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Uji in Vitro Penghambatan Aktivitas Escherichia coli dengan Tepung Cacing Tanah (Lumbricus rubellus) H Julendra; A Sofyan
Media Peternakan Vol. 30 No. 1 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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This research was conducted to study the inhibition growth of E. coli by using earthworm (Lumbricus rubellus) meal. The earthworm meal was used in various concentrations, i.e. 0, 25, 50, 75 and 100 mg of earthworm meal in 100 ml DMSO for 0%, 25%, 50%, 75% and 100% (w/v) as treatments respectively. Data were analyzed by ANOVA in Randomized Complete Block Design. Duncan's multiple range test and polynomials orthogonal were used. Inhibition effects were measured through agar well diffusion test. Results showed that earthworm meal contain antibacterial compound which inhibit E. coli activity. There was a significant difference (P < 0.05) between earthworm meal treatments and control. The best antimicrobial effect was found in treatment of 50% (w/v) of earthworm meal and significantly higher than those of 25, 75 and 100% (w/v), but 25% (w/v) was not different (P > 0.05) with 75% (w/v). It is concluded that earthworm meal is capable to inhibit E. coli in-vitro at the optimum level of 50% (w/v). Key words: earthworm meal, E. coli, in-vitro, agar well diffusion

Inhibitory of Encapsulated Earthworm Extract (Lumbricus rubellus) on Pathogenic Bacteria in Vitro L Istiqomah; H Herdian; E Damayanti; S N Hayati; H Julendra
Media Peternakan Vol. 35 No. 1 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this study was to determine the inhibitory of earthworm (Lumbricus rubellus) extract (ECT) and encapsulated earthworm extract (ECT-t) as poultry feed additive against some pathogenic bacteria. Earthwom extract was prepared by dekokta method with water at 90 ºC then encapsulated by spray drying with maltodextrin as filler. In vitro antibacterial activity was performed using dilution method against Escherichia coli, Staphylococcus aureus, Salmonella pullorum, and Pseudomonas aeruginosa. The optical density results showed that started from ECT level 0.26% inhibited (PP. aeruginosa and S. aureus, while ECT level 0.52% inhibited (PE. coli and S. pullorum along with the increased levels of concentration. The percentage of growth showed that ECT level 1.04% had inhibitory (PE. coli and P. aeruginosa, while ECT level 0.52% showed antibacterial activity (PS. aureus. The result showed that S. aureus was the most sensitive bacterium to earthworms extract. ECT-t level 0.78% and 1.04% measured by spectrophotometer showed inhibitory (PP. aeruginosa and S. pullorum respectively. While ECT-t level 0.26% measured by spread plate count method showed inhibitory activity against P. aeruginosa. LD50 of E. coli and P. aeruginosa were found at ECT level 1.04%, while LD50 of S. aureus was found at level 0.52%. LD50 of P. aeruginosa was found at ECT-t level 0.52%. There were no antibacterial action (P>0.05) of ECT and ECT-t against S. pullorum.

Performance Enhancement and Immunity Profile of Broiler Treated Feed Additive Containing Lactic Acid Bacteria and Ganoderma lucidum A Sofyan; M Angwar; H Herdian; L Istiqomah; A Febrisiantosa; H Julendra; M H Wibowo; T Untari
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The experiment was conducted to evaluate the influence of feed additive containing lactic acid bacteria (LAB) and Ganoderma lucidum (GL) on body weight gain (BWG), feed efficiency (FE), performance index (PI), antibody titer (AT) against Newcastle disease and histopathology of broilers. Bacteria used were Lactobacillus salivarius and Pediococcus pentosaceus, which were isolated from broiler's intestine. A number of 195 unsexed day old chicks (Cobb strain) were arranged in a completely randomized design and consisted of 5 treatments, each in 3 equal replicates. The treatments were as followed T0: control/without-feed additive, T1: 1% LAB (109 cfu g-1), T2: 1% GL, T3: 1% of LAB 109 cfu g-1 + GL (1:1), T4: commercial antibiotic. Data were analyzed using ANOVA and conti-nued to Duncan's multiple range test. The results showed that T2, T3, T4 treatments significantly improved (P < 0.05) BWG, FE and PI of broilers. Broilers fed T3 had the highest PI, followed by T4, T1, T2 and T0. Broilers fed T3 had the highest AT value followed by T0, T2, T4, and T1. Histopathology profile showed that broiler fed T3 had no lesion on liver and intestine compared to others. The result of this experiment indicated that additive containing 0.25% L. salivarius, 0.25% P. pentosaceus, and 0.5% G. lucidum was able to enhance broiler performance.

