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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal READ (Research of Empowerment and Development)
Atang Atang
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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IPerforma Larva Lalat Tentara Hitam (Hermetia illucens) sebagai Biokonversi Limbah Industri Pengolahan Carica Dieng (Vasconcellea pubescens) di Wonosobo Irma Fatmanintyas; Trisnowati Budi Ambarningrum; Atang Atang; Trisno Haryanto; Eko Setiyono
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p13

Abstract

Limbah berupa kulit dan biji yang berasal dari industri pengolahan carica Dieng (Vasconcellea pubescens) dapat menjadi permasalahan lingkungan apabila tidak tertangani dengan baik . Larva BSF (Hermetia illucens) sebagai agen biokonversi diharapkan mampu menjadi solusi untuk permasalahan tersebut. Penelitian dilakukan secara eksperimental dengan menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan dan 4 ulangan meliputi perlakuan kontrol, P1 = 100% kulit, P2 = 75% kulit + 25% biji, P3 = 50% kulit + 50% biji, P4 = 25% kulit + 75% biji, dan P5 = 100% biji. Waktu pengamatan penelitian dilakukan selama 21 hari. Parameter utama yang diamati adalah nilai konsumsi pakan dan indeks pengurangan limbah (WRI), sedangkan parameter pendukung berupa biomassa larva, diameter kapsul kepala dan tingkat kelulusan hidup (survival rate). Data yang diperoleh dianalisis menggunakan ANOVA dengan tingkat kesalahan 5%, dan apabila perlakuan berpengaruh nyata, dilanjutkan dengan uji DMRT dengan tingkat kesalahan 5%. Hasil penelitian dengan jenis pakan yang bervariasi menunjukkan nilai konsumsi pakan tercerna oleh larva BSF berkisar antara 60,42% - 81,26%. Sedangkan untuk nilai indeks pengurangan limbah (WRI) berkisar antara 2,82% - 3,73%. Nilai reduksi limbah lebih dari 50% menunjukkan adanya efektivitas larva BSF dalam mendegradasi limbah organik. Dengan demikian penggunaan jenis pakan berupa pakan penggunaan larva BSF efektif dalam mereduksi limbah pengolahan carica Dieng (V. pubescens).
Toksisitas Subletal Limbah Cair Batik Hasil Biosorpsi Terhadap Struktur Histologis Ginjal Ikan Mas (Cyprinus carpio) Khalil Ibrahim Sani; Sri Lestari; Atang Atang
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.261 KB) | DOI: 10.20884/1.bioe.2020.2.1.1769

Abstract

Histopathological studies related to the kidney of fish exposed to batik wastewater resulted from the biosorption process are necessary because the kidney is an target organ that plays an important role in maintaining water balance and osmoregulation. This research aims to determine the effect of batik wastewater resulted from the biosorption process exposure on kidney histology of Carp (Cyprinus carpio) and determine the concentration that has the most effect on it. The treatment consists of K0 (control) and three treatments of batik wastewater resulted from the biosorption process with different concentration from 25 %, 50 %, and 75 % LC50 96h, ie K1 1,32; K2 2,64, and K3 3,96 % v.v-1. The carp were exposed to batik wastewater resulted from the biosorption process for 7 days, and on the 8th day the fish was dissected, and the kidney was taken for histology process. The result of the research shows that exposure to batik wastewater resulted from the biosorption process with different concentrations give negative impact on the carp kidney such as tubular hypertrophy, tubular necrosis, and glomerular necrosis.
Pengaruh Antioksidan Alami pada Berat Testis Mencit (Mus musculus) yang Terpapar Senyawa Kimia Plastik Atang Atang; Endah Sri Palupi
Jurnal READ (Research of Empowerment and Development) Vol 1 No 2 (2020): Oktober 2020
Publisher : Pascasarjana Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.read.2020.1.2.3375

