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All Journal Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia bionature
Dirayah R Husain
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Efek Ekstrak Kasar Cacing Tanah Pheretima sp. terhadap Morfologi Sel Bakteri Salmonella enteritidis Sahribulan, Sahribulan; Rauf H, Dirayah; Hasyim, Zohra
bionature Vol 19, No 2 (2018): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.188 KB) | DOI: 10.35580/bionature.v19i2.9733

Abstract

Abtract. This study was aimed at studying changes in the morphology of Salmonella enteritidis bacterial cells after the crude extract of Pheretima sp. using a scanning electron microscope (SEM). Earthworm extraction uses 96% ethanol, and binds to the appropriate bacteria in the exponential phase with extract concentrations of 5% and 10%. Observations using a microscope scanner were carried out after agreeing to a 6-hour, 12-hour, and 24-hour extract. Obtained from the results showing the crude extract of Pheretima sp. to changes in cell morphology of Salmonella enteritidis bacteria. Salmonella enteritidis bacterial cells are damaged consisting of: formed holes in the surface of bacterial cells, bumps on the surface of bacterial cells, shrinkage of bacterial cells, elongation of bacterial cells, and increase in bacterial cells. In addition, as well as longer contributions and free time, incubation, after being added, crude extract of Pheretima sp. against Salmonella enteritidis bacteria, the greater the damage caused. However, 5% extract concentration is the optimal concentration in the effect of damage to the morphology of the cells of Salmonella enteritidis bacteria.Keywords: Coarse Extract, Earthworms, Pheretima sp., Morphological Cells, Salmonella enteritidis, Scanning Electron Microscope (SEM).
BIOCHEMICAL COMPOSITION AND MODES OF SUBSTRATE UPTAKE TO ALKANE-GROWN MARINE BACTERIUM Pseudomonas nautica STRAIN 617 Dirayah R Husain
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 12 No. 2 (2005): Desember 2005
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.773 KB)

Abstract

Komposisi protein, karbohidrat dan lemak dari kultur (sel dan supernatan) bakteri laut Pseudomonas nautica strain 617dipengaruhi substrat pertumbuhan (acetate atau eicosane). Bentuk transport substrat ke permukaan sel: adherence, emulsifikasi dan solubilisasi dipengaruhi oleh komposisi biokimiawi yang diproduksi bakteri. Peningkatan konsentrasi senyawa ekstraseluler nampak selama pertumbuhan bakteri pada substrat eicosane. Aktifitas solubilisasi, emulsifikasi dan adherence berubah pada saat terjadi perubahan komposisibiokimiawi ekstraseluler. Diperoleh prosentase biodegradasi eicosane sebesar 59% setelah kultur berumur3.5 hari.Kata kunci: komposisi biokimiawi, transport substrat, biodegradasi, bakteri laut, n-alkana.
Co-Authors Sahribulan, Sahribulan Zohra Hasyim, Zohra