Performance and Meat Quality of Broilers Infected with Escherichia coli and Administered with Bio Additive, Probiotic, and Antibiotic L Istiqomah; S N Hayati; E Damayanti; H Julendra; A A Sakti; T Untari
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

An experiment was conducted to determine the efficacy of bio additive administration (a mixture of Lumbricus rubellus extract, Morinda citrifolia leaves extract and lactic acid bacteria), probiotic, and antibiotic to the performance and meat quality of broiler infected with Avian Pathogenic Escherichia coli (APEC). In this study, 140 Jumbo 747 unsexed one-day old chicks were distributed randomly into 20 units of cages, each filled with 7 broilers. Twenty cages were assigned into 5 treatment groups, each treatment in 4 equal replicates. The treatments were as follows: A= E. coli infection (positive control), B= E. coli infection + bio additive, C= E. coli infection + probiotic, D= E. coli infection + antibiotic, E= No E. coli infection (negative control). A commercial corn-soybean-based broiler diet was formulated as the basal diets. The experimental period was 35 d and at 21st d of age the broilers were infected with E. coli except the E treatment. The result showed that bio additive administration (B) increased the final body weight (1,659.52 g) and body weight gain (1,616.81 g) and resulted in less FCR (1.87) among other treatments. The lowest mortality rate was recorded in B treatment (3.57%) and D treatment (3.57%). Probiotic (C treatment) and antibiotic (D treatment) decreased (P < 0.05) meat pH and tenderness compared to other treatments. Meanwhile bio additive administration did not affect the meat quality (pH, cooking loss, water-holding capacity, tenderness, and fat) compared to positive and negative controls. The lowest meat cholesterol content was observed in B treatment (54.02 mg/100 g). It is concluded that bio additive administration on broiler infected with E. coli increased the broiler performance and decreased the meat cholesterol compared to other treatments.

Bile Salt and Acid Tolerant of Lactic Acid Bacteria Isolated from Proventriculus of Broiler Chicken E Damayanti; H Julendra; A Sofyan; SN Hayati
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this research was to obtain the lactic acid bacteria (LAB) as probiotic candidates which have resistance to bile salt and acid condition. LAB was obtained using isolation method from proventriculus of broiler chicken. Selective MRS media with 0.2% CaCO3 addition were used for LAB isolation using pour plate sampling method under anaerobic condition. The result showed that four selected isolates had morphological and biochemical characteristics as LAB. The selected LAB was characterized as follow: antibacterial activities, antibiotic sensitivity, resistance on bile salt, gastric juice and acid condition, and biochemical identification. Antibacterial activities assay of cell free supernatant was confirmed using disc paper diffusion method which was arranged on factorial design and each treatment consisted of three replications. The cell free supernatant of LAB isolates had antibacterial activities against Escherichia coli, Pseudomonas aerugenosa, and Salmonella pullorum. Molecular identification procedure using 16S rRNA sequence analysis showed that R01 and R02 as Pediococcus acidilactici. The viability of the two isolates were tested by acid pH (pH 1, 2, and 3), gastric juice pH 2, and bile salt condition for digestives tract simulation. The result showed that R01 and R02 had a high viability percentages at pH 1, 2, and 3 (95.45%, 99.49%, 104.01%, and 67.17%, 120.74%, 103.4%, respectively) and at bile salt simulation for 1-2 hours (100.35%-102.71% and 100.02%-102.65%, respectively), but at gastric juice simulation for 1-2 hours, the P. acidilactici R01 had higher viability than P. acidilactici R02 (59.69%-76.53% versus 43.57%-40.69%, respectively). In the antibiotic sensitivity test for three antibiotics (i.e. erythromicin 15 µg, penicillin G 10 µg, and streptomycin 10 µg), the P. acidilactici R02 showed resistance to Streptomycin and Penicillin. It is concluded that P. acidilactici R01 and P. acidilactici R02 isolated from proventriculus of broiler chicken potential as probiotic candidates for chicken.

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