF PULMONARY TUBERCULOSIS PATIENTS IN INDONESIA Mertaniasih, Ni Made; Wiqoyah, Nurul; Kusumaningrum, Deby; Soedarsono, S.; Perwitasari, Agnes Dwi Sis; Artama, Wayan Tunas
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (790.131 KB)

Background: Indonesia have many different geographic areas which could be various on the variant strains of Mycobacterium tuberculosis. The gyrB gene codes GyrB protein as sub unit compound of Gyrase enzyme that functioning in multiplication of bacteria. Detection of gyrB gene could be a marker of active multiplication of viable bacteria in the specimen from patients; and some of the DNA sequence regions were conserved and specific in the strain of Mycobacterium tuberculosis that would be a marker for identification. This research aims to analyze the sequence of gyrB gene of Mycobacterium tuberculosis clinical isolates from sputum of pulmonary TB patients in Indonesia, and determine the specific region. Method: Mycobacterium tuberculosis clinical isolates have been collected from sputum of the patients with pulmonary TB that live in some area in Indonesia. Isolation and identification of Mycobacterium tuberculosis clinical isolates using standard culture method; sequence analysis using PCR-direct sequencing of the part bases region of gyrB. Results: this study revealed that nucleotide sequence on a fragment 764 bases of gyrB gene Mycobacterium tuberculosis strains among clinical isolates almost identically to a wild type strain Mycobacterium tuberculosis H37Rv and subspecies member of Mycobacterium tuberculosis complex (MTBC), with a little difference of SNPs; there are many difference nucleotide sequence with MOTT and Gram positive or negative bacteria, except Corynebacterium diphtheria identically with MTBC. Conclusion: the gyrB sequence in Mycobacterium tuberculosis strains among these clinical isolates from sputum of pulmonary TB patients in Indonesia have the conserved specific DNA region that almost identically with wild type strain H37Rv and MTBC.

Study of Local Herb Potency as Rumen Modifier: Red Ginger (Zingiber Officinale Var. Rubrum) Addition Effect on In Vitro Ruminal Nutrient Digestibility Kurniawati, Asih; Yusiati, Lies Mira; Widodo, Widodo; Artama, Wayan Tunas
ANIMAL PRODUCTION Vol 21, No 1 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Red ginger is herb that commonly used as part of traditional medicine due to its essential oil content. Some essential oil have proven as rumen fermentation modifier. Addition of red ginger in ruminant diet was studied using in vitro gas production technique to evaluate its effect on nutrient digestibility. Red ginger meal was added to meet essential oil level in fermentation medium of 0 mg/l as control, 25, 50, 75 and 100 mg/l. The diet consisted of Penisetum hybride, rice bran, wheat pollard in ratio 60:20:20 DM basis. Feed fermentation was incubated for 24 h at 39Â°C. At the end of incubation data of gas production volume was taken, and residual feed were collected for further nutrient analysis to calculate the digestibility of dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF). Variance analysis was used to analysis collected data, followed by DMRT analysis.Â Addition of red ginger increase total volume of gas production at level 50 mg/L and above. DM and OM digestibility did not affected by red ginger addition whereas CP digestibility were significantly decreased start at level 50 mg/l. In contrast, CF digestibility of treatment level of 50 mg/l significantly higher than control. In conclusion, addition of red ginger correspond to level essential oil of 50 to 100 mg/l improve ruminal nutrient fermentation.

