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KARAKTERISTIK AMILOGRAFI TEPUNG SORGUM FERMENTASI DAN APLIKASINYA PADA PRODUK CAKE DAN COOKIES SORGUM Raden Haryo Bimo Setiarto; Nunuk Widhyastuti; Iwan Saskiawan
Jurnal Dinamika Penelitian Industri Vol 28, No 1 (2017): JURNAL DINAMIKA PENELITIAN INDUSTRI
Publisher : Balai Riset dan Standardisasi Industri Palembang

This study aims to investigate the characteristics of fermented sorghum flour amylograph and organoleptic test of the product cake and cookies from fermented sorghum flour. Production of sorghum flour was made with four variations of treatments, ie: control (without fermentation), solid fermentation, liquid fermentation, mixture fermentation (solid and liquid). Amylograph profile analyzes the four samples of sorghum flour was conducted with Viscograph Brabender. In the manufacture of cookies and cake, wheat flour can be substituted with fermented sorghum flour on percentage (0%, 25%, 50%, 75%, 100%). Organoleptic test of the product cake and cookies sorghum were calculated using hedonic test. The results of amylograph analysis indicated that sorghum flour with liquid fermentation treatment has the best profile of gelatinization because it had resistance, high stability about heating and it also had a good setback viscosity. Overall, cookies and cake was made from mixture of sorghum flour and wheat flour in the ratio 1 : 1 (substitution of wheat flour 50%) were well received by the panelists.

Produksi sari pepaya (Carica papaya) fermentasi sebagai minuman probiotik antihiperkolesterolemia Raden Haryo Bimo Setiarto; Nunuk Widhyastuti; Nandani Dwi Octavia; Herson Cahaya Himawan
Jurnal Litbang Industri Vol 8, No 1 (2018)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Probiotic beverages can be used as antihipercholesterolemia therapy. One potential ingredient for probiotic drinks is papaya (Carica papaya) which can be fermented using lactic acid bacteria. The aim of this research is to get the right mixture of lactic acid bacteria culture formula to produce probiotic papaya juice which can decrease total cholesterol level in white rats (Rattus norvegicus) of Sprague Dawley strain. The papaya juice is fermented using a mixed culture formulation of different lactic acid bacteria, comprising A (Lactobacillus bulgaricus: Lactobacillus acidophilus: Streptococcus thermophilus); B (Lactobacillus plantarum: Lactobacillus acidophilus: Streptococcus thermophilus); C (Lactobacillus casei: Lactobacillus acidophilus: Streptococcus thermophilus). The results showed that the best formula of probiotic papaya juice mixed using A mixture culture based on pH parameter, total lactic acid and total lactic acid bacteria. After the total cholesterol test, the results obtained that probiotic papaya juice beverage can reduce total cholesterol levels with a decrease percentage of 17.51%. Probiotic papaya juice beverage can be applied to antihipercholesterolemia therapy in humans at doses of 55.56 ml each day.AbstrakMinuman probiotik dapat digunakan sebagai terapi antihiperkolesterolemia. Salah satu bahan potensial untuk minuman probiotik yaitu pepaya (Carica papaya) yang dapat difermentasi menggunakan bakteri asam laktat. Tujuan penelitian ini adalah memperoleh formula kultur campuran bakteri asam laktat yang tepat untuk memproduksi minuman sari pepaya probiotik yang mampu menurunkan kadar kolesterol total pada tikus putih (Rattus norvegicus) jantan galur Sprague Dawley. Sari pepaya difermentasi menggunakan formulasi kultur campuran bakteri asam laktat yang berbeda, terdiri dari A (Lactobacillus bulgaricus: Lactobacillus acidophilus: Streptococcus thermophilus); B (Lactobacillus plantarum: Lactobacillus acidophilus: Streptococcus thermophilus); C (Lactobacillus casei: Lactobacillus acidophilus: Streptococcus thermophilus). Hasil penelitian menunjukkan bahwa formula terbaik minuman sari pepaya probiotik menggunakan kultur campuran A berdasarkan parameter pH, total asam laktat dan total bakteri asam laktat. Setelah dilakukan uji kolesterol total, maka didapatkan hasil bahwa minuman sari pepaya probiotik dapat menurunkan kadar kolesterol total dengan persentase penurunan sebesar 17,51%. Minuman sari pepaya probiotik dapat diaplikasikan untuk terapi antihiperkolesterolemia pada manusia dengan dosis sebesar 55,56 ml per hari.

