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All Journal Jurnal Penelitian Saintek Jurnal Ilmu Kimia Universitas Brawijaya Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology Science and Technology Indonesia Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Journal of Research on Community Engagement

Ellya Indahyanti

Department Of Chemistry , Faculty Of Mathematics And Natural Sciences, Brawijaya University .

Author-ID : 286554

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Health Professions Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences

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KINETIKA REAKSI FERMENTASI GLUKOSA HASIL HIDROLISIS PATI BIJI DURIAN MENJADI ETANOL Salsabila, Usyqi; Mardiana, Diah; Indahyanti, Ellya
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.344 KB)

Ethanol can be produced by glucose fermentation process using Saccharomyces cerevisiae. The potential source of glucose is starch of durian seed. This research studies about determine the kinetics model of ethanol fermentation of glucose and to identify the physical properties of ethanol produced. Glucose fermentation using Saccharomyces cerevisiae for 72 hours at various initial concentrations of 20, 40, 60 and 80 ppm has been conducted. After ethanol produced was reacted with potassium dichromate using Conway crucible. It was then analyzed by UV-Vis spectrophotometer at 605 nm. The physical properties of ethanol determined were density and refraction index. The result showed that the kinetics model of ethanol fermentation obtained was accordance with = -0,0011 + 0,0002 equation. As for Michaelis-Menten (Km) constant was 5,5 ppm-1 and maximum velocity (Vmax) 5000 ppm.hour-1. In addition, its physical properties i.e. density and refractive index were in the range of 0,9767-0,9809 g/mL and 1,3347-1,3365 respectively.

PENGARUH pH DAN TEMPERATUR TERHADAP KESTABILAN AKTIVITAS XILANASE DARI Trichoderma viride Kusuma, Sicilia; Sutrisno, Sutrisno; Indahyanti, Ellya
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Xylanase is an enzyme that can hydrolyze xylan into reducing sugar xylose and xilo-oligosaccharides. The research was carried out to determine the effect of pH and temperature on stability of xylanase activity of T. viride . Its effects were observed by measuring the residual activity after the enzyme xylanase was incubated for 9 hours at pH variation (4, 5, 6) and the variation of temperature (50, 60, 70 oC ). Enzyme activity is said to be stable if the remaining activity is greater than or equal to 50%. Xylanase activity was determined by measuring the levels of reducing sugars produced during the enzymatic reaction, by spectrophotometry using DNS reagent. The results showed that the xylanase activity remained stable after the enzyme was incubated for 9 hours at pH 4, 5, 6 and at temperatures of 50, 60, 70 oC. The stability of xylanase activity is highest when xylanase was incubated at pH 5 and temperature 60 ° C.Key words: enzyme stability , pH, temperature , Trichoderma viride, xylanase.

PEMURNIAN ETANOL SECARA MIKROFILTRASI MENGGUNAKAN MEMBRAN SELULOSA ESTER Pratama, Agung Yanuar; Mardiana, Diah; Indahyanti, Ellya
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Studi tentang pemurnian etanol secara mikrofiltrasi menggunakan membran selulosa ester telah dilakukan. Tujuan penelitian ini adalah untuk mengetahui apakah metode mikrofiltrasi dapat digunakan untuk meningkatkan kemurnian etanol, dan mengetahui pengaruh waktu filtrasi terhadap tingkat kemurnian etanol. Membran yang digunakan adalah mix-selulosa ester yang telah dikompaksi dengan sampel yang digunakan etanol 96%. Karakter membran yang digunakan dievaluasi berdasarkan hasil SEM dan spektrofotometri FT-IR. Mikrofiltrasi dilakukan hingga 120 menit pada tekanan 1 atm. Analisis hasil filtrasi didasarkan pada massa jenis dan indeks bias. Hasil penelitian menunjukkan bahwa waktu filtrasi berpengaruh terhadap kemampuan membran menahan etanol, meskipun membran selulosa ester kurang sesuai untuk meningkatkan kemurnian etanol secara mikrofiltrasi. Kata Kunci : Etanol, Mikrofiltrasi, Selulosa Ester

