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Composition and diversity of arbuscular mycorrhizal fungi spore associated with different land-use types in tropical gold mine Faisal Danu Tuheteru; H Husna; A Albasri; Asrianti Arif; Kartini Kramadibrata; Geofrey Soka
Journal of Degraded and Mining Lands Management Vol 8, No 1 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2020.081.2503

Abstract

Understanding the composition and diversity of arbuscular mycorrhizal fungi (AMF) is imperative for potentially enhancing their ecological role in different terrestrial ecosystems. Land use can have substantial effects on AMF species composition and diversity, but such effects have been explored less in tropical landscapes. In this study, we assessed the effects of disturbances on AMF species richness, observed the potential development of AMF types to produce mycorrhizal biofertilizer bioinoculants.  This study was conducted identifying and selecting AMFs was for the purpose of managing post-mining land in Bombana District, Southeast Sulawesi, Indonesia.  AMF spores collected from the field and trap culture were directly isolated and morphologically identified. A total of 15 AMF species were identified, including 11 species from field samples and 9 species from trap cultures. We noted that five AMF species were unique to field conditions and 5 AMF species were uniquely isolated from trap culture.  It appears that Glomeraceae family contributed the highest number of species in all land-use types.  Glomus sp. 1 was the most frequent species found in all land-use types. The Simpson’s index, Shannon index and evenness ranged from 1.60 ± 0.51 to 2.40 ± 0.40; 0.41 ± 0.17 to 0.62 ± 0.17; 0.25 ± 0.10 to 0.39 ± 0.11, respectively. In this study, we found three new records of AMF species including Entrophospora colombiana, Sclerocystis microcarpa and Glomus coronatum for Indonesia, i.e. from this study, it is clear that different land-use types affected AMF spore composition and species diversity.  All AMF species found in this study were then applied to the land to improve land quality.
Trapping Cultures For Arbuscula Mycorrhyz Fungi (AMF) Spores Traping From Post-Mine Asphalt Soils Using Different Host Plants Tuheteru, Faisal Danu; Husna; Asrianti Arif; Albasri; Rahmat; Wiwin Rahmawati Nurdin
Journal of Tropical Mycorrhiza Vol. 3 No. 2 (2024): October
Publisher : Asosiasi Mikoriza Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/jtm.v3i2.67

Abstract

Spore trapping Culture has the advantage of producing AMF. inoculum production and higher A.M.F. colonization efficiency. This research aims to determine suitable hostnames for AMF trapping. This research was conducted at the Plastic House of the Indonesian Mycorrhizal Association (AMI.) Southeast Sulawesi Branch, UHO Old Campus, and the UHO. Forestry and Environmental Science Laboratory Unit lasted 3 months from May – June 2023. This research used a factorial, completely randomized design method with two factors: the first factor is a type of location, including L10 LM10, and the second factor is the type of host plant, including Pueraria javanica and Sorhum bicolar. The results showed that the interaction of the host plant and location had no significant effect on the number of spores and AMF colonization. Pueraria Javanica significantly increased the number of spores for both L10 and LM10, with an average number of spores of 84. Seven dominant types of A.M.F. from the Glomeraceae family were found
Success of Siompu Orange Plant Shoot Cuttings With Natural Growth Regulators and Arbuscular Mycorrhzal Fungi (AMF) Application Husna, Husna; Tuheteru, Faisal; Wiwin Rahmawati Nurdin; Asrianti Arif; Nais Mawarsyah Lauti
Journal of Tropical Mycorrhiza Vol. 4 No. 1 (2025): April
Publisher : Asosiasi Mikoriza Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/jtm.v4i1.83

Abstract

Siompu tangerine (Citrus nobilis Lour.) is an endemic plant from Siompu Island, This plant is rare and slow to bear fruit due to generally being cultivated generatively. This study aims to determine the effect of natural growth regulators combined with Arbuscular Mycorrhizal Fungi on the success of Siompu tangerine shoot cuttings. This research was conducted in the plastic house of the Indonesian Mycorrhizal Association (AMI) Southeast Sulawesi Branch Universitas Halu Oleo (UHO) and the Kendari Forestry and Environmental Sciences Laboratory Unit, which lasted for 5 months from September to February 2025. This study used a Randomized Block Design consisting of 9 treatments, namely control (a), aloe vera solution (b), shallot solution (c), AMF Inoculum of Kaimbulawa Village (d), AMF Inoculum of Lapara (e), Aloe vera solution + AMF Inoculum of Kaimbulawa Village (f), Aloe vera solution + AMF Inoculum of Lapara (g), Shallot solution + AMF Inoculum of Kaimbulawa Village (h) and Shallot solution + AMF Inoculum of Lapara ( i ) each treatment consisted of three replications and each replication consisted of five plants so that the total plants used were 135 plants. The results showed that the treatment of natural growth regulator combined with AMF had not provided significant results on the Percentage of living cuttings, the percentage of cuttings that sprouted, and the number of leaves. In the Kaimbulawa AMF treatment, it can increase the rate of rooted cuttings, and the 100% natural shallot PGR treatment combined with Lapara FMA increases root length and the percentage of AMF colonization