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Sernita Sernita
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Uji Kepekaan Kulit Batang Ceremai (Phyllanthus acidus L.) terhadap Pertumbuhan Eschericia coli. Sernita Sernita
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 3, No 2 (2016): Biodiversity in Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.332 KB) | DOI: 10.33772/biowallacea.v3i2.3233

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui kepekaan kulit batang ceremai (phyllanthus acidus L.) dan untuk mengukur diameter zona hambat kulit batang ceremai  terhadap pertumbuhan Escherichia coli ATCC 35218. Sampel dalam penelitian ini adalah kulit batang ceremai yang diekstraksi dengan metode maserasi menggunakan etanol 96%. Dibuat pengenceran dengan konsentrasi 20%, 40%, 60%, 80% dan 100%. Pengujian kepekaan menggunakan media NA dengan metode cawan kertas. Biakan bakteri dari media NB dicampur dengan media agar, kemudian cawan kertas diletakkan di atas media agar. Diinkubasi pada suhu 37oC selama 48 jam. Diukur zona hambat menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa kulit batang ceremai konsentrasi 20% tidak dapat menghambat pertumbuhan bakteri Escherichia coli. Pada konsentrasi 40% rata-rata diameter zona hambat 4,03 mm. Pada konsentrasi 60% rata-rata diameter zona hambat 4,36 mm. Pada konsentrasi 80% rata-rata diameter zona hambat 5,56 mm. Dan pada konsentrasi 100% dapat menghambat pertumbuhan bakteri Escherichia coli secara optimal dengan rata-rata diameter zona hambat 7,03 mm. Kata Kunci : Escherichia coli, ceremai, Nutrient Agar ABSTRACTThis study aims to determine the sensitivity of the bark ceremai (Phyllanthus acidus L.) and to measure the diameter of inhibition zone ceremai bark on the growth of Escherichia coli ATCC 35218. The sample in this study is the bark ceremai extracted by maceration method using ethanol 96%. Created dilution with a concentration of 20%, 40%, 60%, 80% and 100%. NA sensitivity testing using media with paper plate method. NB bacterial culture media mixed with agar medium, then the paper cup is placed on an agar medium. Incubated at 37 ° C for 48 hours. Inhibition zone was measured using a caliper. The results show that the research is conducted bark ceremai concentration of 20% could not inhibit the growth of bacteria Escherichia coli. At a concentration of 40% average inhibition zone diameter of 4.03 mm. At a concentration of 60% average inhibition zone diameter of 4.36 mm. At a concentration of 80% average inhibition zone diameter of 5.56 mm. And at 100% concentration to inhibit the growth of bacteria Escherichia coli optimally with an average diameter of 7.03 mm zone of inhibition. Keywords : Escherichia coli, ceremai, Nutrient Agar
DETEKSI PENYAKIT HEPATITIS-B PADA IBU HAMIL DI PUSKESMAS ABELI KOTA KENDARI Susanti Susanti; Sernita Sernita; Firdayanti Firdayanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 4, No 1 (2017): Biosains & Technology in Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.43 KB) | DOI: 10.33772/biowallacea.v4i1.5852

