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25. The effect of Fingerroot Rhizome (Boesenbergia pandurata) Extract on the Growth of Staphylococcus aureus in Vitro Silvia P.N Keliat; Darniati Darniati; Abdul Harris; Erina Erina; Rinidar Rinidar; Fahkrurrazi Fahkrurrazi
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.3654

Abstract

This study aimed to determine the inhibitory effect and the concentration of fingerroot extract to inhibit Staphylococcus aureus growth. The data was analyzed descriptively. Ampicillin was used as positive control, distilled water was used as negative control, and the treatments were given fingerroot extract with a concentration of 5%, 15%, 25%, 35% and 45%. This study was conducted with three replications. The parameter measured was the diameter of inhibition zone formed by diffusion method. The average diameter of inhibition zone of the fingerroot extract were 15% : 10.3 mm; 25% : 13,6mm; 35% : 18,7mm; 45% ± 21,1mm, and at a concentration of 5% the inhibition zone is not formed. The final conclusion is that the fingerroot extract can inhibit the growth of Staphylococcus aureus. The higher concentration of fingerroot extract, the more extensive inhibition zone formed.
23. Effect of Temu Kunci’s Root (Boesenbergia pandurata) Extract to Pseudomonas aeruginosa Florentina Magdalena Girsang; T. Armansyah; Mahdi Abrar; Erina Erina; Darniati Darniati; Nuzul Asmilia
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.3649

Abstract

This study aimed to determine the effect of temu kunci’s extract (Boesenbergia pandurata) on the growth of Pseudomonas aeruginosa in vitro. This research was conducted using seven treatments with three replications. The experiment was carried out using Kirby Bauer’s method, the number of bacteria used was in accordance with the standards of Mc Farland 1. Blank discs were soaked in temu kunci’s extract in concentration of 10, 20, 30, 40, and 50%. Streptomycin was used as a positive control and sterile aquades as a negative control. All the discs were placed on the surface of bacteria cultured on MHA and incubated for 24 hours at 37 °C. Diameter of inhibition zone formed was measured by using a caliper. Data were analyzed descriptively. The average diameter of inhibition zone of temu kunci’s extract with a concentration of 10, 20, 30, 40 and 50% were 8.00, 12.30, 13.85, 19.16, and 19.86 mm, respectively. The average diameter of inhibition zone formed on the positive control and negative control were 14,25 and 0 mm, respectively. The conclusion showed that the temu kunci’s extract (Boesenbergia pandurata) has the ability to inhibit the growth of Pseudomonas aeruginosa.