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All Journal Jurnal Biologi Universitas Andalas Journal of Aceh Physics Society Jurnal Biologi Tropis
Maya Sari
Universitas Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Physics

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The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro Maya Sari; Mayta Novaliza Isda
Jurnal Biologi Universitas Andalas Vol 9, No 1 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.1.8-17.2021

Abstract

The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting.
Biosynthesis of ZnO Micro-Nanoflower with Ananas comosus Peel Extract Maya Sari; Yolanda Rati; Tetty Marta Linda; Yanuar Hamzah; Ari Sulistyo Rini
Journal of Aceh Physics Society Volume 10, Number 4, October 2021
Publisher : PSI-Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jacps.v10i4.18951

Abstract

 Abstrak. Dalam rangka mengurangi pemakaian bahan kimia berbahaya, kini telah mulai dikembangkan penggunaan bahan alami pada proses pembentukan nanopartikel. Pada penelitian ini, biosintesis nanopartikel ZnO dilakukan menggunakan ekstrak kulit Ananas comosus sebagai agen capping sekaligus agen pereduksi. Seng nitrat digunakan sebagai prekursor dari ion seng, sedangkan kulit nanas dibuat menjadi ekstrak untuk dimanfaatkan kandungan antioksidannya. Sampel ZnO dipelajari sifat fisisnya dari hasil karakterisasi X-ray diffraction (XRD), scanning electron microscopy (SEM) dan spektroskopi UV-Vis. Berdasarkan pola XRD, nanopartikel ZnO memberikan fasa kristal heksagonal wurtzite dengan ukuran kristal 14 nm. Morfologi SEM masing-masing sampel didapatkan berbentuk bunga atau micro-nanoflower dengan ukuran diameter rata-rata 510 nm dan 560 nm untuk sampel 0,01 M dan 0,025 M. Hasil spektrum absorbansi UV-Vis menunjukkan peningkatan puncak penyerapan cahaya dengan penambahan konsentrasi seng nitrat. Berdasarkan informasi sifat fisis ini, sampel ZnO berpotensi diaplikasikan sebagai material fotokatalis.Abstract. In order to reduce the use of hazardous chemicals, the use of natural ingredients has now been developed in the process of forming nanoparticles. In this study, biosynthesis of ZnO nanoparticles was carried out using Ananas comosus peel extract as capping agent and reducing agent. Zinc nitrate was used as a precursor to zinc ion. The physical properties of ZnO samples were studied from the characterization result of scanning electron microscopy (SEM), UV-Vis spectroscopy, and X-ray diffraction (XRD). The SEM morphology of each different sample was obtained in the form of micro-nanoflower with an average diameter  of 510 nm and 560 nm for 0.01 M and 0.025 M samples, respectively. The UV-Vis absorbance spectrum results showed an increase in the light absorption peak as  zinc nitrate concentration increased. According to the XRD pattern, the ZnO nanoparticles possessed an hexagonal wurtzite crystal phase with a crystal size of 14 nm. Based on this information on physical properties, the ZnO sample has the potential to be applied as a photocatalyst material.
Phytochemical Contents of Underutilized Edible Plant from Riau Province, Ridan (Nephelium maingayi Hiern – Sapindaceae) Nery Sofiyanti; Mayta Novaliza Isda; Fitmawati Fitmawati; Asih Rahayu Ajeng Agesti; Ikhwan Taufik; Maya Sari; syafroni Pranata
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2567

Abstract

Ridan (Nephelium maingayi) is one of Nephelium (Sapindaceae) member distributed in Riau Province. This underutilized edible plant is commonly found as wild species, and poses three different fruit morphologies (oval, asymmetric and rounded shaped fruit). The phytochemical contents of these fruit species had not been reported. This study aimed to identify the phytochemical contents of fruit parts (peel, aril and seed) of three different fruit shapes of N. maingayi (oval, asymmetric and rounded shaped fruit) from Riau Province. A total of six secondary metabolite groups were tested (alkaloid, terpenoid, steroid, flavonoid, saponin dan tannin), using qualitative analysis based on the presence of precipitation (alkaloid), the change of mixture color (steroid, terpenoid, flavonoid and tannin) and soapy foaming substance for saponin. The result showed that peels of Ridan (N. maingayi) gave the highest phytochemical contents (alkaloid, terpenoid, flavonoid, saponin and tannin.). Terpenoid and saponin were found in peel, aril and seed of three different fruit shapes. This study concluded that the phytochemical contents of three fruit shapes of Ridan (N. maingayi) varies among the different shapes of fruit. This result of this study provide the first information of bioactive profile of N. maingayi form Riau Province.
Co-Authors Asih Rahayu Ajeng Agesti Fitmawati Fitmawati Ikhwan Taufik Mayta Novaliza Isda Nery Sofiyanti Rati, Yolanda Rini, Ari Sulistyo syafroni Pranata Tetty Marta Linda Yanuar Hamzah