Abstract

ABSTRAKPenyakit Hepatitis B merupakan peradangan atau infeksi pada sel-sel hati yang disebabkan oleh virus Hepatitis B.  Penularan virus Hepatitis B melalui darah atau cairan tubuh yang mengandung virus Hepatitis B.  Penyakit Hepatitis B dapat dideteksi salah satunya dengan pemeriksaan HBsAg (Hepatitis B Surface Antigen) yang merupakan antigen permukaan dari Virus Hepatitis B. Ibu hamil yang terinfeksi virus Hepatitis B di Indonesia berkisar antara 1-5 %. Infeksi virus Hepatitis B pada ibu hamil dapat mengakibatkan hepatitis fulminan dan meningkatkan mortalitas pada ibu dan bayi. Data yang diperoleh di Puskesmas Abeli Kota Kendari, ibu hamil yang datang berkunjung ke puskesmas Abeli pada bulan Januari-Maret 2017 sebanyak 133 orang, dan tidak terdapat data pemeriksaan Hepatitis B pada ibu Hamil. Tujuan penelitian ini adalah untuk mendeteksi penyakit hepatitis B pada ibu Hamil di Puskesmas Abeli kota Kendari. Jenis penelitian yang digunakan adalah deskriptif observasional, dengan metode cross sectional dan pengambilan data secara Accidental sampling, sampel diperoleh dari pasien ibu hamil yang datang ke Puskesmas Abeli selama penelitian dilakukan. Jumlah sampel penelitian yang dilakukan pemeriksaan Hepatitis B pada ibu Hamil di Puskesmas Abeli sebanyak 25 orang. Hasil penelitian diperoleh sampel positif Hepatitis B sebanyak 1 orang (4%) dan sampel negative Hepatitis B sebanyak 24 orang (96%).  Kesimpulan hasil deteksi Hepatitis B pada ibu Hamil di Puskesmas Abeli kota Kendari menunjukkan terdapat hasil positif Hepatitis B pada ibu Hamil sebanyak 1 orang (4%) dan hasil negative Hepatitis B pada ibu Hamil sebanyak 24 orang (96%). Kata Kunci : Hepatitis-B, Ibu Hamil, Deteksi ABSTRACTHepatitis B is an inflammation or infection of liver cells caused by Hepatitis B virus. Hepatitis B virus transmission through blood or body fluids containing Hepatitis B virus. Hepatitis B disease can be detected by HBsAg examination (Hepatitis B Surface Antigen) which is the surface antigen of Hepatitis B Virus. Pregnant women infected with Hepatitis B virus in Indonesia ranges from 1-5%. Hepatitis B viral infection in pregnant women can lead to fulminant hepatitis and increase maternal and infant mortality. Data obtained at Puskesmas Abeli Kendari, pregnant women who come to Abeli health center in January-March 2017 as many as 133 people, and there is no data of hepatitis B examination in pregnant women. The purpose of this study was to detect hepatitis B disease in Pregnant women at Abeli Puskesmas Kendari. The type of research used is descriptive observasional, with cross sectional method and data collection by Accidental sampling, sample obtained from pregnant woman patient coming to Puskesmas Abeli during research done. The number of samples of research conducted Hepatitis B examination in Pregnant Women at Puskesmas Abeli as many as 25 people. The result of the study was Hepatitis B positive 1 (4%) and Hepatitis B negative sample 24 (96%). The conclusion of result of Hepatitis B detection in pregnant mother at Abeli Kendari health center showed positive result of Hepatitis B in pregnant mother as much as 1 person (4%) and negative result of Hepatitis B in pregnant mother counted 24 people (96%). Keywords: Hepatitis B, Pregnant Women, Detection
UJI KEPEKAAN KULIT BATANG CEREMAI (Phyllanthus acidus L.) TERHADAP PERTUMBUHAN Escherichia coli Sernita Sernita; Anggriani Fusvita; Andri Lina Sumarni
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 3, No 2 (2016): Biodiversity in Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.332 KB) | DOI: 10.33772/biowallacea.v3i2.5851

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui kepekaan kulit batang ceremai (phyllanthus acidus L.) dan untuk mengukur diameter zona hambat kulit batang ceremai  terhadap pertumbuhan Escherichia coli ATCC 35218. Sampel dalam penelitian ini adalah kulit batang ceremai yang diekstraksi dengan metode maserasi menggunakan etanol 96%. Dibuat pengenceran dengan konsentrasi 20%, 40%, 60%, 80% dan 100%. Pengujian kepekaan menggunakan media NA dengan metode cawan kertas. Biakan bakteri dari media NB dicampur dengan media agar, kemudian cawan kertas diletakkan di atas media agar. Diinkubasi pada suhu 37oC selama 48 jam. Diukur zona hambat menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa kulit batang ceremai konsentrasi 20% tidak dapat menghambat pertumbuhan bakteri Escherichia coli. Pada konsentrasi 40% rata-rata diameter zona hambat 4,03 mm. Pada konsentrasi 60% rata-rata diameter zona hambat 4,36 mm. Pada konsentrasi 80% rata-rata diameter zona hambat 5,56 mm. Dan pada konsentrasi 100% dapat menghambat pertumbuhan bakteri Escherichia coli secara optimal dengan rata-rata diameter zona hambat 7,03 mm. Kata Kunci : Escherichia coli, ceremai, Nutrient Agar ABSTRACTThis study aims to determine the sensitivity of the bark ceremai (Phyllanthus acidus L.) and to measure the diameter of inhibition zone ceremai bark on the growth of Escherichia coli ATCC 35218. The sample in this study is the bark ceremai extracted by maceration method using ethanol 96%. Created dilution with a concentration of 20%, 40%, 60%, 80% and 100%. NA sensitivity testing using media with paper plate method. NB bacterial culture media mixed with agar medium, then the paper cup is placed on an agar medium. Incubated at 37 ° C for 48 hours. Inhibition zone was measured using a caliper. The results show that the research is conducted bark ceremai concentration of 20% could not inhibit the growth of bacteria Escherichia coli. At a concentration of 40% average inhibition zone diameter of 4.03 mm. At a concentration of 60% average inhibition zone diameter of 4.36 mm. At a concentration of 80% average inhibition zone diameter of 5.56 mm. And at 100% concentration to inhibit the growth of bacteria Escherichia coli optimally with an average diameter of 7.03 mm zone of inhibition. Keywords : Escherichia coli, ceremai, Nutrient Agar
Co-Authors Andri Lina Sumarni Anggriani Fusvita Firdayanti Firdayanti Susanti Susanti