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Journal : JURNAL BIOLOGI INDONESIA

Resistensi Beberapa Isolat Bakteri terhadap Logam Berat (Hg, As, Cd, Ni, Pt dan Se) Imamuddin, Hartati
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i2.3484

Abstract

ABSTRACTStudy of Some Bacteria to Heavy Metal Resistent (Hg, As, Cd, Ni, Pt and Se). The study was conducted to evaluate the resistance of some bacteria toward heavy metal (Hg, As, Cd, Ni, Pt and Se) by monitoring growth inhibition in Minimal Basal Medium (MBM) and Nutrient Broth (NB). Prior to growth inhibition study, pre selection of heavy metal resistance was determined by disk blanks methods. The results showed that AT 01, AT 03 and AT 11 were found to be heavy metal-resistent, however Pseudomonas sp. was a heavy metal-sensitive. The study also showed that AT 01 grew better in NB than MBM.Key words: Resistance, heavy metal, Pseudomonas sp.
Pertumbuhan Bakteri Hasil Isolasi dari Tanah Perkebunan yang Tumbuh pada Media Mengandung Pestisida Propoksur dan Karbaril Imamuddin, Hartati; Laili, Nur; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.636 KB) | DOI: 10.14203/jbi.v8i2.3054

Abstract

Growth Capacity of Soil Bacteria Isolates Obtained from Estate Area from Media Containing Propoxur and Carbaryl. Isolation actions have been carried out with three soil samples derived from soil estate of pine apple, sugar cane, and banana in Lampung area, Southern Sumatra. The isolates were grown in the selected media containing carbaryl insecticide, and herbicide of diuron and bromocyl. The highest and vigorous growth isolates have gathered from threedifferent soil samples, and subsequently named GGPC, GM, and NTF isolates. Furthermore, those isolates were studied through the growth rate in the media containing propoxur and carbaryl in the nutrient rich liquid (NB) and compared to limited mineral (MM). All of the isolates grew faster against pesticides in the liquid medium of NB compared to MM. High correlated value among parameters nurtured as due to bacterial population and those processes were not affected by media composition. The GM isolate showed similar pattern during propoxur decrease along with incubation even though the rate of pesticide elimination from the media was higher in the rich nutrient liquid media (NB) compared to MM.Keywords: microbe isolate, pesticide, propoxur, carbaryl
Oksidasi Nitrit Oleh Bakteri Heterotrofik Pada Kondisi Aerobik Agustiyani, Dwi; Kayadoe, Ruly Marthina; Imamuddin, Hartati
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3164

Abstract

ABSTRACTNitrite Oxidation by Heterotrophic Bacteria Under Aerobic Condition. The nitrite transformingactivities of heterotrophic bacteria from isolates from agriculture soil, Lampung were studiedunder aerobic conditions. Among the 9 bacterial isolates tested, almost all are reported haveability to consume of nitrite, but none of the bacterial isolates formed significant nitrate in themedium. NOB H1 (Bacillus licheniformis), is denitrification-negative, consumed 16. 4 mg/L ofnitrites with the accumulation of 4.45 mg/L nitrates. While, NOB H8 (Pseudomonas sp.) isdenitrification-positive, consumed 49. 64 mg/L of nitrite with the accumulation of 3.34 mg/Lnitrates. Nitrite oxidations of both isolates NOB H1 and NOB H8 took place during stationeryphase to the dead phase. Growth pattern of both isolates NOB H1 and NOB H8 were sigmoidwith generation time of 1.69 and 2.19 hour, respectivelyKey words: heterotrophicbacteria; nitrite oxidation; denitrification
Karakter Pertumbuhan dan Aktivitas Nitrifikasi Kultur Mikroba N-Sw Agustiyani, Dwi; Imamuddin, Hartati; Haryanto, Tanto
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.815 KB) | DOI: 10.14203/jbi.v5i1.3211

Abstract

ABSTRACTThe growth character and nitrification activity of nitrifying cultures (N-Sw). Theculture of nitrifiers (N-Sw) was obtained from acclimated sludge of sawit palm oil industrywastewater. The growth and nitrification activity of those cultures were investigated.The result shows that the growth and nitrification activity attained optimum at pH 7-8,and temperature of 30 0C. The culture of nitrifiers was still growing at pH 5, but thenitrification activity was not detected. The growth of nitrifiers and the nitrification activitywas inhibited at 40 0C. The ammonium conversion rate reached 0,088-0,090 mg NNH4+/L/hour/g biomass. The rate of ammonium conversion in the bioreactor increasedto 0.630 mg N-NH4+/L/hour/g biomass as the pH maintained at 7.5-8 and dissolvedoxygen at 3-4 mg/l O2.Key words: nitrifying culture, ammonium, ammonium convertion rate
RESISTENSI BEBERAPA ISOLAT BAKTERI TERHADAP LOGAM BERAT (HG, AS, CD, NI, PT DAN SE) Imamuddin, Hartati
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i2.3484

