Lily Ismaini
Cibodas Botanical Garden-Indonesian Institute of Sciences

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ANALISIS SENYAWA BIO AKTIF DARI EKSTRAK BUI PICUNG (Pangium edule Reinw.) SEGAR Mangunwardoyo, Wibowo; Ismaini, Lily; Heruwati, Endang Sri
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.188 KB) | DOI: 10.14203/beritabiologi.v9i3.781

Abstract

Pangi (Pangium edule Reinw.) seed has long been used traditionally as a preservative agent for fish, especially in remote areas.A study has been conducted on analysis of bioactive compounds of pangi fresh seeds extracts and their fractions.In this study,maceration of fresh seed using water and 50% ethanol was carried out followed by thin layer chromatography (TLC) analysis to see weather the extracts contained tannin. Both extract then separated into their respective fractions using column chromatography.Fractions which had been tested to have highest antibacterial activity were then analysed using gas chromatography mass spectrometry (GC-MS) to assess the active compounds which believe to be a preservative agent.Identification of water and 50% ethanol extract of Pangium edule Reinw.fresh seeds with TLC resulted that tannin were found in those extracts. Furthermore, GC-MS analysis showed that fractions which had been previously tested to have high antibacterial activity contained 9-octadecanoic acid whith similarity index of 89%, and 1,2-benzenedicarboxylic acid whith similarity index of 94-85%.
An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus sp.) in Cibodas Botanical Garden Surya, Muhammad Imam; Ismaini, Lily; Destri, Destri; Normasiwi, Suluh
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6559

Abstract

Currently, Cibodas Botanical Garden (CBG) has a domestication programme of wild raspberries (Rubus sp.) from Indonesia mountain forest. One of the activities in the domestication programme is fruit breeding. In order to improve the quality of wild raspberry, two mutagens i.e. chemical (oryzalin) and physical (gamma rays) mutagens were used in the breeding programme. Moreover, seed of wild raspberries from the collection of CBG were used in the programme of mutation breeding. The results showed that each species of wild raspberries has different response on the mutagen. Percentage of seed germination were affected by mutagen of oryzalin and gamma rays. Rubus fraxinifolius and Rubus rosifolius seeds were still able to germinate after treated by 100 M of oryzalin. In the other hand, with increase in radiation above 300 Gy a reduction in percentage of germination was observed in M1 generation of Rubus lineatus and Rubus chrysophyllus.How to CiteSurya, M. I., Ismaini, L., Destri, D. & Normasiwi, S. (2016). An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus spp.) in Cibodas Botanical Garden. Biosaintifika: Journal of Biology & Biology Education, 8(3), 331-335.
Study on In Vitro Growth of Rubus fraxinifolius Mutant (m1) Resulted from Gamma-Ray Irradiation (60Co) Ismaini, Lily; Normasiwi, Suluh; Surya, Muhammad Imam; Destri, Destri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 6, No 2 (2018)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2018.082.70-76

Abstract

Rubus fraxinifolius belonging to the wild raspberry group has not been developed in Indonesia. Mutation breeding using gamma-ray as mutagen which was combined with in vitro culture is one of acceleration effort to obtain superior characteristics of the fruit crops, such as larger fruit size, higher nutrition content, plant with less of spines and fruit storage ability. The R. fraxinifolius seeds were irradiated with different doses of gamma-ray ranging from 0 to 500 Gy using the 60Co (Cobalt). This research aimed to determine in vitro growth of R. fraxinifolius mutant (M1) after irradiation using gamma-ray. The results showed that the highest percentage of seed germination was obtained on the doses of 100 and 200 Gy. Furthermore, the subculture of R. fraxinifolius mutant (M1) on MS medium with the addition of BA showed the different growth on number of shoots, number of leaves, and plantlet height. Moreover, R. fraxinifolius control showed higher value of shoots, number of leaves, and plantlet height at 4 and 8 weeks after subculture compared to R. fraxinifolius mutant. MS medium with the addition of IBA showed that the number of roots of R. fraxinifolius control (5.75) was higher than that of R. fraxinifolius mutant (M1) (4.83).
Fruit Nutrients of Five Species of Wild Raspberries (Rubus spp.) from Indonesian Mountain’s Forests Surya, Muhammad Imam; Suhartati, Siti; Ismaini, Lily; Lusini, Yuyun; Destri, Destri; Anggraeni, Dian; Normasiwi, Suluh; Asni, Nurul; Sidiq, Mirwan Abu Bakar
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (709.404 KB) | DOI: 10.11594/jtls.08.01.13

