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Study of Local Herb Potency as Rumen Modifier: Red Ginger (Zingiber Officinale Var. Rubrum) Addition Effect on In Vitro Ruminal Nutrient Digestibility Kurniawati, Asih; Yusiati, Lies Mira; Widodo, Widodo; Artama, Wayan Tunas
ANIMAL PRODUCTION Vol 21, No 1 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (430.093 KB) | DOI: 10.20884/1.jap.2019.21.1.713

Abstract

Red ginger is herb that commonly used as part of traditional medicine due to its essential oil content. Some essential oil have proven as rumen fermentation modifier. Addition of red ginger in ruminant diet was studied using in vitro gas production technique to evaluate its effect on nutrient digestibility. Red ginger meal was added to meet essential oil level in fermentation medium of 0 mg/l as control, 25, 50, 75 and 100 mg/l. The diet consisted of Penisetum hybride, rice bran, wheat pollard in ratio 60:20:20 DM basis. Feed fermentation was incubated for 24 h at 39°C. At the end of incubation data of gas production volume was taken, and residual feed were collected for further nutrient analysis to calculate the digestibility of dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF). Variance analysis was used to analysis collected data, followed by DMRT analysis.  Addition of red ginger increase total volume of gas production at level 50 mg/L and above. DM and OM digestibility did not affected by red ginger addition whereas CP digestibility were significantly decreased start at level 50 mg/l. In contrast, CF digestibility of treatment level of 50 mg/l significantly higher than control. In conclusion, addition of red ginger correspond to level essential oil of 50 to 100 mg/l improve ruminal nutrient fermentation.
TEKNIK PRODUKSI GAS IN-VITRO UNTUK EVALUASI PAKAN TERNAK : Volume Produksi Gas Dan Kecernaan Bahan Pakan Asih Kurniawati
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 3, No 1 (2007): Juni 2007
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.717 KB) | DOI: 10.17146/jair.2007.3.1.552

Abstract

In-vitro teknik produksi gas dapat digunakan untuk prediksi kualitas pakan. Pengaruh penambahan molase sebagai sumber karbohidrat mudah terdegradasi pada pakan sumber protein silase red clover dipelajari dengan menggunakan teknik ini. Dari data yang diperoleh menunjukkan terdapat korelasi positif antara total produksi gas dengan nilai kecernaan pakan (r = 0,96). Korelasi positif antara total produksi gas dengan produksi biomasa mikroba (r = 0,96). Penambahan molase sangat nyata meningkatkan nilai kecernaan bahan kering (P<0,01) meningkatkan bahan kering terdegradasi (P<0,01) dan produksi biomasa mikroba (P<0,01), serta meningkatkan efisiensi nitrogen pakan dalam pembentukan biomasa mikroba (P<0,01) seiring dengan peningkatan jumlah suplementasi. Penambahan 0,3 g molase menunjukan hasil yang tertinggi sementara penambahan 0,15 dan 0,225 molase memberikan hasil yang lebih bail dibanding penambahan 0,0625 molase dan red clover tanpa supelementasi. Dari hasil yang diperoleh dapat disimpulkan bahwa in-vitro teknik produksi gas dapat digunakan untuk evaluasi dan pemilihan bahan pakan untuk ternak.
Karakteristik fisis bioplastik yang dibuat dari kombinasi pati tapioka dan kasein susu apkir Ariya Dwi Nugrahanto; Asih Kurniawati; Yuny Erwanto
Majalah Kulit, Karet, dan Plastik Vol 37, No 2 (2021): Majalah Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20543/mkkp.v37i2.7422

