Erina Erina
Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

ISOLASI Escherichia coli PADA TELUR PUYUH (Coturnix coturnix japonica) YANG GAGAL MENETAS DI PETERNAKAN DESA GAROT KECAMATAN DARUL IMARAH ACEH BESAR (Isolation of Escherichia coli from Failed to Hatch Quail’s Eggs (Coturnic coturnix japonica) in Garot, Darul Imarah Subdistrric, Aceh Besar) Chairul Saputra Siregar; Erina Erina; Mahdi Abrar
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.29 KB) | DOI: 10.21157/jim vet..v2i2.6658

Abstract

ABSTRAK                Penelitian ini bertujuan mengisolasi bakteri Escherichia coli pada telur puyuh yang gagal menetas di Desa Garot Kecamatan Darul Imarah, Kabupaten Aceh Besar. Sebanyak 30 butir telur puyuh yang gagal menetas diperiksa di Laboratorium Mikrobiologi Fakultas Kedokteran Hewan, Universitas Syiah Kuala Banda Aceh. Sampel diisolasi berdasarkan metode Carter yang dimodifikasikan. Telur dibuka, diambil embrionya dan diswab dengan swab steril. Selanjutnya dimasukkan dalam media Nutrien Broth (NB) dan inkubasi pada suhu 37ºC selama 24 jam. Kemudian dengan menggunakan ose steril, biakan dipindahkan ke media Eosin Methilen Blue Agar (EMBA). Bakteri yang tumbuh diamati morfologi koloninya dan dilakukan pewarnaan Gram. Hasil penelitian menunjukkan bahwa 3 dari 30 sampel (10%) telur burung puyuh yang diteliti positif terinfeksi Escherichia coli . Oleh sebab itu dapat disimpulkan bahwa Escherichia coli merupakan salah satu penyebab kegagalan menetas pada telur puyuh dipeternakan Desa Garot Kecamatan Darul Imarah, Kabupaten Aceh BesarABSTRACTThis study aimed to isolate Escherichia coli from quail eggs that were not hatched in a farm at Garot village, Darul Imarah subdistrict, Aceh Besar District. Thirty unhatched quail eggs were examined in microbiology laboratorium of Veterinary Faculty of Syiah Kuala University, Banda Aceh. The eggs opened and the embrio were swabbed, and put into Nutrien Broth (NB) and incubated at 37o C for 24 hours. The culture were then transfered onto Eosine Methilen Blue Agar (EMBA). Grown bacteria were observed for it’s colony  morphology and stained with Gram stain. The result showed that 3 out of 30 unhatched samples (10%) were positively infected by Escherichia coli. Based on this study, it can be concluded that Escherichia coli was one of many factors that caused quail eggs failed to hatch in a farm of  Garot village, Darul Imarah subdistrict, Aceh Besar District.
Deteksi Cemaran Bakteri Shigella Sp. Pada Ikan Kuniran (Upeneus Sulphureus) Di Pasar Al-Mahira Banda Aceh Syarifah Alifya; Erina Erina; Andi Novita; Rastina Rastina; M Daud AK; Hennivanda Hennivanda
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 4 (2022): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i4.21507

Abstract

ABSTRAKIkan yang dijual di pasar tidak menutup kemungkinan terkontaminasi oleh bakteri. Penelitian ini bertujuan untuk mendeteksi adanya cemaran bakteri Shigella sp. pada ikan kuniran (Upeneus sulphureus) di pasar Al-Mahira Banda Aceh. Penelitian ini menggunakan metode survei cross-sectional dan pengambilan sampel menggunakan metode sampling jenuh (sensus). Pemeriksaan sampel menggunakan metode Carter. Sampel yang digunakan pada penelitian ini sebanyak 12 ekor ikan, yang diambil dari 4 pedagang ikan di pasar Al-Mahira Banda Aceh. Ikan dibedah secara aseptis dan ususnya diambil kemudian dimasukkan ke dalam media Nutrient Broth (NB) dan diinkubasi pada suhu 37℃ selama 24 jam. Kemudian dilakukan penanaman pada media Salmonella Shigella Agar (SSA) dan diinkubasikan kembali pada suhu 37℃ selama 24 jam. Hasil penanaman pada media SSA menunjukkan bahwa semua sampel positif terkontaminasi Shigella sp. yang ditandai dengan adanya bulat berwarna bening pada koloni yang ditanam. Selanjutnya koloni bakteri yang tumbuh dilakukan pewarnaan Gram dan diperiksa secara mikroskopis. Hasil pewarnaan Gram menunjukkan bakteri yang terlihat berwarna pink/merah dan berbatang pendek, yang menunjukkan bakteri tersebut adalah genus Shigella sp. Dari hasil penelitian dapat disimpulkan bahwa semua sampel yang diperiksa positif tercemar bakteri Shigella sp.ABSTRACTFish sold in the market does not rule out being contaminated by bacteria. This study aims to detect the presence of bacterial contamination Shigella sp. on kuniran fish (Upeneus sulphureus) at Al-Mahira market Banda Aceh. This study used a cross-sectional survey method and sampling using the sampling jenuh method. Samples were examinated using the Carter method. The samples used in study were 12 fish taken from 4 fish traders in the Al-Mahira market Banda Aceh. Fish were dissected aseptically and their intestines were removed and the put in Nutrient Broth (NB) media and incubated at 37 ℃ for 24 hours. Then it was planted on Salmonella Shigella Agar (SSA) media and re-incubated at 37 ℃ for 24 hours. The results of planting on SSA media showed that all positive samples were contaminated with Shigella sp. which was indicated by presence of clear colored circles on the planted column. Futhermore, the column of bacteria that grew was stained with Gram stain and examined microscopically. The result of Gram staining showed that the bacteria were pink/red and had short trunks, which indicated that the bacteria were of genus Shigella sp. The result of the study can be concluded that all samples used were positively contaminated with Shigella sp.
12. Isolate Of Bacteriua Cellulase Enzyme Production In The Rumen Of Aceh Cattle Base On Analysis Homology 16S rRNA Indah Melzana; Safika Safika; Darmawi Darmawi; Erina Erina; Fakhrurrazi Fakhrurrazi; Al Azhar
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.3415