PENGARUH HIDROFILISITAS MEMBRAN TERHADAP PENINGKATAN KEMURNIAN ETANOL SECARA DESTILASI Sitanggang, Fetty Alvionita; Mardiana, Diah; Indahyanti, Ellya
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 2 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Metoda destilasi membran dimungkinkan digunakan untuk meningkatkan kemurnian etanol. Dua jenis membran yang digunakan pada kajian ini adalah membran selulosa ester dan membran poliamida. Tujuan penelitian ini adalah untuk mengetahui pengaruh hidrofilisitas membran dan waktu destilasi terhadap kemurnian etanol. Destilasi yang dikombinasikan dengan membran dilakukan pada temperatur 80oC dengan variasi waktu destilasi 1 sampai 3 jam. Hasil destilasi, baik destilat maupun residu, dianalisis berdasarkan nilai indeks bias dan massa jenisnya. Sebagai pendukung juga telah dilakukan analisis sifat termal dan derajat swelling membran. Hasil analisis termal menunjukkan bahwa membran selulosa ester tidak sesuai untuk pemurnian secara destilasi, Membran poliamida yang lebih bersifat hidrofilik dibandingkan membran selulosa ester lebih mampu menahan air, meskipun belum dapat digunakan untuk meningkatkan kemurnian etanol secara destilasi. Semakin lama waktu destilasi, kemampuan membran poliamida untuk melewatkan etanol semakin tinggi.Kata kunci: etanol, destilasi, membran, hidrofilik

KINETIKA REAKSI HIDROLISIS PATI BIJI DURIAN (Durio zibethinus Murr.) MENJADI GLUKOSA DENGAN VARIASI TEMPERATUR DAN WAKTU Anugrahini, Sarah Fitria Agung; Ismuyanto, Bambang; Indahyanti, Ellya
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh temperatur dan waktu pada proses hidrolisis pati biji durian (Durio zibethinus Murr.) menjadi glukosa serta untuk menentukan kinetika reaksi pada proses hidrolisis tersebut. Proses hidrolisis dilakukan dengan menggunakan katalis HCl 3M. Variasi temperatur yang digunakan yaitu 50 oC dan 70 oC dengan variasi waktu sebesar 30, 60, 90, 120, 150 dan 180 menit. Kadar glukosa larutan hidrolisat kemudian dianalisis menggunakan reagen DNS. Berdasarkan hasil penelitian, dapat diketahui bahwa temperatur dan waktu hidrolisis mempengaruhi kadar glukosa. Semakin tinggi temperatur dan semakin lama waktu hidrolisis maka kadar glukosa larutan hidrolisat semakin meningkat. Kadar glukosa larutan hidrolisat kemudian digunakan untuk menentukan kinetika reaksi hidrolisis. Kinetika reaksi hidrolisis pati biji durian (Durio zibethinus Murr.) menjadi glukosa dapat dinyatakan dengan persamaan: k = 3,593 x 106 . e-10153,812/T M.menit -1 Katakunci: biji durian, glukosa, hidrolisis, kinetika, pati ABSTRACT This experiment aim to find out the influence of temperature and time on hydrolysis of durian (Durio zibethinus Murr.) seed starch become glucose and to determine the reaction kinetics of hydrolysis process. Hydrolysis process was conducted by using HCl 3M as catalyst. The temperature varied from 50 oC and 70 oC, while time variation varied from 30, 60, 90, 120, 150 and 180 minutes. Glucose levels of hydrolysate solution was analyzed using DNS reagent. This experiment resulted that temperature and hydrolysis time affected the glucose levels. Increasing of temperature and hydrolysis time produced higher glucose levels of hydrolysate solution. The glucose levels of hydrolysate solution used to determine the reaction kinetics. Reaction kinetics of durian seed hydrolysis become glucose stated with equation: k = 3,593 x 106 . e-10153,812/T M.minutes -1 Keywords: durian seed, glucose, hydrolysis, kinetics, starch

ENZYMATIC SYNTHESIS OF CELLULOSE PROPIONATE AND ITS POTENCY AS RAW MATERIAL FOR MEMBRANE Anna Roosdiana; Diah Mardiana; Ellya Indahyanti; Dyah Ayu Oktavianie
Journal of Environmental Engineering and Sustainable Technology Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