VARIASI GENETIK KAMBING BENGGALA DI KABUPATEN MANGGARAI BARAT BERDASARKAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA Pakpahan, Suhendra; Artama, Wayan Tunas; Widayanti, Rini; Budisatria, I Gede Suparta
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Genetic Variation of Benggala Goats in West Manggarai Regency Based on Random Amplified Polymorphic DNA MethodÃ‚Â ABSTRACTIndonesia has several types of local goats that have had an extended period of adaptation to the natural conditions in Indonesia. Goat is one of the most important germplasm in supporting the economy of rural communities. Benggala is a local breed of goat originating from Flores Island, East Nusa Tenggara province and has distinctive characteristics. The RAPD technique has several advantages and has been widely used in studies of the genetic diversity of goats. A total of 50 blood samples of Benggala goats were taken from four sub-districts in West Manggarai Regency. This study was conducted to estimate genetic variations of Benggala goats using OPA-6 and OPA-16 primers. The OPA-6 primer consisted of 0-11 bands, while the OPA-16 primer consisted of 0-7 bands. The total bands produced on the OPA-6 primer from all samples was 456, whilst OPA-16 primer was 314. The lowest genetic similarity between individuals of Benggala goats was 44% from the sample K46. Based on the sample population, the average genetic similarity level was 72%. These results show that the genetic diversity of Benggala goats is low.Keywords: BenggalaÃ‚Â goat, genetic similarity,genetic variation, RAPD, West ManggaraiÃ‚Â ABSTRAKIndonesia memiliki beberapa jenis kambing lokal yang memiliki periode adaptasi yang panjang dengan kondisi alam di Indonesia. Kambing merupakan salah satu plasma nutfah yang sangat penting dalam mendukung perekonomian masyarakat pedesaan. Benggala adalah jenis kambing lokal yang berasal dari pulau Flores, propinsi Nusa Tenggara Timur dan kambing Benggala memiliki ciri khas. Teknik RAPD memiliki beberapa keunggulan dan telah banyak digunakan pada studi keragaman genetik kambing. Total 50 sampel darah kambing Benggala yang diambil dari empat kecamatan di Kabupaten Manggarai Barat. Penelitian ini dilakukan untuk menguji variasi genetik kambing Benggala dengan menggunakan primer OPA-6 dan OPA-16. Primer OPA-6 terdiri dari 0-11 band, sedangkan primer OPA-16 terdiri dari 0-7 band. Total jumlah pita yang dihasilkan pada primer OPA-6 dari semua sampel adalah 456, sementara primer OPA-16 adalah 314. Kemiripan genetik terendah antara individu-individu kambing Benggala adalah 44% dari sampel K46. Berdasarkan populasi sampel, tingkat kemiripan genetik rata-rata adalah 72%. Hasil ini menunjukkan bahwa keragaman genetik kambing Benggala tergolong rendah.Kata Kunci: kambing Benggala, kemiripan genetik, Manggarai Barat , RAPD, variasi genetik

VARIASI GENETIK KAMBING BENGGALA DI KABUPATEN MANGGARAI BARAT BERDASARKAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA Suhendra Pakpahan; Wayan Tunas Artama; Rini Widayanti; I Gede Suparta Budisatria
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Genetic Variation of Benggala Goats in West Manggarai Regency Based on Random Amplified Polymorphic DNA MethodÂ ABSTRACTIndonesia has several types of local goats that have had an extended period of adaptation to the natural conditions in Indonesia. Goat is one of the most important germplasm in supporting the economy of rural communities. Benggala is a local breed of goat originating from Flores Island, East Nusa Tenggara province and has distinctive characteristics. The RAPD technique has several advantages and has been widely used in studies of the genetic diversity of goats. A total of 50 blood samples of Benggala goats were taken from four sub-districts in West Manggarai Regency. This study was conducted to estimate genetic variations of Benggala goats using OPA-6 and OPA-16 primers. The OPA-6 primer consisted of 0-11 bands, while the OPA-16 primer consisted of 0-7 bands. The total bands produced on the OPA-6 primer from all samples was 456, whilst OPA-16 primer was 314. The lowest genetic similarity between individuals of Benggala goats was 44% from the sample K46. Based on the sample population, the average genetic similarity level was 72%. These results show that the genetic diversity of Benggala goats is low.Keywords: BenggalaÂ goat, genetic similarity,genetic variation, RAPD, West ManggaraiÂ ABSTRAKIndonesia memiliki beberapa jenis kambing lokal yang memiliki periode adaptasi yang panjang dengan kondisi alam di Indonesia. Kambing merupakan salah satu plasma nutfah yang sangat penting dalam mendukung perekonomian masyarakat pedesaan. Benggala adalah jenis kambing lokal yang berasal dari pulau Flores, propinsi Nusa Tenggara Timur dan kambing Benggala memiliki ciri khas. Teknik RAPD memiliki beberapa keunggulan dan telah banyak digunakan pada studi keragaman genetik kambing. Total 50 sampel darah kambing Benggala yang diambil dari empat kecamatan di Kabupaten Manggarai Barat. Penelitian ini dilakukan untuk menguji variasi genetik kambing Benggala dengan menggunakan primer OPA-6 dan OPA-16. Primer OPA-6 terdiri dari 0-11 band, sedangkan primer OPA-16 terdiri dari 0-7 band. Total jumlah pita yang dihasilkan pada primer OPA-6 dari semua sampel adalah 456, sementara primer OPA-16 adalah 314. Kemiripan genetik terendah antara individu-individu kambing Benggala adalah 44% dari sampel K46. Berdasarkan populasi sampel, tingkat kemiripan genetik rata-rata adalah 72%. Hasil ini menunjukkan bahwa keragaman genetik kambing Benggala tergolong rendah.Kata Kunci: kambing Benggala, kemiripan genetik, Manggarai Barat , RAPD, variasi genetik