Abstract

ABSTRACTStudy of Some Bacteria to Heavy Metal Resistent (Hg, As, Cd, Ni, Pt and Se). The study was conducted to evaluate the resistance of some bacteria toward heavy metal (Hg, As, Cd, Ni, Pt and Se) by monitoring growth inhibition in Minimal Basal Medium (MBM) and Nutrient Broth (NB). Prior to growth inhibition study, pre selection of heavy metal resistance was determined by disk blanks methods. The results showed that AT 01, AT 03 and AT 11 were found to be heavy metal-resistent, however Pseudomonas sp. was a heavy metal-sensitive. The study also showed that AT 01 grew better in NB than MBM.Key words: Resistance, heavy metal, Pseudomonas sp.
OKSIDASI NITRIT OLEH BAKTERI HETEROTROFIK PADA KONDISI AEROBIK Agustiyani, Dwi; Kayadoe, Ruly Marthina; Imamuddin, Hartati
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3164

Abstract

ABSTRACTNitrite Oxidation by Heterotrophic Bacteria Under Aerobic Condition. The nitrite transformingactivities of heterotrophic bacteria from isolates from agriculture soil, Lampung were studiedunder aerobic conditions. Among the 9 bacterial isolates tested, almost all are reported haveability to consume of nitrite, but none of the bacterial isolates formed significant nitrate in themedium. NOB H1 (Bacillus licheniformis), is denitrification-negative, consumed 16. 4 mg/L ofnitrites with the accumulation of 4.45 mg/L nitrates. While, NOB H8 (Pseudomonas sp.) isdenitrification-positive, consumed 49. 64 mg/L of nitrite with the accumulation of 3.34 mg/Lnitrates. Nitrite oxidations of both isolates NOB H1 and NOB H8 took place during stationeryphase to the dead phase. Growth pattern of both isolates NOB H1 and NOB H8 were sigmoidwith generation time of 1.69 and 2.19 hour, respectivelyKey words: heterotrophicbacteria; nitrite oxidation; denitrification
PERTUMBUHAN BAKTERI HASIL ISOLASI DARI TANAH PERKEBUNAN YANG TUMBUH PADA MEDIA MENGANDUNG PESTISIDA PROPOKSUR DAN KARBARIL Imamuddin, Hartati; Laili, Nur; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3054

Abstract

Growth Capacity of Soil Bacteria Isolates Obtained from Estate Area from Media Containing Propoxur and Carbaryl. Isolation actions have been carried out with three soil samples derived from soil estate of pine apple, sugar cane, and banana in Lampung area, Southern Sumatra. The isolates were grown in the selected media containing carbaryl insecticide, and herbicide of diuron and bromocyl. The highest and vigorous growth isolates have gathered from threedifferent soil samples, and subsequently named GGPC, GM, and NTF isolates. Furthermore, those isolates were studied through the growth rate in the media containing propoxur and carbaryl in the nutrient rich liquid (NB) and compared to limited mineral (MM). All of the isolates grew faster against pesticides in the liquid medium of NB compared to MM. High correlated value among parameters nurtured as due to bacterial population and those processes were not affected by media composition. The GM isolate showed similar pattern during propoxur decrease along with incubation even though the rate of pesticide elimination from the media was higher in the rich nutrient liquid media (NB) compared to MM.Keywords: microbe isolate, pesticide, propoxur, carbaryl
KARAKTER PERTUMBUHAN DAN AKTIVITAS NITRIFIKASI KULTUR MIKROBA N-SW Agustiyani, Dwi; Imamuddin, Hartati; Haryanto, Tanto
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3211

Abstract

ABSTRACTThe growth character and nitrification activity of nitrifying cultures (N-Sw). Theculture of nitrifiers (N-Sw) was obtained from acclimated sludge of sawit palm oil industrywastewater. The growth and nitrification activity of those cultures were investigated.The result shows that the growth and nitrification activity attained optimum at pH 7-8,and temperature of 30 0C. The culture of nitrifiers was still growing at pH 5, but thenitrification activity was not detected. The growth of nitrifiers and the nitrification activitywas inhibited at 40 0C. The ammonium conversion rate reached 0,088-0,090 mg NNH4+/L/hour/g biomass. The rate of ammonium conversion in the bioreactor increasedto 0.630 mg N-NH4+/L/hour/g biomass as the pH maintained at 7.5-8 and dissolvedoxygen at 3-4 mg/l O2.Key words: nitrifying culture, ammonium, ammonium convertion rate