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Journal : Medula

Bioaktifitas Antibakteri Ekstrak Etanol Teripang Pasir (Holothuria scabra) terhadap Pertumbuhan Klebsiella pneumoniae Secara In Vitro Fifit Ervitha; Sulastrianah Sulastrianah; Indria Hafizah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 3, No 1 (2015)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.561 KB) | DOI: 10.46496/medula.v3i1.2538

Abstract

Holothuria scabra is  one of the biota that commonly found in Southeast Sulawesi waters and traditionally use as a source food and medicine by local tribe. They also harvesting this species for exported to several countrywith high demand.  Even this species have commmercially beneficience, but there is lack of publication about the bioactivity, especially H. Scabra derived from southeast Sulawesi. This study aims to determine secunder metabolite that contain in the extractand to examine antibacterial bioactivity of H. scabra ethanol extract againstKlebsiellapneumoniae by defining the Minimum Inhibitory Consentration. The method using an experimental with post test onlycontrol group design. The extraction is using maseration method and the extract then  diluted and divided intoten concentration 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,78%, 0,39%, and 0,19%. The secunder metabolite was determined by using TLC and the MIC was determined by using dilution method. All consentration.As the result, TLC test showing positif result for alcaloid and triterpenoid and the nutrient broth starting to show no turbidity in concetration 0,78%. The conclusion of this study is ethanol extract of H. Scabra contain alkaloid and triterpenoid as secunder metabolite and the MIC was in 0,78% concentration.Keywords: Holothuria scabra, Klebsiella pneumoniae,  MIC, alkaloid, terpenoid
PERBEDAAN KEJADIAN INFEKSI CACING ANTARA PETUGAS PENGANGKUT SAMPAH YANG MENGGUNAAN ALAT PELINDUNG DIRI DENGAN PETUGAS PENGANGKUT SAMPAH YANG TIDAK MENGGUNAKAN ALAT PELINDUNG DIRI Lezdyana Nur Islami; Sulastrianah Sulastrianah; Wa Ode Sitti Asfiah Udu
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 2, No 1 (2014)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.979 KB) | DOI: 10.46496/medula.v2i1.242

Abstract

Soil  Transmitted  Helminth  (STH)  infection  globally  spread  around  the  worlds.  Incidence  of  helminth infection  more  common  in  childrens,  but  adult  with  special  job  like  garbage  workers  have  same  risk  with  that childrens. The aim of this study is to determine differences between usage of personal protective equipment with the helminth  infection  among garbage  workers that  completely  used  personal  protective  equipement  and  that  not completely used . The method of this study is an observational with cross sectional design. Population in this study are garbage workers at Sanitary Service Wakatobi Regency. Sampling method using total sampling with 59 samples. The data about usage of personal protective equipement have done by observation and faecal examination by direct slide. The differences analysed statistically using Chi-Square test. As a result, it is obtained that among the workers that  not completely using  personal  protective  equipment,  there  27  (60%) samples  are infected  and 18  (40%) samples  are  not  infected.  Among  the  workers  that completely using  personal  protective  equipment, there  is  4 (28,6%) samples are infected and 10 (71,4%) samples are not infected (p = 0,04). The conclusion of this study is there was relation between usage of personal protective equipment with helminth infection in garbage workers. Key Words: Helminth infection, garbage workers, personal protective equipment