PENINGKATAN KADAR PATI RESISTEN TIPE III TEPUNG SINGKONG TERMODIFIKASI MELALUI FERMENTASI DAN PEMANASAN BERTEKANAN-(Improvement Level of Resistant Starch Type III on Modified Cassava Flour Using Fermentation and Autoclaving-Cooling) Raden Haryo Bimo Setiarto; Nunuk Widhyastuti; Arumsyah Sumariyadi
Biopropal Industri Vol 9, No 1 (2018)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Modified Cassava flour (Mocaf) is a product derived from cassava flour that uses principles of cassava cell modification through fermentation for 12-72 hours. This study aims to improve the levels of resistant starch in cassava flour using lactic acid bacteria fermentation and autoclaving-cooling. Cassava slices fermented with mixed cultures of lactic acid bacteria (Lactobacillus plantarum B-307:Leuconostoc mesenteroides SU-LS 67=1:1) for 18 hours at 37oC. The fermented cassava then autoclaved (121oC, 15 min) and cooled (4oC, 24 hours) for 1-3 cycles. Cassava slices was dried (70oC, 16 hours), grounded and sieved (80 mesh) to obtain modified cassava flour. Combination of autoclaving-cooling and fermentation could increase resistant starch level of Mocaf. Fermentation with 2 cycles of autoclaving-cooling (FAC-2S) produced the highest resistant starch content (12.51%) compared to other treatments. This value was around 4.5-fold higher than the control (2.81%). Increased levels of resistant starch could contribute to decrease the digestibility of Mocaf.Keywords: autoclaving-cooling, fermentation, lactic acid bacteria, modified cassava flour, resistant starch ABSTRAKTepung singkong termodifikasi (Mocaf) merupakan produk turunan dari tepung singkong yang menggunakan prinsip modifikasi sel singkong secara fermentasi selama 12-72 jam. Penelitian ini bertujuan meningkatkan kadar pati resisten tepung singkong melalui fermentasi bakteri asam laktat dan pemanasan bertekanan-pendinginan. Irisan singkong difermentasi dengan kultur campuran bakteri asam laktat (Lactobacillus plantarum B-307:Leuconostoc mesenteroides SU-LS 67 = 1:1) selama 18 jam pada suhu 37oC. Irisan singkong fermentasi selanjutnya di autoklaf (121oC, 15 menit) dan didinginkan (4oC, 24 jam) untuk 1-3 siklus. Irisan singkong kemudian dikeringkan (70oC, 16 jam), digiling dan diayak (80 mesh) untuk mendapatkan tepung singkong termodifikasi. Kombinasi pemanasan bertekanan-pendinginan dengan fermentasi mampu meningkatkan kadar pati resisten pada tepung singkong termodifikasi. Perlakuan fermentasi dengan 2 siklus pemanasan bertekanan-pendinginan (FAC-2S) menghasilkan kadar pati resisten tertinggi (12,51%) dibanding perlakuan lainnya dan meningkatkan kadar pati resisten sebesar 4,5 kali lipat dibandingkan perlakuan kontrol (2,81%). Peningkatan kadar pati resisten menyebabkan terjadinya penurunan daya cerna pada tepung singkong termodifikasi.Kata kunci: bakteri asam laktat, fermentasi, pati resisten, pemanasan bertekanan-pendinginan, tepung singkong termodifikasi

Pengaruh Fermentasi Bakteri Asam Laktat dan Siklus Pemanasan Bertekanan-Pendinginan Terhadap Kadar Pati Resisten Tepung Ubi Jalar Ungu (Ipomea Batatas Var Ayamurasaki) Termodifikasi Raden Haryo Bimo Setiarto; Nunuk Widhyastuti
Warta Industri Hasil Pertanian Vol 34, No 1 (2017)
Publisher : Balai Besar Industri Agro

Penelitian ini bertujuan menganalisis pengaruh fermentasi kultur campuran bakteri asam laktat dan siklus pemanasan bertekanan–pendinginan terhadap kadar pati resisten tepung ubi jalar ungu termodifikasi. Irisan ubi jalar ungu difermentasi dengan kultur campuran bakteri asam laktat (Lactobacillus plantarum B-307: Leuconostoc mesenteroides SU-LS 67) (1:1) (vol/vol) selama 24 jam pada suhu 370C. Irisan ubi jalar ungu fermentasi selanjutnya diautoklaf (1210C, 15 menit) dan didinginkan (40C, 24 jam), perlakuan dilakukan untuk satu, dua dan tiga siklus. Irisan ubi jalar ungu kemudian dikeringkan (800C, 18 jam), digiling dan diayak (80 mesh) untuk mendapatkan tepung ubi jalar ungu modifikasi. Kombinasi pemanasan bertekanan-pendinginan dengan fermentasi mampu meningkatkan kadar pati resisten pada tepung ubi jalar ungu modifikasi. Semakin banyak jumlah siklus pemanasan bertekanan-pendinginan yang diaplikasikan dapat meningkatkan kadar pati resisten secara signifikan. Perlakuan fermentasi dengan 1 siklus pemanasan bertekanan-pendinginan (FAC-1S) menghasilkan kadar pati resisten tertinggi (11,26%) dibanding perlakuan lainnya dan meningkatkan kadar pati resisten sebesar 5,34 kali lipat dibandingkan perlakuan kontrol (2,11%). Peningkatan kadar pati resisten menyebabkan terjadinya penurunan daya cerna pada tepung ubi jalar ungu modifikasi.