Abstract

Rubus spp. (wild raspberries) is one of genera belongs to Rosaceae, also distributed in the mountain forest of Indonesia. It has a high potency to be cultivated plant. Although, some of the potential of Rubus has already known, information of about fruit nutrients of species that distribute in the mountain forest of Indonesia are not known yet. This study was aimed to determine the fruit nutrients of five species of wild raspberries that was collected in Cibodas Botanical Garden. The results of our experiments demonstrate that the fruits nutrients contents varied among five species of wild raspberries from Indonesian mountain's forests. Rubus fraxinifolius has the highest content of sugar (5.05 g sugar per 100 g fruits) compared to Rubus rosifolius, Rubus chrysophyllus, Rubus lineatus and raspberry. During the ripening and ripe period, there were a different value of vitamin C and iron contents. The highest vitamin C of R. fraxinifolius (83.65 mg/100 gram) on the ripening fruit stage II. On the other hand, the highest vitamin C of R. rosifolius (54.30 mg/100 gram) found on the stage of ripe fruit.
Inisiasi Kalus Daun Talinum triangulare (Jacq.) Willd pada Beberapa Kombinasi Konsentrasi Zat Pengatur Tumbuh 2,4-Dichlorophenoxyatic Acid dan Benzyl Adenine Anggraeni, Dinda; Ismaini, Lily; Surya, Muhammad Imam; Rahmi, Hayatul; Saputro, Nurcahyo Widyodaru
Agrikultura Vol 33, No 3 (2022): Desember, 2022
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v33i3.40540

Abstract

Ginseng Jawa (Talinum triangular) merupakan tanaman yang seluruh bagian tanamannya memiliki khasiat sebagai obat. Tujuan penelitian ini adalah optimalisasi zat pengatur tumbuh untuk inisasi kalus ginseng jawa. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Kebun Raya Cibodas – Badan Riset dan Inovasi Nasional (BRIN) pada bulan Agustus sampai bulan November 2021. Metode penelitian yang digunakan adalah metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan sembilan kombinasi perlakuan 2,4-D (Dichlorophenoxyatic Acid) and Benzyl Adenine (BA) dengan tiga ulangan. Eksplan daun berdiameter 8 mm ditanam pada botol kultur berisi media Murashige and Skoog yang mengandung kombinasi konsentrasi zat pengatur tumbuh 2,4-D (0,5 ppm, 1 ppm, dan 1,5 ppm) dan BA (0,5 ppm, 1 ppm, dan 1,5 ppm). Eksplan yang ditanaman mampu menghasilkan kalus dengan waktu awal muncul kalus antara 13-22 hari setelah inisiasi dan memiliki tekstur remah pada semua perlakuan. Terdapat empat warna kalus yang dihasilkan dari perlakuan ini, yaitu moderate yellow green, grayish yellow green, pale olive dan pale brown. Terdapat kombinasi konsentrasi terbaik diperoleh pada perlakuan D2B3 (1 ppm 2,4-D + 1,5 ppm BA) yang memberikan pertumbuhan optimal pada parameter panjang kalus sebesar 3,17 cm dan parameter berat basah kalus sebesar 2,16 gram serta berat kering kalus sebesar 0,21 gram.