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KUALITAS MADU LOKAL DARI LIMA WILAYAH DI KABUPATEN WONOSOBO Rofiqotul Khasanah; Sarjana Parman; Sri Widodo Agung Suedy
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Honey is sweet natural substance produced by honey bees (Apis sp.) from the nectar or other parts of plant. The nutritional content influences its quality. The contents are sugar, then water, and also some of organic acids. The purpose of this research is to analyse the honey quality from its water content, acidity, and sugar content in local honey from Wonosobo Regency. The samples were taken in the five locations, i.e Topengan village (TP), Pringapus (PA), Kalikuning (KK), Krinjing (KJ), and Mutisari (MS). The data analysis with ANOVA test (α = 5%). The Standardisation's reference of honey quality based on Indonesia National Standardisation (SNI) 2013 and EC Directive in 2001 year. The Results were water content was 22.17 - 23.67%, acidity was 34.08 - 39.37 ml NaOH/ kg, and sugar content was 74.83% - 76.17%. All samples were in low quality, except PA that was in standard with water content was 22,17%, acidity was 34,08 ml NaOH/kg, and sugar content was 76,17oBx. Keywords: honey quality, water content, acidity, and sugar content

Pengaruh Kombinasi Hormon Tumbuh Giberelin dan Auksin terhadap Perkecambahan Biji dan Pertumbuhan Tanaman Karet (Hevea brasiliensis Mull. Arg.) Kunta Adi Tetuka; Sarjana Parman; Munifatul Izzati
Jurnal Akademika Biologi Vol. 4 No. 1 Januari 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Rubber tree (Hevea brasilliensis Mull. Arg.) are commodities that have an important role in Indonesia. Gibberellin and auxin can accelerate seed dormancy breaking. The aim of this research was to study the effects of gibberellin and auxin on the germination and growth of Hevea brasilliensis Mull. Arg. This research was conducted in December 2014 until Pebruary 2015 in Bringin village, sub district of Bringin, Semarang Regency and Laboratory structure and function of plant biology, FSM Diponegoro University. The arrangment used is the full factorial pattern of random design (RAL) with two factors, namely the gibberellin (G0,G1,G2) and auxin (I0,I1,I2). This research using nine treatments, each of treatment are three replicates. Parameters were observed such as percent of germination, rate of germination, height of plant, number of leaves,length of the roots, wet weight, and dry weight. The analysis of data used is Analysist of Variances (ANOVA), if it shows significant results then continued with the test duncan’s multiple test (DMRT)in fact 95% level. The results showed that the hormone gibberellin 100 ppm to provide maximum results for improving germination percentage and germination rate Hevea brasilliensis Mull. Arg. Gibberellin100 ppm increasing germination percentage up to 28% and germination rate up to 45%. Combination of 200 ppm gibberellin and auxin 100 ppm to provide maximum results for plant height with the increase reached 61%, wet weight up to 100 % and dry weight up to 159%. Key words: Hevea brasilliensis Mull. Arg., gibberellic acid, indol-3-acetic acid, germination, growth.

Pengaruh Pemberian Ensim Amilase terhadap Kadar Bioetanol dari Limbah Sagu Padat Muhammad Lukman Hakim; Endah Dwi Hastuti; Sarjana Parman
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Sago starch processing industry in the village Plajan leaving solid waste that pollute the environment. Solid sago waste still contains carbohydrates ± 84.7%, which can be used as a base material for bioethanol production. Giving amylase enzyme in this study aims to optimize the starch hydrolysis process into glucose so as to increase the levels of bioethanol. The purpose of this study was to determine the effect of amylase enzyme with different volumes on levels of bioethanol. The research methods include hydrolysis, fermentation and distillation. The study design used was completely randomized design (CRD) with four treatments. Such treatment is A = 0 ml of α-amylase and glucoamylase 0 ml (control), B = 2 ml α-amylase and 2 ml of glucoamylase, C = 4 ml of α-amylase and glucoamylase 4 ml, D =6 ml of α-amylase and 6 ml glucoamylase three replications. Parameters observed that glucose levels, levels of ethanol, and the volume of bioethanol. Analysis of the data by Analysist Variance (ANOVA), followed by Duncan's Multiple Test Test (DMRT). These results indicate that administration of amylase enzyme is able to increase levels of ethanol until a certain volume. The most effective enzyme volume to produce the highest levels of bioethanol is the range of volume 2 ml α-amylase and 2 ml glucoamylase to 4 ml of α- amylase and 4 ml glucoamylase of ethanol grading 63.33% to 69.00% with a distillation process the distillation temperature between 70-90oC.Keywords: Amylase, bioethanol, sago solid waste.