Produksi sari pepaya (Carica papaya) fermentasi sebagai minuman probiotik antihiperkolesterolemia Raden Haryo Bimo Setiarto; Nunuk Widhyastuti; Nandani Dwi Octavia; Herson Cahaya Himawan
Jurnal Litbang Industri Vol 8, No 1 (2018)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Probiotic beverages can be used as antihipercholesterolemia therapy. One potential ingredient for probiotic drinks is papaya (Carica papaya) which can be fermented using lactic acid bacteria. The aim of this research is to get the right mixture of lactic acid bacteria culture formula to produce probiotic papaya juice which can decrease total cholesterol level in white rats (Rattus norvegicus) of Sprague Dawley strain. The papaya juice is fermented using a mixed culture formulation of different lactic acid bacteria, comprising A (Lactobacillus bulgaricus: Lactobacillus acidophilus: Streptococcus thermophilus); B (Lactobacillus plantarum: Lactobacillus acidophilus: Streptococcus thermophilus); C (Lactobacillus casei: Lactobacillus acidophilus: Streptococcus thermophilus). The results showed that the best formula of probiotic papaya juice mixed using A mixture culture based on pH parameter, total lactic acid and total lactic acid bacteria. After the total cholesterol test, the results obtained that probiotic papaya juice beverage can reduce total cholesterol levels with a decrease percentage of 17.51%. Probiotic papaya juice beverage can be applied to antihipercholesterolemia therapy in humans at doses of 55.56 ml each day.AbstrakMinuman probiotik dapat digunakan sebagai terapi antihiperkolesterolemia. Salah satu bahan potensial untuk minuman probiotik yaitu pepaya (Carica papaya) yang dapat difermentasi menggunakan bakteri asam laktat. Tujuan penelitian ini adalah memperoleh formula kultur campuran bakteri asam laktat yang tepat untuk memproduksi minuman sari pepaya probiotik yang mampu menurunkan kadar kolesterol total pada tikus putih (Rattus norvegicus) jantan galur Sprague Dawley. Sari pepaya difermentasi menggunakan formulasi kultur campuran bakteri asam laktat yang berbeda, terdiri dari A (Lactobacillus bulgaricus: Lactobacillus acidophilus: Streptococcus thermophilus); B (Lactobacillus plantarum: Lactobacillus acidophilus: Streptococcus thermophilus); C (Lactobacillus casei: Lactobacillus acidophilus: Streptococcus thermophilus). Hasil penelitian menunjukkan bahwa formula terbaik minuman sari pepaya probiotik menggunakan kultur campuran A berdasarkan parameter pH, total asam laktat dan total bakteri asam laktat. Setelah dilakukan uji kolesterol total, maka didapatkan hasil bahwa minuman sari pepaya probiotik dapat menurunkan kadar kolesterol total dengan persentase penurunan sebesar 17,51%. Minuman sari pepaya probiotik dapat diaplikasikan untuk terapi antihiperkolesterolemia pada manusia dengan dosis sebesar 55,56 ml per hari.

Pengaruh Siklus Pemanasan Bertekanan-Pendinginan terhadap Komposisi Kimia dan Kualitas Biologi Tepung Campolay (Pouteria campheciana) Raden Haryo Bimo Setiarto; Lia Amalia; Yusdianti Febriani; Tiana Fitrilia; Nunuk Widhyastuti
Jurnal Riset Teknologi Industri Vol.13. No.1 JUNI 2019
Publisher : Balai Riset dan Standardisasi Industri Samarinda

Campolay (Pouteria campechiana) has complete content of fiber, starch, calcium, phosphorus, carotenoid, thiamin, riboflavin, niacin, and vitamin C. The presence of this ingredient makes Campolay potentially an alternative functional food ingredient. Campolay processing in flour products was done to extend shelf life, improve functional properties, and diversify products, thus expanding use. Resistant starch (RS) is a starch that is not able to be digested by digestive enzyme and resistant to gastric acid that it is able to reach colon to be fermented by probiotic bacteria. RS is classified as insoluble fiber source and is able to decrease the glycemic index and cholesterol, preventing the colon cancer, and helping the absorption of mineral. The aim of this study is to produce the resistant starch of campolay flour through autoclaving-cooling cycle treatment. Slices of campolay were autoclaved (121oC, 15 minutes) and cooled (4oC, 24 hours). The treatment was done for one, two and three cycles. Then, the campolay slices were dried (70oC, 7 hours), milled and sifted (100 mesh) to obtain modified campolay flour. The modified Campolay flour was analyzed for amylose content, total starch, reducing sugar content, Rapid Digestible Starch (RDS), Slowly Digestible Starch (SDS) and Resistant Starch / RS levels, starch digestibility with three replications. The treatment of 1 cycle of autoclaving-cooling (AC-1S) resulted the highest levels of resistant starch (36.23% bk) when compared to other treatments. It could increase the resistance of starch levels by 2.4-fold compared to the control treatment (15, 31% bk).

