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All Journal Biota BIO-CONS: Jurnal Biologi dan Konservasi
Dian Catur Prayantini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Energy Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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Pollen Viability and Microspore Culture in Three Broccoli Cultivars (Brassica oleracea L. var. italica Plenck) Septarini Dian Anitasari; Ida Ayu Astarini; Made Ria Defiani; Made Pharmawati .; Dian Catur Prayantini
Biota Vol 5 No 2 (2019): Jurnal Biota 2019
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v5i2.3340

Abstract

Broccoli is a high value vegetable crop in Indonesia, however production is low due to limited number of suitable cultivars, so, breeding hybrid broccoli for warm climate is important. The first step in hybridization is providing homozygote parent plants which can be done efficiently via microspore culture. The objectives of this study were to determine 1) bud size that produce uninucleate microspore stage appropriate for culture; 2) pollen viability, 3) microspore development, in three broccoli cultivars (‘BL 10001’, ‘Royal Green’ and ‘Green Magic’). Various bud size (1 – 5 cm) was squashed and observed microscopically to determine bud size containing uninucleate microspore. Pollen viability was determined by IKI staining and pollen germination method. Chromosome number was counted on root tips using squash method with aceto-orcein stain. Various heat treatment schemes were conducted to induce microspreo development. Result showed uninucleate microspore derived from 2-3 mm and 3-4 mm bud length of ‘BL-10001’ and ‘Royal Green’ was responsive for microspore development in culture. Pollen viability varied among cultivars, 78-87% on IKI method and 15-16% on germination test. Microspore culture showed different embryogenesis response; pollen-like structure was produced by ‘BL 10001’.
Penentuan Jumlah Kromosom pada Tiga Kultivar Brokoli BL 10001, Royal Green, dan Green Magic Septarini Dian Anitasari; Ida Ayu Astarini; Made Pharmawati; Made Ria Defiani; Dian Catur Prayantini; Dwi Nur Rikhma Sari
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 2 No. 1 (2020): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v2i1.326

Abstract

Brokoli (Brassica oleracea L. var. italica Plenck) merupakan sayuran yang memiliki nilai gizi tinggi dan popular di Indonesia. Produksi brokoli di Indonesia rendah karena umumnya kultivar yang ditanam di Indonesia adalah kultivar introduksi. Kultivar introduksi tidak cocok di lingkungan tropis Indonesia sehingga memerlukan upaya pemulian tanaman untuk perbaikan kultivar. Salah satu upaya perbaikan kultivar yang popular saat ini adalah kultur mikrospora. Kultur mikrospora dapat memproduksi tanaman homozigot atau galur murni sehingga nantinya dapat dijadikan tetua dalam program pemuliaan tanaman. Penentuan Jumlah kromosom sangat berguna dalam pengembangan kultur mikrospora dan pemuliaan tanaman hibrida. Kultur mikrospora menghasilkan variasi jumlah kromosom yang beragam sehingga pengetahuan jumlah kromosom penting dilakukan dalam kultur mikrospora. Penelitian ini menggunakan 3 kultivar brokoli Green Magic, Royal Green dan BL 10001. Penentuan jumlah kromosom menggunakan metode Squash dengan pewarna aceto-orcein pada ujung akar. Hasil penelitian menunjukkan jumlah kromosom pada kultivar BL 10001 adalah 18 kromosom, pada kultivar Royal Green adalah 9 kromosom dan pada kultivar Green Magic adalah 14 kromosom.
Co-Authors Dwi Nur Rikhma Sari IDA AYU ASTARINI MADE PHARMAWATI Made Ria Defiani Septarini Dian Anitasari Septarini Dian Anitasari