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All Journal JURNAL KIMIA SAINS DAN APLIKASI Acta Pharmaceutica Indonesia
Muhamad Insanu
Kelompok Keilmuan Biologi Farmasi, Sekolah Farmasi, ITB Bandung
Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

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ISOLASI DAN IDENTIFIKASI GLIKOSIDA FLAVONOID BIOAKTIF DARI SYZYGIUM JAMBOS DAN AKTIVITAS PENGHAMBAT ENZIM α-GLUKOSIDASE Meilani, Lani; Nor, Islan; Insanu, Muhamad
Acta Pharmaceutica Indonesia Vol. 49 No. 2 (2024)
Publisher : School of Pharmacy Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes has grown into a demanding global public health problem, with generally 537 million individuals afflicted worldwide. Without appropriate intervention, the risk of developing chronic complications increases. One of the practical actions for controlling type 2 diabetes mellitus is suppressing carbohydrate-hydrolyzing enzymes such as α-glucosidase. Syzygium jambos has a long history as a traditional medicine. It's commonly used to treat diabetes, rheumatism, inflammatory pain, and as a diuretic. This study aimed to investigate the α-glucosidase inhibitory properties of S. jambos leaves and their isolated compounds. Ethanol was used as the solvent for maceration, followed by fractionation using liquid-liquid extraction, vacuum liquid chromatography, column chromatography, and radial chromatography. The isolated compounds were identified using thin-layer chromatography (TLC), TLC densitometry, shifting reagents, infrared (IR) spectroscopy, mass spectrometry, and nuclear magnetic resonance (NMR) spectrometry. The ethyl acetate fraction presented the most potent activity of α-glucosidase inhibitory, with an IC50 value of 0.31 ± 0.01 µg/mL, exceeding the acarbose as positive control (IC50 62.86 ± 1.24 µg/mL). Two flavonoid glycosides, quercetin 3-O-xylosyl-(1→2)-rhamnoside and myricetin 3-O-xylosyl-(1→2)-rhamnoside, were identified inside this fraction, with IC50 values of 65.63 ± 1.36 µg/mL and 188.96 ± 2.85 µg/mL, respectively. These findings suggest that S. jambos has potential as a natural source for developing dietary supplements to control hyperglycemia affiliated with type 2 diabetes mellitus.
Potential Antioxidant Activity of the Unused Part of Tamarillo (Solanum betaceum Cav.) Choirunnisa, Atina Rizkiya; Regiana, Zhafira Amalia; Insanu, Muhamad; Hartati, Rika; Pramastya, Hegar; Rizaldy, Defri; Fidrianny, Irda
Jurnal Kimia Sains dan Aplikasi Vol 28, No 6 (2025): Volume 28 Issue 6 Year 2025
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.28.6.336-344

Abstract

Tamarillo (Solanum betaceum Cav.) is a member of the Solanaceae family that contains phenolic and flavonoid compounds. The fruit of the tamarillo is commonly consumed. However, other parts that are rarely used also have many potential benefits, including antioxidant activity. This study aims to evaluate antioxidant activity of used part (flesh) and unused part (leaves and peel) extracts of tamarillo through ascorbic acid equivalent antioxidant capacity (AEAC), phenolic and flavonoids content, correlation the phenolic and flavonoid content on antioxidant activity, correlation the DPPH and CUPRAC methods and the levels of flavonoid compound in selected extract. Three organs (flesh, leaves, peel) were extracted using polarity solvents (n-hexane, ethyl acetate, ethanol). Antioxidant activity was conducted using DPPH and CUPRAC assays, and total phenolic acid and flavonoid acid were also investigated. The result showed that the highest antioxidant activity of tamarillo extracts using DPPH and CUPRAC methods was given by ethanol leaves, 94.917 ± 3.920 and 110.182 ± 7.987 mg AEAC/g sample. The highest TPC in the ethanol leaves extract 10.416 g GAE/100 g, while the highest TFC in the n-hexane leaves extract 8.367 g QE/100 g. Phenolic and flavonoid compounds were identified as significant contributors to the antioxidant activity, with both assessment methods showing a strong linear correlation. In conclusion, Tamarillo leaves had the potential to be a natural antioxidant source, with a rutin content of 0.088%, according to HPLC analysis.
Biological activities study of Cinnamon (Cinnamomum burmanni (Nees & T.Nees) Blume) as Cosmetic Ingredients Rizaldy, Defri; Insanu, Muhamad; Hartati, Rika; Fadhilah, Ismah Ibtihal; Putri, Rian Destiyani; Cheong, Min Kyoung
Acta Pharmaceutica Indonesia Vol. 48 No. 2
Publisher : School of Pharmacy Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/api.v48i2.22472

Abstract

Objectives: The goals of this research were to study the biological activities of cinnamon bark extract related to its use as cosmetic ingredients. The potential use of cinnamon bark as cosmetic preparations was studied by confirming its antioxidative, antimicrobial, and its inhibition towards tyrosinase activity. Methods: Extraction was performed by maceration using 70% ethanol. Antioxidative activity using the DPPH methods, melanin inhibitory effect was determined using in vitro anti tyrosinase assay. MHC and MIC of cinnamon bark extract were determined towards Staphylococcus aureus, Staphylococcus epidermidis, and Cutibacterium acnes. Results: The standardization result of the crude drugs showed positive result on qualitative analysis of Flavonoid, Tannin, and Quinone groups. Flavonoid content also found on the ethanolic extract. The IC­50 antioxidative activity of cinnamon ethanolic was 7.52 ± 0.13 μg/mL compared to ascorbic acid with 2.54 + 0.02 μg/mL. The Ethanolic extract of cinnamon inhibited tyrosinase with IC­50 of 9.44 ± 0.26 mg/mL. Lowest minimum inhibitory concentration of ethanolic extract was found towards Cutibacterium acne with 1000 μg/mL. Whereas weak antimicrobial activity was showed towards Staphylococcus aureus and Staphylococcus epidermidis with MIC and MBC value started from 2000 μg/mL. Conclusion: Comparable result on antioxidative and anti-tyrosinase analysis shows that cinnamon bark have fair potential to further developed and formulated for topical maintenance product.
Co-Authors Cheong, Min Kyoung Choirunnisa, Atina Rizkiya Fadhilah, Ismah Ibtihal Irda Fidrianny Meilani, Lani Nor, Islan Pramastya, Hegar Putri, Rian Destiyani Regiana, Zhafira Amalia Rika Hartati Rizaldy, Defri