Peran Lactoferin Susu Kuda sebagai Imunomodulator dalam Respon Imun Humoral Nurliyani Nurliyani; Mochamad Adnan; Wayan Tunas Artama; Zuheid Noor
agriTECH Vol 25, No 2 (2005)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Lactoferrin is a multifunctional defense protein, eg. as immunomodulator , which is known as glycoprotein which has 80 kDa molecullar weight. The aim of this research is to characterize of horse milk lactoferrin as immunomodulator in humoral immune response. Lactoferrin in defiled horse milk, was isolated and purified on Ion Exchange (CM Sephadex C-50) and Gel Filtration (Sephadex G-75) column. Horse milk lactoferrin is administered orally into a group of 6-8 weeks old female Balb/c mice. The dose of lactoferrin were : 0 nig (control); 0,1 mg; 0,5 mg, and 1,0 mg/mice/day in assay period of 28 day. On the r; 4 216, and 286 day, specific IgA and IgG serum of mice were detected with enzyme-linked immunosrbent assay (ELISA). The results showed that IgG and Ig,4 responses of mice that were administered with 0,5 mg or 1,0 mg horse milk lactoferrin, were higher than the control and 0,1 mg lactoferrin (P<0.05). Horse milk lactoferrin apparently induced subclass IgG1 and IgG2b response. It can be concluded that horse milk lactoferrin which has function as immunomodulator in humoral immune response

Adherence Pheno-genotypic of Escherichia coli O157:H7 Isolated from Beef, Feces of Cattle, Chicken and Human I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Dayono
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Generally, adherence of micro-organisms to host cells is the frst step of the colonization to host surfaces.Escherichia coli O157:H7 can colonize to the intestine and induce attaching-effacing (AE) lessions. The capacityof inducing AE lesions is encoded by a pahtogenicity island, the locus of enterocyte effacement (LEE) thatcontains genes involved in generation of attaching and effaching (A/E) lesions. Among which that, the eaegene is encoding intimin, an outer membrane protein that is responsible to intimate attachment to the intestinalepithelial cells. A total of 20 local isolates obtained from human clinically, beef, cattle, chicken, and humannon-clinically were tested to adherence pheno-genotypic of E. coli O157:H7. The eae gene was identifed usingpolymerase chain reaction with a specifc primer i.e AE19 forward and AE20 reverse. To confrm phenotypicof gene, further study was performed by culturing the bacteria in vero cell, followed by Giemsa staining andAcridine Orange Fluorescent staining 3 h and 6 h after incubation, respectivelly. Result of study showed thatthere were 19 out of 20 (95%) isolates identifed positive eae gene. Giemsa staining appeared that the bacteriawith positive eae gene performed a cluster arroud of cell (localized adherence). On the other hand, the negativeeae gene appeared as a diffuse adherence (DA). The study indicated that almost all of E. coli O157:H7 localisolates which was positive eae gene had potency to colonize to the intestine and induce attching-effachinglessions, and also cause cytopahatic effects in intestinal epithelial cell

Isolasi dan identifikasi ekspresi protein rekombinan granule-1 (GRA-1) Toxoplasma gondii isolat lokal Hevi Wihadmadyatami; Rini Widayanti; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 29, No 2 (2011): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Toxoplasmosis is a diseases caused by intracellular protozoa called Toxoplasma gondii.