Interaksi antara Tingkat Ketersediaan Air dan Varietas, Terhadap Kandungan Prolin serta Pertumbuhan Tanaman Kedelai (Glycine max L. Merr) Eko Wahono; Munifatul Izzati; Sarjana Parman
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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The Soybean production in Indonesia has decreased due to erratic weather conditions. It is necessary to plant soybeans that are tolerant to drought stress to overcome this problem. Soybean adapt to drought stress by accumulating proline to protect cells from damage. The purpose of this study was to determine the effect of water availability on growth and proline content in soybean. This study was conducted in Greenhouse Gombel Lama and Biology Laboratory Diponegoro University. The design used was a completely randomized design (CRD) with 2 factors these are the level of water availability and soybean plant variety. This research had 6 treatments and 3 replication. Data were analyzed using ANOVA, followed by Duncan's multiple range real difference test. Parameters measured were proline content and plant growth (plant height, fresh and dry weight of shoot and roots). The results show that the level of water availability affect the content of proline and growth. The lowest water availability had the highest prolin content, that was Grobogan variety with 2,15 µmol/gr of proline content while Wilis variety had 2.30 µmol/gr of proline content. The soybean plant variety not significantly affect the content of proline and plant growth, but significantly affect on plant height and number of leaves trifoliat. Grobogan variety was more adapted to drought stress than Wilis variety.Key words : proline, water availabyility, soybean, Glycine max

Pengaruh Konsentrasi dan Frekuensi Pemberian Pupuk Organik Cair (POC) Terhadap Pertumbuhan dan Produksi Bawang Merah (Allium ascalonicum L.). Moh. Khanif Nasuha; Sarjana Parman; Rini Budi Hastuti
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Shallots (Allium ascalonicum L) is a vegetable crop that has an important role in the economy of Indonesian society. This can be seen from the BPS (Central Bureau of Statistics) on onion consumption in the country in 2014 amounted to 935,000 tons. The high consumption of red onion cultivation resulted not memperhatiakan environmental factors are often farmers excessive use of inorganic fertilizers, resulting in soil becomes solid. Giving treatment Liquid Organic Fertilizer (POC) is expected to increase growth and yield of onion because the content of the complete haranya both micro and macro, and is also able to improve soil quality. The purpose of this research know the effect of the concentration, frequency and interaction of liquid organic fertilizer on the growth and production of onion (Allium ascalonicum L). The design used in this research was Randomized Complete Block Design (RCBD) factorial design with two factors, namelythe concentration of fertilizers and the frequency of fertilizer application. This study menggunakan6 treatment with a combination of concentration and frequency are K0F1 (0% 5 days), K0F2 (0% 10 days), K1F1 (25% 5 days), K1F2 (25% of 10 days), K2F1 (50% 5 days), K2F2 (50% of 10 days). Observations made during the 50 days after planting. Growth parameters measured were plant height and number of leaves, the production parameters measured were wet weight, dry weight, and the number of tubers. Data analysis using ANOVA test and further tests using test Duncan Multiple Test (DMRT) at 95% confidence level. The conclusion of this research is the concentration of liquid organic fertilizer (POC) significantly affect the growth parameters and onion production and the frequency and interaction no significant effect on growth parameters and onion production. Keywords: onion, organic fertilizer, concentration, frequency.

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