Abstract

Penelitian ini bertujuan untuk mengembangkan bioplastik dari kombinasi pati tapioka dan kasein serta mengetahui karakteristik fisis, seperti ketebalan, densitas, transmisi cahaya, dan opasitas. Komposisi bioplastik dibuat dari dua jenis kasein, yaitu kasein komersial dan kasein susu afkir, dengan perbandingan pati tapioka dan kasein yang terdiri dari 4 kelompok perlakuan (4:0, 3:1, 2,5:1,5, 2:2). Setiap perlakuan diulang sebanyak 6 kali. Data karakteristik fsis, yang diperoleh, dianalisis menggunakan analisis varian rancangan acak lengkap two-way Anova. Jika terdapat perbedaan, dilanjutkan dengan uji Duncan multiple range test (DMRT). Hasil penelitian menunjukkan bahwa rasio pati dan kasein berpengaruh sangat nyata terhadap semua parameter uji (P<0,01). Peningkatan kasein komersial (KK) dan kasein susu apkir (KSA) meningkatkan ketebalan dan opasitas, sementara densitas dan transmisi cahaya menurun. Berdasarkan penelitian yang dilakukan, bioplastik dapat dibuat dengan kombinasi rasio pati tapioka dan jenis kasein yang berbeda dengan hasil terbaik pada rasio 2:2 yang menghasilkan ketebalan 0,29 mm, transmisi cahaya 20,03%, opasitas 2,51%, sementara pada densitas memiliki nilai terkecil 0,15 gr/cm3. Kesimpulannya bahan kasein dari susu apkir masih bisa digunakan sebagai biomaterial untuk pembuatan bioplastik dikombinasikan dengan tepung tapioka.
Human Origin Lactobacillus casei Isolated from Indonesian Infants Demonstrating Potential Characteristics as Probiotics in vitro W. Widodo; Tiyas Tono Taufiq; Ety Aryati; Asih Kurniawati; Widya Asmara
Indonesian Journal of Biotechnology Vol 17, No 1 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.461 KB) | DOI: 10.22146/ijbiotech.7852

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The aim of this experiment was to isolate and identify Lactic Acid Bacteria (LAB) from infant faecesand subsequent evaluation of its potential probiotics. LAB was isolated from faeces of infants who consumedbreast milk as the only source of diet on L-cysteine-supplemented MRS Agar, and incubated on 37oC for 48hours. Colonies grew on this media were then identifi ed based on morphological, physiological and molecularapproaches. Morphological and physiological identifi cations based on Gram staining, shape, motility, sporeformation, catalase, CO2 and NH3 production, and the ability to grow on temperature at 10oC and 45oC.Molecular identifi cation based on the amplifi cation of 16S rRNA gene. The potential application of selectedisolates for probiotics was evaluated based on the ability to grow on media with low pH and the additionof 0.5% bile salts, the ability to inhibit the growth of pathogenic Bacillus cereus and Eschericia coli, and in vitroadherence ability. On the basis of morphological, physiological and molecular analysis of 16S rRNA gene, itwas concluded that the selected isolate 1AF was a strain of Lactobacillus casei. Evaluation of probiotic in vitro showed that 60.4% of cells were resistant to pH 2.0 for 90 minutes. Survival of isolate 1AF after growing at0.5% bile salts was 70.8%. The selected isolate 1AF showed the ability to inhibit the growth of Eschericia coli and Bacillus cereus with inhibitory zone of 12.00±1,00 and 15.33±1.53 mm, respectively. In vitro study on theadherence value of isolate to solid plate was found at 46.5%. It is concluded that Lactobacillus casei isolate 1AFis a potential candidate as probiotics and subject to further in vivo evaluation.
PENGARUH PEMBATASAN PAKAN DAN PEMENUHAN KEMBALI PAKAN TERHADAP KONSUMSI NUTRIEN KECERNAAN NUTRIEN, STATUS FISIOLOGI DAN PERUBAHAN BERAT BADAN KAMBING BLIGON Bambang Suwignyo; Ulil Amri Wijaya; Rieska Indriani; Asih Kurniawati; Irkham Widiyono; Sarmin Sarmin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.11603