STATUS KLINIS DAN DETEKSI LipL32 SAPI SEROPOSITIF LEPTOSPIROSIS DI KABUPATEN KULON PROGO Guntari Titik Mulyani; Wayan Tunas Artama; Estu Widodo
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.55930

Leptospirosis is a zoonotic disease caused by Leptospira interrogans. Animals can act as carriers, spread leptospires in urine, and be a source of infection for other animals and humans. In leptospirosis cows can cause abortion, early birth, infertility, decreased milk production and death. The aims of this study was to determine the clinical status and detect the presence of leptospires with Polymerase Chain Reaction (PCR) from urine cows that are expressed as leptospirosis seropositive. A total of 12 cattle seropositive leptospirosis with Microscopic Agglutination Test (MAT) were carried out clinical examinations covering general conditions, pulsus examination, breathing and temperature and organ systems. The urine is collected aseptically, then DNA isolation is carried out using a kit from Genoid. Detection of leptospires in the urine is carried out by detecting the presence of the primary lipoprotein LipL32 making up the Leptospira membrane. The primer was designed with a 21-base forward forward: 5'-TGG ATC TGA TCA ACT ATT ACG-3 ‘containing 38.1% GC with Tm 57.2oC. 22 bases reverse reverse obtained: 5 '-CAC TTC ACC TGG TTT GTA GGT-3' containing GC 45.5% with Tm 62.1oC. Amplification was carried out as many as 40 cycles and continued with electrophoresis to determine the band formed at a wavelength of 506 bp. The results showed that all cows that were positive for leptospirosis with MAT were in a clinically healthy condition. In electrophoresis there are 7 out of 12 positive samples found in the urine leptospira indicated by the formation of a band at 506 bp. From the results of this study it can be concluded that leptospirosis-positive cows do not always show clinical symptoms, but have the potential to excrete leptospires along with urine, so they can act as a source of transmission of leptospirosis to humans, other animals, and the environment.

Lactobacillus Composed Urinary Microbiome of Healthy People and Urinary Tract Infection Patients Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Vol 8 No 4 (2022): Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Buton

Lactobacillus has long been known to reconstruct the microbiome of the urogenital organs of healthy people. However, changes in lactobacillus abundance are known occurring in urogenital tract infections (UTI) patients. It is also known that there are Lactobacillus species that have specific genomic characteristics and nutritional requirements that cause them to turn into pathogens. Understanding population characteristics and taxa composition that reconstruct urine microbiome is very important for the diagnosis and treatment of urogenital infections. This review provides an update information of metagenomic sequencing to identify the urinary microbiome profile and the role of lactobacilli in healthy and UTI patients.

Co-Authors Agnes Dwi Sis Perwitasari, Agnes Dwi Sis Agung Budiyanto Agung Janika Sitasiwi Ahmad Baidlowi Alek Ibrahim Ari Indrawati Asih Kurniawati Atmoko, Bayu Andri Bambang Sumiarto BUDI SETIADI DARYONO Budi Setiadi Dayono Deby Kusumaningrum Edy Dharmana Endah Retnaningrum Estu Widodo Estu Widodo Fitri Nadifah Guntari Titik Mulyani Henricus Roby Cahya Putra Hevi Wihadmadyatami I Gede Suparta Budisatria I Gede Suparta Budisatria I Made Damriyasa I Wayan Suardana Ida Ayu Pasti Apsari Juwari J Lies Mira Yusiati Lucky Ronald Runtuwene Mochamad Adnan Nesti, Dela Ria Ni Made Mertaniasih Nina Difla Muflikhah Nurliyani Nurliyani Nurul Wiqoyah, Nurul Pakpahan, Suhendra Pakpahan, Suhendra Rini Widayanti Rini Widayanti Rini Widayanti S. Soedarsono Sri Hartati Sugiwinarsih S Suhendra Pakpahan Sumartono s Widodo Widodo Widya Asmara Yutaka Suzuki Zuheid Noor

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