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All Journal Buletin Udayana Mengabdi Jurnal Farmasi Udayana
M. A. Pratiwi
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Education Medicine & Pharmacology

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PEMBERDAYAAN MASYARAKAT PESISIR MELALUI PENINGKATAN SOFTSKILL DALAM PEMBUATAN SAMBAL TONGKOL N.P. P. Wijayanti; E. W. Suryaningtyas; D. A.A. Pebriani; I. K.W. Negara; M. A. Pratiwi; S. A. Saraswati
Buletin Udayana Mengabdi Vol 20 No 4 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.217 KB) | DOI: 10.24843/BUM.2021.v20.i04.p03

Abstract

Klungkung Regency, Bali has highly potential of marine fisheries, especially on capture fisheries. Capture fisheries potential was estimated 4.140,7 tons per year that consist of 2.898,2 tons of pelagic fishes and 1.242,5 tons of demersal fishes. Fishery products are a source of animal protein which is very beneficial for the body because they contain high protein and low fat. Furthermore, capture fisheries products are export commodities that can contribute for foreign exchange. Handling of fresh fishes is the most important part because it can affect the quality of fish product. Handling of fishery products is generally still on a traditional scale and is managed from generation to generation with limited capacity. “Pemindangan” processing in Kusamba Village, Dawan District, is one of the fish traditional handling techniques. Kusamba Village had very high production of Frigate Tuna, however not all Frigate Tuna can be processed with Pemindangan techniques. Thus, an alternative techniques for Frigate Tuna processing is necessary to improve the value of Frigate Tuna products. This products called “Sambal Tongkol”.The advantage of Sambal Tongkol are the manufacturing process does not require a long time and special expertise so that it can be adopted by local community in Kusamba Village. In addition, Sambal is one of favorite food products for Indonesian people as a staple food companion. Sambal products have been widely developed but not many have developed with the addition of Frigate Tuna. This community empowerment activity was expected to be able to improve soft skills of coastal communities which will be able to improve their economy as well.
Identifikasi Mutasi Gen rpoB Pada Daerah Hulu RRDR Mycobacterium Tuberculosis Multidrug Resistent Isolat P10 M. A. Pratiwi; K. Ratnayani; S.C. Yowani
Jurnal Farmasi Udayana Vol. 4, No. 1, Tahun 2015
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.754 KB)

Abstract

Multidrug Resistant Tuberculosis (MDR-TB) merupakan tuberculosis yang disebabkan oleh strain M.tuberculosis yang resistan sekurang-kurangnya terhadap obat antituberculosis lini pertama yaitu rifampisin dan isoniazid. Penelitian ini bertujuan untuk mengetahui mutasi dan perubahan asam amino pada isolat MDR-TB P10 fragmen daerah hulu RRDR (Rifampisin Resistance Determining Region) (125-421) Gen rpoB. Amplifikasi dilakukan dengan menggunakan metode Multiplex Polymerase Chain Reaction (Multiplex PCR) dengan menggunakan sepasang primer FrL2 5’CTAAGCTGC GCGAACCACTTGA3’ dan RevL2 5’TGATGAACTCGGCGGTGAC GAA3’. Produk PCR, disekuensing untuk mendapatkan urutan nukleotida. Analisis mutasi dilakukan dengan menggunakan program MEGA4. Dari penelitian yang dilakukan didapatkan bahwa metode multiplex PCR telah berhasil mengamplifikasi fragmen target yang berukuran 0,3kb dan 0,5kb. Proses sekuensing yang dilakukan menghasilkan pembacaan nukleotida sebanyak 254 basa. Analisis mutasi nukleotida menunjukkan bahwa ternyata pada isolat P10 tidak terdapat mutasi pada daerah hulu RRDR.
Co-Authors D. A.A. Pebriani Endang Wulandari Suryaningtyas I. K.W. Negara Ketut Ratnayani N.P. P. Wijayanti S. A. Saraswati Sagun Chandra Yowani