Abstract

Penelitian ini bertujuan untuk mengetahui kecernaan nutrien pakan dan status fisiologi pada kambing Bligon yang diberi perlakuan pembatasan pakan (feed restriction). Enam ekor kambing Bligon jantan umur rata-rata 12 bulan dengan bobot badan rata-rata 23,6 kg diberi pakan yang terdiri dari hijauanjerami kacang tanah (rendeng)dan konsentrat. Kambing dibagi menjadi 2 kelompok perlakuan. Tiga ekor kambing untukperlakuan kontrol diberi pakan berdasarkan kebutuhan bahan kering (BK) 3,5% dari bobot badan, dan tiga ekor kambing untuk perlakuan pembatasan pakandilakukan pembatasan pakan dengan pengurangan pakan 50% dari kebutuhan bedasarkan BK selama 35 hari. Variabel yang diamati adalah konsumsi bahan kering (BK), konsumsi bahan organik (BO), konsumsi total digestible nutrients (TDN), perubahan berat badan, kecernaan bahan kering (BK), kecernaan bahan organik (BO), temperatur tubuh, frekuensi respirasi, dan frekuensi detak jantung. Data yang diperoleh dianalisis dengan independent samplesT-Test. Hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa pembatasan pakan (feed restriction) berpengaruh nyata terhadap penurunan konsumsi bahan kering (BK), konsumsi bahan organik (BO), konsumsi total digestible nutrients (TDN), kecernaan bahan kering (BK) dan bahan organik (BO), tetapi tidak berpengaruh nyata pada temperatur tubuh, frekuensi respirasi, dan frekuensi detak jantung (pulsus) kambing Bligon. Pemenuhan kembali (refeeding) pakan berpengaruh nyata (P<0,05) menaikan kembali konsumsi BK dan konsumsi BO. Perubahan berat badan kambing mengikuti pola jumlah pemberian pakan. Saat pembatasan pakan, berat badan kambing perlakuan turun 55,24 g/hari dan ketika pakan dipenuhi kembali berat badannya meningkat 131,43 g/hari.
Konsumsi, Kecernaan Nutrien, Perubahan Berat Badan dan Status Fisiologis Kambing Bligon Jantan dengan Pembatasan Pakan Bambang Suwignyo; Ulil Amri Wijaya; Rieska Indriani; Asih Kurniawati; Irkham Widiyono; Sarmin Sarmin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6704.108 KB) | DOI: 10.22146/jsv.27560

Abstract

This study were aimed to determine the dry matter and organic matter intake, nutrients digestibility, body weight gain and physiological status of male Bligon goats treated with feed restriction. Six Bligon goats average age of 12 months and an average body weight of 23.6 kg were fed 40% of peanut raughages (rendeng) and 60% concentrate. Goats were divided into two (2) treatment groups. Control goat treatment were fed diets based on dry matter (DM) requirements 3.5% of body weight, and feed restriction goat treatment were fed diets with 50% reduction of DM requirement. Feed restriction was conducted for 35 days. The variables measured were dry matter (DM) and organic matter (OM) intake, total digestibility nutrient (TDN) intake, body weigh gain, dry matter (DM) and organic matter (OM) digestibility, rectal temperature, respiration frequency, and heart rate. Data were analyzed by independent samples T-test. The results showed that the feed restriction significantly decreasedconsumption of DM (676.07 ± 14.76 into 372.30 ± 53.08) and OM (639.38 ± 15.89 into 349.88 ± 49.35), consumption of TDN (461.60 ± 10.23 into 253.99 ± 36.17), DM digestibility (74.39 ± 1.60 into 71.27 ± 0.89) and OM digestibility (79.42 ± 1.54 into 76.34 ± 0.24), but had no significant effect on body  temperature, respiration frequency and heart rate (pulsus) of male Bligon goat. Value of ADG was 55.24 g/day (P0) then increased to 131.43 g/day when the feed was filled back. A feed restriction up to 50% decreased goat production performance, but did not interfere with the health status of livestock (can be indicated from physiological status of goat still in the normal range).
Effects of Four Essential Oils on Nutrients Digestibility of In Vitro Ruminal Fermentation Asih Kurniawati; Widodo Widodo; Wayan Tunas Artama; Lies Mira Yusiati
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i2.33522