Abstract

The rumen of cattle is well known one of a bacterium cellulolitic production that have ability to degrade cellulose for produce cellulase enzymes. The aims of the present study is to determine the type of bacteria produced cellulase enzymes in the rumen of cattle aceh. The diversity of cellulolitic bacteria is determined by  analysis of 16S rRNA genes. Isolation of cellulolytic bacteria, total DNA isolation, agarose gel electrophoresis, amplification of the 16S rRNA gene, determining the DNA sequence, DNA sequence homology and alignment of DNA sequences. Isolation of bacterium cellulolitic, isolating total DNA, gel electroforesis agarosa, amplified of gen 16S rRNA, determination the DNA barcode,  homology DNA barcode, and alignment of DNA sequences. The sample of rumen liquid was taked from RPH , then maked isolation of total DNA to get pure DNA from bacterium whos result and amplification DNA of bacterium cellulolitic separated by electroforesis gel agarosa. Gene of bacterium cellulolitic was isolated based Polymerase Chain Reaction methode. Alignment is done by using BlastN program, then use CLustalW program.  It can be concluded that the species of bacteria (FKH_USK_6) in rumen has a closeness with Coronabacter sakazakii bacteria strain PNP8 of 97% according to the sizeof the DNA sequence and the same position on an existing gene in GenBank.
Aspergillosis in the Quail (Coturnix japonica) Natasha Natasha; Erina Erina; M. Hasan
Jurnal Medika Veterinaria Vol 16, No 1 (2022): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v16i1.9949

Abstract

This study aimed to isolate and identify fungus that causes aspergillosis in the quail (Coturnix japonica). The samples used in this study were 30 pairs quail lungs taken randomly from the Hanin Group Quail Farm, Garot village, Darul Imarah subdistrict, Aceh Besar regency. The growth of the fungus was observed for Aspergillus sp. macroscopically and microscopically. The data were analyzed descriptively. The results of this study revealed that from 30 pairs quail lungs, Aspergillus sp. can be isolated from 26 out of 30 lungs. It can be concluded that 86.67% of the lung samples were infected with Aspergillus sp.
Obervation of Thymus Regression Time of ALPU chicken Kamiliyatul Fadhilah; Erina Erina; M Aman Yaman
Jurnal Medika Veterinaria Vol 16, No 2 (2022): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v16i2.10221

Abstract

This study aimed to determine the number of lobes and thymus regression time of ALPU chickens. The samples were 76 ALPU chickens aged 1 – 19 weeks, consisted of 38 male and 38 female chickens. Two male and 2 female chickens were sacrificed every week and the thymus were collected and weighed. The results showed that the increase in thymus weight occurred in the age range of 1 – 15 weeks and reached the maximum weight at the age of 15 weeks. Thymus was decreased in weight at the age of 16 – 17 weeks. Thymus regressesion occurred at the age of 18-19 weeks. The thymus had 5-9 lobes. The result of T test on the average weight of ALPU chicken thymus showed that the weight of male ALPU chicken thymus of starter phase compared with grower phase was significantly different (p0.05), while the grower phase with finisher phase was not significant or there was no difference (p0,05). The result of T-test for weight of female ALPU thymus showed that the weight of thymus of starter phase compared to grower phase chickens was significantly different (p0.05), whereas grower phase with finisher result was not significantly difference (p0.05).Therefore, it was concluded that the number of thymus lobes of ALPU chickens are 5 - 9 lobes and the time of thymus regression of ALPU chicken occurs at the age of 18 - 19 weeks.
The Regression Time of Bursa of Fabricius in ALPU Chickens Riri Agusmanepi; Erina Erina; M Aman Yaman
Jurnal Medika Veterinaria Vol 16, No 1 (2022): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v16i1.10138

Abstract

This study aimed to determine the regression time of bursa Fabricius in ALPU chicken. In this research, 72 chickens of ALPU consisted of 36 males and 36 females, aged 1 week, 2 weeks,, until 18 weeks old were used. Each age group consisted of 2 male chickens and 2 female chickens. Bursa Fabricius of every age group was collected and weighed. The results showed that the weight of bursa Fabricius increased at 1 to 15 weeks old, and reached the maximum weight at 15 weeks of age. After reaching maximum weight, bursa Fabricius underwent regression that occurred at 16 to 18 weeks old. Bursa Fabricius’s weight increased in the starter phase at 1 to 6 weeks, grower at 7 to 12 weeks and will regress in the finisher phase at 13 to 18 weeks. The results of the average weight analysis of bursa Fabricius using t-test was observed by comparing the weight of bursa Fabricius ALPU chicken starter phase with grower phase and grower phase with finisher phase. The weight of bursa Fabricius of male ALPU on starter phase with grower phases was significanly difference (p0.05) whereas grower phase with finisher phase was not significantly difference (p 0,05). The weight of bursa Fabricius of female ALPU on starter phase with grower phases was significanly difference (p0.05), whereas grower phase with finisher phase was not significant (p 0,05). Therefore, it can be concluded that regression of bursa Fabricius occurred at the age of 16 to 18 weeks. The weight of bursa Fabricius in starter phase and grower phases are significantly difference, however there was no significantly difference was observed in finisher phase.