ABSTRACT Modification of bacterial cellulose through enzymatic esterification between cellulose and propionic acid has been carried out to produce cellulose propionate as raw material of dialysis membrane. The research aimed to characterize of cellulose propionate and assess its potency as raw material for membrane. The esterification was performed for 8 hours at 50 ⁰C with cellulose and propionic acid in mass ratio of 1:5. The physical chemical properties of resulted cellulose propionate were determined for functional group using FTIR, crystallinity index by XRD, swelling index by gravimetric method, specific gravity, maximum pore size diameter and membrane thickness. The cellulose propionate showed peak absorbance at wave number of 1743 cm-1 from C=O absorption and 1108 cm-1 and 1037 cm-1 from C-O absorption with degree of crystallinity of 61.56% and density of 1.39 g/cm3. Cellulose propionate membrane has maximum poressize of 2.25 ± 0.04 µm and thickness of 0.029 ±0.001mm, the swelling index of 153%. Diffusion equilibrium of uric acid was 3 hours at average diffusion rate of 1.48ppm/h.Keywords : Bacterial cellulose, cellulose propionate, characteristic, diffusion rate

OPTIMIZATION OF BISULFIT SALT CONCENTRATION ON COCONUT QUALITY CONTROL OF COCONUT Ellya Indahyanti; Budi Kamulyan; Bambang Ismuyanto
Jurnal Penelitian Saintek Vol 19, No 1: April 2014
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Bisulphite salt is an additive that usualy added into foodstuff, it was used in order to take a preservation effects. In this research, it was added into coconut sap. The aim of this research was to study the effects of sodium bisulphite adding for increasing coconut sap quality, including pH, reducing sugar and sucrose content. In addition, the rate of sucrose hydrolysis with and without bisulphite have been compared. The volumetric method, i.e Lane-Eynon procedure was used for determining the sugar contents while the hydrolysis rates were measured by polarimetry. The results showed that bisulphite added into coconut sap could repress sucrose hydrolysis.

Enzymatic Cellulose Palmitate Synthesis Using Immobilized Lipase Anna Roosdiana; Diah Mardiana; Ellya Indahyanti; Dyah Ayu Oktavianie
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Bacterial cellulose can be modified by esterification using palmitic acid and Mucor miehei lipase as catalyst. The purpose of this research was to determine the optimum conditions of esterification reaction of cellulose and palmitic acid . The esterification reaction was carried out at the time variation of 6, 12, 18, 24 and 30 hours and the mass ratio of cellulose: palmitic acid (1: 11: 2, 1: 3, 1: 4, 1: 5,1:6) at 50 °C. The cellulose palmitate was examined its physical and chemical properties by using FTIR spectrophotometer, XRD, bubble point test and saponification apparatus. The results showed that the optimum reaction time of esterification reaction of cellulose and palmitic acid occurred within 24 hours and the mass ratio of cellulose: palmitic acid was 1: 3 resulting in DS of 0.376 with swelling index of 187 %, crystallinity index of 61.95%, and Φ porous of 2.40 μm. Identification of functional groups using FTIR spectrophotometer showed that C=O ester group was observed at 1737.74 cm-1 and strengthened by the appearance of C-O ester peak at 1280 cm-1. The conclusion of this study is reaction time and reactant ratio influence significantly the DS of cellulose ester.

Okra Mucilage Extract as A Co-Surfactant Increased the Curcumin Nanoemulsion Stability and Encapsulation Efficiency El Fajriyah Aulia Putri; Ellya Indahyanti; Diah Mardiana; Maria Lucia A.D Lestari; Zubaidah Ningsih
Science and Technology Indonesia Vol. 8 No. 3 (2023): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2023.8.3.509-515