Abstract

This research was done to study the effect of four essential oils (EOs), as feed additives, on ruminal nutrient digestibility in order to find out candidate of rumen modifier. Those four EOs were destilated from Foeniculum vulgare (Mill), Pinus merkusii (Jungh. & de Vriese), Cymbopogon nardus (L.) Rendle and Melaleuca leucadendra (L.). Their effect on dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF) digestibility were studied using in vitro rumen fermentation technique according to Theodorou method. Data were statistically analysed using analysis of variance factorial 4x5 design. Feed for fermentation substrate consist of Pennisetum purpureum, rice bran and wheat pollard. Essential oil individually was added and mix with substrate to meet the final concentration in fermentation media of 0, 100, 200, 400 and 800 mg/l. P. merkusii, C. nardus and M. leucadendra EOs reduced DM, OM, and CF digestibilities. The decreasing were in line with the increasing of EOs doses. Therefore F. vulgare only reduced CF digestibility. The value of DM, OM and CP digestibilities in fermentation with F. vulgare did not differ from control. CP digestibility differed among EO treatments. In F. vulgare, and P. merkusii, treatments, CP digestibility did not differ from control, whereas overall CP digestibility in C. nardus tended to be higher than control and CP digestibility in M. leucadendra was significantly higher. All EOs addition reduced CF digestibility at all level. CF digestibility in fermentation added 800 mg/l of M. leucadendra EO was slumped to only 14.29% of control. Among four EOs M. leucadendra is the most potent on interrupt rumen feed fermentation whereas P. merkusii and C. nardus in moderate ways and F. vulgare in delicate manner.
ADDITION OF ESSENTIAL OIL SOURCE, Amomum compactum Soland ex Maton AND ITS EFFECT ON RUMINAL FEED FERMENTATION IN-VITRO Asih Kurniawati; Widodo Widodo; Wayan Tunas Artama; Lies Mira Yusiati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 3 (2019)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.771 KB) | DOI: 10.11598/btb.2019.26.3.1089

Abstract

Essential Oil (EO), as feed additive, is known to increase the feed efficiency and reduce the methane production among ruminants. This research was done to study the effect of java cardamom (Amomum compactum Soland ex Maton) essential oil as feed additive on ruminal feed fermentation. The in vitro gas production technique was used in this research to determine the effect of cardamom on nutrient fermentation in the rumen. Cardamom meal was added into the feed sample to get end concentration of EO in the fermentation medium as much as 0, 25, 50, 75 and 100 mg/l. The substrate consisted of Pennisetum purpureum, rice bran and wheat pollard. The addition of cardamom did not significantly affect the digestibilityof dry matter   except at 100 mg/l in which it decreased. Protein digestibility decreased when the diet was added with cardamom, whereas organic matter and crude fiber digestibility increased up to 13.5% and 24% level of EO100mg/l respectively.  The production of volatile fatty acid (acetate, propionate, butyrate), pH and microbial protein synthesis except the ammonia concentration, were not affected by cardamom addition. Similarly, the methane production and protozoa population did not significantly change. The utilization of java cardamom as feed additive positively affected the ruminal feed fermentation by increasing the organic matter and crude fiber digestibility and reducing the protein digestibility.
EVALUASI PERFORMA PRODUKSI LEGUM KEMBANG TELANG (Clitoria ternatea) MENGGUNAKAN PUPUK FOSFOR DAN MOLIBDENUM: EVALUATION OF LEGUM PRODUCTION PERFORMANCE OF Clitoria ternatea (Clitoria ternatea) USING PHOSPHORUS AND MOLYBDENUM FERTILIZERS Prihutomo; Umami, Nafiatul; Kurniawati, Asih; Sulistijo, Edi Djoko; Gusri, Rahmat; Yoga Kertiyasa, I Kadek
Journal of Animal Research and Applied Science Vol. 5 No. 2 (2024): Desember 2024
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/aras.v5i2.38462

