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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal

W. Widodo

Unknown Affiliation

Author-ID : 2873774

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Human Origin Lactobacillus casei Isolated from Indonesian Infants Demonstrating Potential Characteristics as Probiotics in vitro W. Widodo; Tiyas Tono Taufiq; Ety Aryati; Asih Kurniawati; Widya Asmara
Indonesian Journal of Biotechnology Vol 17, No 1 (2012)
Publisher : Universitas Gadjah Mada

The aim of this experiment was to isolate and identify Lactic Acid Bacteria (LAB) from infant faecesand subsequent evaluation of its potential probiotics. LAB was isolated from faeces of infants who consumedbreast milk as the only source of diet on L-cysteine-supplemented MRS Agar, and incubated on 37oC for 48hours. Colonies grew on this media were then identifi ed based on morphological, physiological and molecularapproaches. Morphological and physiological identifi cations based on Gram staining, shape, motility, sporeformation, catalase, CO2 and NH3 production, and the ability to grow on temperature at 10oC and 45oC.Molecular identifi cation based on the amplifi cation of 16S rRNA gene. The potential application of selectedisolates for probiotics was evaluated based on the ability to grow on media with low pH and the additionof 0.5% bile salts, the ability to inhibit the growth of pathogenic Bacillus cereus and Eschericia coli, and in vitroadherence ability. On the basis of morphological, physiological and molecular analysis of 16S rRNA gene, itwas concluded that the selected isolate 1AF was a strain of Lactobacillus casei. Evaluation of probiotic in vitro showed that 60.4% of cells were resistant to pH 2.0 for 90 minutes. Survival of isolate 1AF after growing at0.5% bile salts was 70.8%. The selected isolate 1AF showed the ability to inhibit the growth of Eschericia coli and Bacillus cereus with inhibitory zone of 12.00±1,00 and 15.33±1.53 mm, respectively. In vitro study on theadherence value of isolate to solid plate was found at 46.5%. It is concluded that Lactobacillus casei isolate 1AFis a potential candidate as probiotics and subject to further in vivo evaluation.

Identification of Pediococcus Strains Isolated from Feces of Indonesian Infants With in vitro Capability to Consume Prebiotic Inulin and to Adhere on Mucus W. Widodo; Nosa Septiana Anindita; Tiyas Tono Taufiq; Tutik Dwi Wahyuningsih
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

The aim of this experiment was to identify isolates obtained from feces of Indonesian infants and to evaluate their capability as probiotics. Identification of isolates was carried out based on morphology, physiology and biochemical identifications, and molecular identification based on 16S rRNA sequence. Morphological and physiological identification was carried out based on Gram staining, shape, motility, spore formation and catalase production. Biochemical identifications based on production of CO2 and NH3 from glucose, the ability to grow on different temperature (10 and 45°C) and pH (4.4 and 9.6), and different salt concentration (6.5 and 18%). Probiotics capability of isolates was assayed on the ability to grow on low pH (pH 2.0), on different bile salts concentration (0.3; 0.5; 1.0 and 1.5%), the capacity to grow on media with inulin as the only carbon source, and in vitro adhesion ability on porcine mucin. Morphological, physiological and biochemical identification suggest that all of isolates belong to lactic acid bacteria. Further molecular identification of five isolates showedthat isolates AA, BE and BK were strains of Pediococcus acidilactici (similarity 99%), while isolate AP and AG were strains of Lactobacillus casei (similarity 99-100%). Probiotic assays showed that more than 80% of cells of Pediococcus acidilactici isolates AA, BE and BK were viable after grown on pH 2.0 for 90 min, and around 80% of cells from the same isolates were survived on media supplemented with bile salt 1.5% for 2 h. All of isolates had high adhesion capacity as seen by more than 75% of cells attached on pig gastric mucin. Investigationof isolates to grow on inulin showed Pediococcus acidilactici isolate BE was able to consume inulin as the only carbon source. It is concluded that Pediococcus acidilactici isolate BE was a candidate probiotics and subject to further in vivo evaluation using animal models to examine their beneficial health effects.Key word : Pediococcus acidilactici, Lactobacillus casei, human origin and probiotics.

Studi Pakan Burung Perkici Pelangi (Trichoglossus haematodus Linnaeus, 1771) dalam Laboratorium Penangkaran W. Widodo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i3.2801

During the 2002-2003 period, the research was done to study 11 Rainbow Lorikeets reared in an animal house laboratory. The aim of this research was to find the food rations formule of the Rainbow Lorikeets so that those birds can be able to grow and breed well. The food rations were composed of 26.3% local bird foods (521), 35.09% lampung bananas, 8.77% slice corns, 10.5% boiled quails eggs, 1.75% white bread, bean sprouts and red sugar are 8.77%, respectively. All of food materials were mixed on the plastic cup and mixed with 450 ml of water, then pulverized like sweet porridge. That porridge was given to birds in cafeteria and the water was made ready “ad libitum” everyday. The results have shown that giving food rations formula can stimulate two pairs of the Rainbow Lorikeets breeding and during the 2002-2003 period they produced three young birds.

Perkembangbiakan Perkici Pelangi (Trichoglossus haematodus) di Laboratorium Penangkaran W. Widodo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.175

The research was conducted to understand the breeding aspects of the Rainbow Lorikeet (Trichoglossus haematodus), which were caged in flocks and as a pair, in two aviaries of the Animal House Laboratory of Zoological Division, the Research Center of Biology, Indonesian Institute of Sciences (LIPI), Cibinong. From January 2002 until January 2005, 11 birds of the Rainbow Lorikeets were used in this research,. The birds were divided into two groups, the first group (9 individuals) were caged in the first aviary and the second one (2 individuals) were caged in the second aviary. The size of each aviary room (LxWxH) was 3.32 m x 1.82 m x 2.85 m. The aviary rooms were equiped with the roosting, nest boxes, feeding and drinking water places. Foods and water were given in cafetaria everyday, starting from about 08.00 a.m. The foods consisted of bird food/”pur” (150 grams), white bread (15 grams), cooked quail eggs (60-70 grams), the lampung bananas (300 grams), slices of corn (100 grams), bean sprouts (50 grams), and palm sugar (50 grams). All of the igredients were mixed with 450-500 ml water and were then pulverized. The birds were also given some vegetables. The results were as follows: the Rainbow Lorikeets caged in flocks have produced two chicks and those in a pair only produced one chick. The clutchs consisted of 1-2 eggs. Eggs were white, smooth and their shape ovale. Average weight of the eggs was 7-8 grams. Weight of day old chicks was about 8 grams, those chicks were still blind and with out featheres (“altricial”). Incubation periods was 25-26 days, and incubated either by the female or the male. Fledging period was 71 days or more. The growing of the featheres and young birds of the Rainbow Lorikeets will be also discussed in this paper.

Co-Authors Asih Kurniawati Ety Aryati Nosa Septiana Anindita Tiyas Tono Taufiq Tutik Dwi Wahyuningsih Widya Asmara

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