Abstract

Plastic is a material that is often found in everyday life, both for household needs, offices, agriculture/plantations, industry, etc. One type of plastic that is commonly used is polycarbonate plastic (polycarbonate / PC). The main ingredient in polycarbonate plastics is a compound 2,2-bis (4-hydroxyphenyl) propane known as bisphenol A (BPA). The purpose of this study was to determine the effect of natural antioxidants on the testicular weight of adult male mice exposed to the chemical compounds that make up the plastic. This study used a completely randomized design (CRD) with six treatments and five replications. The treatments included two control groups and four other groups treated with plastic boiled water and natural antioxidants. Plastic boiled water and antioxidants were given orally to 0.25 ml of male mice every day during the study. Quantitative data were analyzed using ANOVA and BNT. Based on the results of the research that has been carried out, it can be concluded that the provision of natural antioxidants for 21 days in mice (Mus musculus) exposed to plastic chemical compounds has an effect on decreasing the testicular weight of male mice in K (-) treatment / negative control. Based on data analysis through statistical tests that have been carried out, the series of natural antioxidant treatments for mice exposed to plastic chemical compounds for 21 days did not significantly affect the testicular weight of mice.
Histopatologi Hepar Mencit (Mus musculus) Jantan yang Diberi Air Rebusan Plastik terhadap Antioksidan Alami Nuridha Audinia Safitri; Atang Atang; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4606

Abstract

Plastic in Indonesian daily life cannot be avoided anymore. Materials for the manufacture of plastic can cause free radicals to the body, thus affecting the immune system. These materials are including phthalates and bisphenol-A (BPA), which commonly called as plasticizers. Free radicals in the body can be inhibited with antioxidants. Antioxidants can be obtained from endogenous (inside the body) and exogenous (outside the body). The purpose of this study was to determine the damage level of liver histopathological structure as the result of plastic boiled water induction towards natural antioxidants feeding on male mice (Mus musculus). This study used an experimental method with a completely randomized design (CRD) consisting of six treatments and five replications for 21 days. The treatments included two control groups and four other groups treated with plastic boiled water and natural antioxidants. The independent variable is natural antioxidants and the dependent variable is the liver histopathology of male mice. The qualitative parameters observed were the liver histopathological structure of male mice is in the state of inflammatory cell infiltration, degeneration, and necrosis. The research data is an overview of the liver histopathological structure of male mice. The obtained data were analyzed using descriptive qualitative analysis method. Oral induction of boiled-plastic water can affect the changes of liver histological structure. Natural antioxidants given in male mice can maintain the tissue structure as the result of boiled-plastic water induction. The results showed the damage level of male mice liver histopathological structure as reversible degeneration.
Reproduction Characteristics of Rice Field Eel (Monopterus albus Zuieuw) on Several Functionally Changed Lands in Banyumas Regency Priyo Susatyo; Nuning Setyaningrum; Elly Tuti Winarni; Titi Chasanah; Atang Atang
Journal of Tropical Life Science Vol. 8 No. 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.12

Abstract

Functional change of agricultural lands into non-agricultural lands will obviously have an impact on the dimin-ished or loss of habitat of the rice field eels (Monopterus albus Zuieuw). The aim of this research was to un-derstand the difference between (1) reproduction parameters (fecundity, gonadal maturity level (GML), and gonadal maturity index (GMI); (2) histological features of female and male gonadal development (oogenesis and spermatogenesis); (3) range of body length in each sexual phase; and (4) range of body length in each GML stage of captured eel from 4 sub-districts of Banyumas regency, Central Java (Ajibarang, Sumpyuh, Banyumas, and Sumbang). The total number of captured subjects were 631 eels; which consisted of 155; 227; 157; and 92 eels from Ajibarang, Sumpyuh, Banyumas, and Sumbang, respectively. We observed oogenesis in the stages of GML-1 (chromatin nucleolar stages and perinucleolar stage); GML-II (cortical alveolar formation stage or globular yolk stage), GML-3 (late globular yolk stage), GML-IV (mature or ripe stage), and follicle atretic stage. Also, we observed male gonadogenesis and spermatogenesis in the intersex. The range of GMI of captured eels was between 0.023 and 0.686. Eel in GML-I, GML-2, GML-3, and GML-IV had GMI within the range of 0.023-0.096; 0.096-0.425; 0.427-0.686; and >0.686, respectively. Eels achieved stage of GML-IV in body length range of 22.6-34.5 cm in Sumpyuh, 22.6-38.5 cm in Ajibarang, and 26.6-34.5 cm in Banyumas. There was no eel with the status of GML-IV in Sumbang, which had the most residential or functionally changed lands in its area. Based on these findings, can be concluded that the condition of rice fields or habitat of eel in functionally changed lands may affect eel’s growth and gonad maturation.
Co-Authors Eko Setio Wibowo Elly Tuti Winarni Endah Sri Palupi Haryanto, Trisno Irma Fatmanintyas Khalil Ibrahim Sani Nuning Setyaningrum Nuridha Audinia Safitri Priyo Susatyo Setiyono, Eko SRI LESTARI Titi Chasanah Trisnowati Budi Ambarningrum