Curcumin has various bio-functional properties; however, curcumin poor bioavailability reduces its efficacy. Nanoemulsion delivery system is an alternative method improving curcumin bioavailability in which surfactant and oil used, play an important role in determining nanoemulsion properties. Several studies on curcumin nanoemulsions apply synthetic surfactants which can be harmful if they are added excessively. This study aims to use a natural emulsifying agent, namely okra mucilage extract (OME), and determine its effectiveness as co surfactant. OME is safe to use as an emulsifying agent because it is natural, harmless, safe, biodegradable and eco-friendly. Liquid-liquid and microwave extraction methods were used to obtain OME which was further identified using Fourier Transfer Infrared Spectroscopy (FTIR). Meanwhile, sonication method was used to produce curcumin nano-emulsion (CurN). The particle size and polydispersity index of curcumin nano-emulsion were measured using Particle Size Analyzer (PSA) with Dynamic Light Scattering (DLS) technique, while the morphology of the nanoemulsion was observed using a Digital Imaging Microscope and Confocal Laser Scanning Microscope (CLSM). The results showed that the addition of 0.0160 g OME at a ratio of 1:5 (OME: Tween 80) in the preparation of 5 mL of CurN was able to reduce the particle size and polydispersity index from 740.80 ± 9.70 nm to 289.20 ± 2.23 and 0.340 ± 0.005 to 0.165 ± 0.008 respectively. OME increased the encapsulation efficiency from 77.93 ± 6.59% to 87.17 ± 1.12% which was confirmed by the augmentation of the fluorescence intensity of curcumin from 192.82 to 388.55. The addition of OME also maintained the stability of the CurN up to 14 days of storage at 4°C.

Pendampingan Sertifikasi Halal Jalur Sehati (Self-Declare) Bagi Pelaku Usaha Mikro Kecil di Kecamatan Pujon Anna Safitri, M.Sc., Ph.D (Scopus ID : 57189988164; Sinta ID : 257342); Sri Wardhani; Ellya Indahyanti; Layta Dinira; Sovia Rosalin; Yenny Kornitasari
TRI DHARMA MANDIRI: Diseminasi dan Hilirisasi Riset kepada Masyarakat (Jurnal Pengabdian kepada Masyarakat) Vol 3, No 1 (2023)
Publisher : JTRIDHARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2023.003.01.1

Menguatnya industri halal di Indonesia berimplikasi positif pada peningkatan tingkat perekonomian masyarakat. Sertifikasi halal selain sebagai bentuk perlindungan terhadap jaminan kehalalan produk, juga memberikan nilai tambah yang berimplikasi positif terhadap volume produksi dan penjualan. Sertifikasi halal adalah suatu proses untuk memperoleh sertifikat halal melalui beberapa tahap pemeriksaan untuk membuktikan bahwa bahan, proses produksi, dan sistem jaminan halal dapat memenuhi standar Badan Penyelenggara Jaminan Produk Halal. Tujuan dari kegiatan pengabdian kepada masyarakat ini adalah pendampingan pendaftaran Nomor Induk Berusaha (NIB) bagi Usaha Mikro dan Kecil (UMK); pendampingan tentang peraturan jaminan produk halal bagi industri makanan dan minuman UMK; serta pendampingan pengajuan sertifikasi halal bagi UMK. Metode pelaksanaan kegiatan yaitu melalui sosialisasi, pendampingan pengisian persyaratan, dan pengajuan sertifikasi produk halal. Hasil kegiatan adalah pemahaman pelaku usaha tentang jaminan produk halal bagi pelaku UMK di sektor pangan, pendaftaran sertifikasi halal bagi pelaku usaha, dan sertifikat halal bagi UMK yang telah turun sebanyak 10 sertifikat halal.

Co-Authors A.A. Ketut Agung Cahyawan W Agung Yanuar Pratama Anna Roosdiana Anna Roosdiana Anugrahini, Sarah Fitria Agung Bambang Ismuyanto Bambang Ismuyanto Budi Kamulyan Budi Kamulyan Diah Mardiana Diah Mardiana Diah Mardiana Dyah Ayu Oktavianie Dyah Ayu Oktavianie El Fajriyah Aulia Putri Fetty Alvionita Sitanggang Ika Oktavia Wulandari Layta Dinira Maria Lucia A.D Lestari Rosalin, Sovia Safitri, Anna Safitri, M.Sc., Ph.D (Scopus ID : 57189988164 Sicilia Kusuma Sovia Rosalin Sri Wardhani Usyqi Salsabila Yenny Kornitasari Yenny Kornitasari, Yenny Zubaidah Ningsih

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