Abstract

Kembang telang (Clitoria ternatea) adalah tanaman leguminosa unggulan yang tersebar luas di wilayah tropis dengan kandungan nutrisi tinggi sehingga sangat baik untuk mendukung produksi ternak ruminansia. Fosfor dan molibdenum adalah unsur hara esensial yang berkontribusi dalam meningkatkan kualitas tanaman ini. Penelitian ini bertujuan untuk menganalisis dampak pemberian fosfor dan molibdenum dalam berbagai variasi dosis terhadap produksi berat kering dan karakteristik tumbuh Clitoria ternatea. Penelitian dilakukan menggunakan rancangan acak lengkap pola split-plot dengan 9 perlakuan dan 3 ulangan, terdiri dari BC1D0, BC1D1, BC1D2, BC2D0, BC2D1, BC2D2, BC3D0, BC3D1, BC3D2 dengan variabel yang diamati yaitu Berat Kering Daun, Berat Kering Batang, Berat Kering Akar, Berat Kering Total Hijauan, Nisbah Berat Kering Daun dengan Berat Kering Batang, serta Nisbah Berat Kering Total Hijauan dengan Berat Kering Akar. Hasil penelitian menunjukkan bahwa pemberian pupuk fosfor 0,44 g/polibag dan molibdenum 0,00789 g/polibag (D2) memberikan pengaruh signifikan (P<0,05) terhadap Berat Kering Daun sebesar 33,74 gram, Berat Kering Batang 42,65 gram, Berat Kering Akar 17,54 gram, Berat Kering Total Hijauan 76,39 gram, Nisbah Berat Kering Daun dengan Berat Kering Batang sebanyak 1,63 %, serta Nisbah Berat Kering Total Hijauan dengan Berat Kering Akar sebanyak 0,23 % pada tanaman leguminosa Clitoria ternatea. Kesimpulan dari penelitian ini bahwa penggunaan dosis pupuk fosfor sebanyak 0,44 g/polibag dan molibdenum 0,00789 g/polibag (D2) dapat meningkatkan produksi berat kering dan karakteristik tumbuh pada legum Clitoria ternatea. Selain itu, penanaman dengan menggunakan dua tanaman legum Clitoria ternatea per polibag (BC2) dapat menghasilkan produksi berat kering tertinggi, dikarenakan rendahnya persaingan antar tanaman dalam memanfaatkan unsur hara.
Sido Makmur Srandakan Bantul Livestock Farmer's Group Readiness to Utilize Fermented Feed to Increase Income Kurniawati, Asih; Muhlisin, Muhlisin; Kusumastuti, Tri Anggraeni
Jurnal Sain Peternakan Indonesia Vol 20 No 3 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.3.135-141

Abstract

The research aims to analyze the group's understanding and readiness in utilizing fermented feed preservation and income analysis, specifically focusing on the selection of a location within the Sido Makmur group in Srandakan District, Bantul Regency. The sample was selected as a convenience sample of as many as 17 out of 80 members who were willing to be interviewed. The research was conducted in a quantitative descriptive manner. Primary data was collected through direct interviews using a questionnaire tool. The primary data collected include respondent characteristics, land ownership, and readiness to utilize feed preservation. The results show that most farmers of productive age with formal education equivalent to high school have received training in feed preservation; their main occupation is farming. The forage potential comes from agricultural waste, plantations, grass, and legumes. Farmers are willing to provide fermented feed to livestock (82.50%) and interested in selling fermented feed (76.47%) in the form of drums with a capacity of 50 kg made by farmer’s group (47.06%), created by individual (70.59%) and plastic packaging (41.18%) Income from fermented feed in drum was IDR 37,970 and plastic was IDR 35,970. Selling price and product quality are essential factors in selling fermented feed. Fortunately, to support product quality, packaging and labelling support is needed.