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All Journal Jurnal Veteriner Livestock and Animal Research

Daud Samsudewa

Department Of Animal Husbandry, Faculty Of Animal And Agriculture Sciences, University Of Diponegoro, Semarang, 50275

Author-ID : 2938032

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Identifikasi Gangguan Reproduksi Sapi Betina di Peternakan Rakyat (IDENTIFICATION OF REPRODUCTIVE DISORDERS IN FEMALE CATTLE AT LOCAL FARMS) Sutiyono Sutiyono; Daud Samsudewa; Alam Suryawijaya
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this research was to determine the female reproductive disorders in cattle reared by local farmers in the Distric of Kaliori, Rembang Regency, Central Java Province. A total of 94 cattle were used, in which had minimal one of each incisors had been replaced. The study used survey methods, and data were collected by interviewing with ranchers, rectal palpation, identification of the incisors, and body condition score of the cattle. In the implementation of the study, cattle were taken to a place determined by the chairman of the group of farmers (field or home page). The parameters of study were unheard of oestrus or not, the amount of artificial insemination, the number of incisors changed, body condition score of each cattle, the feed given, and their maintenance. The data were analyzed using statistical descriptive analysis on the mode, range, and percentage. The results showed that of the 94 cattle, which have disorders of reproductive activity as much as 80. Samples with impaired reproductive activity were divided into three groups. The first group was the old heifers that had no oestrus 25.00%, the second group was cattle that were more than three times applied artificial insemination and had not been pregnant 45.00%, and the third group was cattle that more than three months after the last giving birth had no oestrus 30.00%. The other reproductive disorders that occured in individual of the cattle was inactive ovaries (follicle undeveloped) 2.50%, 6.25% ovary hypofunction, ovarian cystic 1.25%, endometritis 2.50% and 2.50% abnormal uterus. In conclusion, the largest reproductive disorders in cattle caused by nutritional factors that provided by the farmers, and small disturbances due to some diseases and abnormal reproductive organs.

In Vitro Ovine Embryo Culture: Effectiveness of TCM-199 in Combination with 17?-Estradiol Hormone and Co-Culture of FTEC Yon Soepri Ondho; Dela Ayu Lestari; Daud Samsudewa
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Objective of this research was to determine the effectiveness of various culture mediums on thecleavage to morula in in vitro embryo culture of ovine. A total of 1.064 zygotes (fertilized oocytes) were grown in three kinds of different embryo culture medium, which were Tissue Culture Medium 199 (TCM-199) (T1), TCM-199 + 17â-estradiol (T2) and TCM-199 + co-culture of Fallopian Tube Epithelial Cell (FTEC) (T3). Data were analyzed using a completely randomized design with ANOVA analysis and HonestlySignificant Difference test. The variable observed were the percentage of embryo cleavage and morula. Results showed that T3 had the highest percentage (72.45%) of the cleavage occurred in zygotes cultured in the TCM-199 + FTEC co-culture medium, as well as embryo growth to the morula stage (75.75%). Incontrast, zygotes cultured in T1 and T2 had lower cleavage and lower morula development than T3, as much as 27.38%; 47.82% and 58.15%; 66.66%, respectively. In conclusion, TCM-199 + FTEC co-culture medium is the best medium for embryo culture.

Co-Authors Alam Suryawijaya Annisa Ayu Febrianti Dela Ayu Lestari, Dela Ayu Enny Tantini Setiatin Sally Saurma Silalahi Sutiyono Sutiyono Widya Yanuarista Yon Soepri Ondho

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