Pengaruh Starter Bakteri Asam Laktat dan Penambahan Tepung Talas Termodifikasi terhadap Kualitas Yogurt Sinbiotik Raden Haryo Bimo Setiarto; Nunuk Widhyastuti
Jurnal Riset Teknologi Industri Vol 11 No.1 JUNI 2017
Publisher : Balai Riset dan Standardisasi Industri Samarinda

Yogurt was the one of functional food product which contains probiotic bacteria to improve the balance of intestinal microflora. The functional value of yogurt can be increased by the addition of modified taro flour to stimulate the growth of probiotic bacteria in the digestive tract. This study aimed to determine the effect of variation lactic acid bacteria starter and fortified of modified taro flour for the quality of taro yogurt synbiotic. This research used statistical randomized block design. The parameters which used to determine the quality of yogurt in this study were pH value, total acid content, the viability of lactic acid bacteria and organoleptic tests. The results showed that the best combination starter to produce yogurt were starter Lactobacillus bulgaricus + Lactobacillus achidophillus + Streptococcus thermophillus with a pH value of 4, total acid content 1.32%, total lactic acid bacteria 6.93 log cfu / ml, and total organoleptic test by a value of 4.5. Meanwhile, the best formulation was added to modify taro flour (TTM) 0.5 % (w/v) to produce taro yogurt synbiotic with pH value 4.5, total acid content 1.44%, total lactic acid bacteria (LAB) 7.01 log cfu / ml, and total organoleptic test 4.215. Variation of lactic acid bacteria starter and fortification of modified taro flour was not given significant effect for pH, total lactic acid content and organoleptic quality (taste, texture, colour, smell) of synbiotic yogurt. Based on microbiology and organoleptic standard, qualities of taro yogurt synbiotic products stay awake during the storage period of 4 weeks at cold temperatures.Keywords: product quality, lactic acid bacteria starter, prebiotic taro flour, taro yogurt synbioticABSTRAKYogurt merupakan salah satu produk pangan fungsional yang mengandung bakteri probiotik untuk memperbaiki keseimbangan mikroflora usus. Nilai fungsional yogurt dapat ditingkatkan dengan penambahan tepung talas termodifikasi untuk menstimulasi pertumbuhan bakteri probiotik dalam saluran pencernaan. Penelitian ini bertujuan untuk mengetahui pengaruh variasi starter bakteri asam laktat dan penambahan tepung talas termodifikasi terhadap kualitas yogurt talas sinbiotik. Metode penelitian ini menggunakan rancangan acak kelompok. Parameter yang diamati pada penelitian ini adalah nilai pH, total asam tertitrasi, viabilitas bakteri asam laktat dan uji organoleptik. Hasil penelitian menunjukan bahwa variasi starter terbaik adalah yogurt yang dibuat dari Lactobacillus bulgaricus + Lactobacillus achidophillus + Streptococcus thermophillus dengan nilai pH 4, total asam tertitrasi 1,32%, total bakteri asam laktat 6,93 log cfu/ml, dan total uji organoleptik dengan nilai 4,5. Sementara itu penambahan tepung talas termodifikasi (TTM) terbaik untuk produksi yogurt talas sinbiotik adalah sebesar 0,5% (b/v) dengan nilai pH 4,5, total asam tertitrasi 1,44%, total bakteri asam laktat (BAL) 7,01 log cfu/ml, dan total uji organoleptik 4,215. Perlakuan variasi starter kultur bakteri asam laktat dan penambahan tepung talas termodifikasi tidak memberikan pengaruh yang signifikan terhadap perubahan nilai pH, kadar total asam tertitrasi dan kualitas organoleptik (rasa, tekstur, warna, aroma) dari yogurt sinbiotik. Secara mikrobiologis dan organoleptik kualitas produk yogurt talas simbiotik tetap terjaga selama masa penyimpanan 4 minggu pada suhu dingin.Kata Kunci: Kualitas produk, starter bakteri asam laktat, tepung talas prebiotik, yogurt talas sinbiotik

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