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All Journal International Journal of Biosciences and Biotechnology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Planta Tropika Agroteknika Prosiding Seminar Nasional Program Pengabdian Masyarakat Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Jurnal Pengabdian Masyarakat dan Riset Pendidikan Research of Scientia Naturalis Pinisi Journal of Entrepreneurship Review
Nike Triwahyuningsih
Department Of Forestry, Faculty Of Forestry, Institut Pertanian INTAN Yogyakarta, Jl. Magelang Km 5,6 Yogyakarta
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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ISOLATION AND CHARACTERIZATION OF NOVEL ANTIBIOTIC-PRODUCING ACTINOBACTERIA FROM THE RHIZOSPHERE OF INDONESIAN MANGROVE FORESTS Triwahyuningsih, Nike; Mei, Chen; Schneider, Anna
Research of Scientia Naturalis Vol. 2 No. 5 (2025)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/scientia.v2i5.2567

Abstract

The rise of antimicrobial resistance is a global health crisis, demanding the urgent discovery of novel antibiotics. Indonesian mangrove forests, as a unique and underexplored ecosystem, represent a promising frontier for bioprospecting novel microorganisms. The plant rhizosphere, a zone of intense microbial activity, is particularly rich in actinobacteria, a phylum renowned for its prolific production of bioactive secondary metabolites. This research aimed to isolate and characterize novel antibiotic-producing actinobacteria from the rhizosphere of Indonesian mangrove plants. Rhizosphere soil samples were collected, and actinobacteria were isolated using selective media. All isolates were screened for antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans via the agar well diffusion method. The most potent isolate was subsequently characterized using morphological, biochemical, and 16S rRNA gene sequencing. From 72 distinct isolates, 15 displayed antimicrobial activity. One isolate, designated MGR-17, demonstrated exceptionally potent, broad-spectrum inhibition against all tested pathogens. Based on polyphasic taxonomy, MGR-17 was identified as a potentially novel species of the genus Streptomyces. In conclusion, the rhizosphere of Indonesian mangroves is a fertile source for discovering unique actinobacteria capable of producing novel antibiotics, and the discovery of Streptomyces sp. MGR-17 underscores this potential.
Strategic Development of Community-Based Tourism Entrepreneurship in Volcanic Ecotourism Destinations Triwahyuningsih, Nike; Kusmiyarti, Tati Budi; Noviastuti, Nina
Pinisi Journal of Entrepreneurship Review Vol. 4 No. 1 (2026): Pinisi Journal of Entrepreneurship Review
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62794/pjer.v4i2.219

Abstract

This study aims to formulate strategic directions for the development of community-based tourism entrepreneurship in a volcanic ecotourism destination by examining its internal and external conditions. The analysis employed the Internal Factors Analysis Summary, External Factors Analysis Summary, and SWOT framework to identify the main strategic priorities for sustainable destination development. Secondary data derived from visitor perceptions and management assessments were used to evaluate the destination’s strengths, weaknesses, opportunities, and threats. The findings indicate that internal strengths, particularly the attractiveness of the volcanic landscape and strong community participation, slightly outweigh existing limitations such as inadequate amenities and limited accommodation capacity. The external environment is also relatively favorable, supported by increasing demand for nature-based tourism and educational tourism activities, although challenges remain in the form of volcanic hazards and potential environmental degradation. The integration of IFAS, EFAS, and SWOT results highlights several strategic priorities, including geotourism development, community-based homestay expansion, hazard mitigation systems, and infrastructure improvement. This study contributes a context-sensitive strategic framework that integrates conservation, disaster risk awareness, and local entrepreneurship within community-based tourism development. The findings provide practical implications for policymakers, destination managers, and local communities in promoting sustainable ecotourism development in environmentally sensitive tourism areas.
Co-Authors Maulana, Alief Yahya Mei, Chen Noviastuti, Nina Pristiwantoro, Eko Cahyo Raharja, Kanda Rakhmi Zulhida, Isna Schneider, Anna Shafira, Amrina Yasmine Sunyata, Agus Syahidah, Tazkiyatul Tati Budi Kusmiyarti Utari, Lilik