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Dewi Andayani Farmawati
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Chemistry

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PERBANDINGAN KUALITAS DNA DENGAN MENGGUNAKAN METODE BOOM ORIGINAL DAN BOOM MODIFIKASI PADA ISOLAT Mycobacterium tuberculosis 151 Dewi Andayani Farmawati; I Nengah Wirajana; Sagung Chandra Yowani
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.834 KB) | DOI: 10.24843/JCHEM.2015.v09.i01.p07

Abstract

Tuberculosis (TB) is a disease caused by the Mycobacterium tuberculosis. Isolation  DNA is a necessary step to obtain the bacterial chromosomal DNA used in the Polymerase Chain Reaction (PCR). Boom isolation method is an isolation method commonly used in isolation DNA of M. tuberculosis. In Bali, the isolation DNA of M. tuberculosis conducted at the Laboratory of Biomolecular FK UNUD uses boom modification method. This research aims to compare the quality of DNA produced by the Boom methods and Boom modification methods. This research was started with the isolation process using Boom method and Boom modification  and subsequently amplified by PCR. Detection of PCR products was performed with electrophoresis method. DNA quality was determined by the thickness of bands DNA PCR product and purity analysis by spectrophotometry UV-Vis. The results obtained show that the quality of DNA Mycobacterium tuberculosis 151  isolate using Boom modification method (Laboratory of Biomolecular FK UNUD) is relatively better than Boom original methods (Boom et al, 1990).
Co-Authors I Nengah Wirajana Sagun Chandra Yowani