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Efektivitas Low Density Lipoprotein dan Kuning Telur Ayam dan Puyuh pada Pengawetan Semen Ayam Merawang (EFFECTIVESS OF LOW DENSITY LIPOPROTEIN AND EGG YOLK FROM CHICKEN AND QUAIL ON MERAWANG SEMEN PRESERVATION) Magfira Magfira; Raden Iis Arifiantini; Ni Wayan Karniani Karja; Sri Darwati
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.755 KB) | DOI: 10.19087/jveteriner.2017.18.3.345

Abstract

The successful of artificial insemination (AI) depends on the semen quality and extender. To minimize effect of cold shock during storage, extender is added with egg yolk. The objectives of this study were to compare the effectiveness of pure Low Density Lipoprotein (LDL) and egg yolk from domestic chicken and quail on motility and longevity of Merawang chicken sperm. The semen was collected by massage method from three Merawang roosters. Immediately after collection, semen was evaluated macroscopically and microscopically. Only semen demonstrated >70% motility and <20% sperm abnormality were used in this study. Semen divided into four aliquots and diluted with Lactate Ringer (LR) LDL chicken (RL-LDL-C), LR-LDL quail (LR-LDL-Q), LR- chicken Egg Yolk (LR-CEY), Ringer Lactate quail Egg Yolk (RL-QEY). Diluted semen than stored at 5oC. Sperm motility was examined twice a day and the longevity of sperm was determined every day until the sperm reach 0% motility. The motility of spermatozoa in the LR-LDL diluent differed from the sperm motility in the RL-QEY diluent at the 60th and 72th hour (P <0.05) poststorage. However, there was no difference in motility sperm in LR-LDL-C, RL-LDL-Q and RL-CEY. Additionally, there is no difference (P> 0.05) in spermatozoa longivity in the four diluents, with a range of longivities between 4.43 to 5.93 days. ABSTRAK Keberhasilan inseminasi buatan (IB) salah satunya bergantung pada kualitas semen dan pengencer yang digunakan. Dalam meminimalisir pengaruh cold shock saat penyimpanan, pengencer ditambahkan dengan kuning telur. Tujuan dari penelitian ini adalah untuk membandingkan efektivitas Low Density Lipoprotein (LDL) dan kuning telur yang berasal dari ayam kampung dan puyuh terhadap motilitas dan longivitas spermatozoa ayam. Koleksi semen dilakukan menggunakan metode pemijatan pada tiga ekor ayam merawang. Setelah semen dikoleksi, selanjutnya semen dievaluasi secara makroskopis dan mikroskopis. Semen yang menunjukkan motilitas 70% dan abnormalitas kurang dari 20% dibagi empat dan diencerkan menggunakan Ringer Laktat-LDLA (RL-LDLA), Ringer Laktat-(RL-LDLP), Ringer Laktatkuning telur ayam (RL-KTA), dan RL-kuning telur puyuh (RL-KTP). Semen yang telah diencerkan kemudian disimpan pada suhu 5oC. Motilitas spermatozoa diamati dua kali sehari sampai motilitas mencapai 0%. Motilitas spermatozoa dalam pengencer RL-LDLA berbeda dengan motilitas spermatozoa dalam pengencer RL-KTP pada jam ke-60 dan ke-72 (P<0.05) pascapenyimpanan. Akan tetapi tidak terdapat perbedaan motilitas spermatozoa dalam RL-LDLA, RL-LDLP dan RL-KTA. Longivitas spermatozoa dalam empat pengencer tidak terdapat perbedaan (P>0.05) dengan rentang longivitas antara 4,43 sampai 5,93 hari.
PENGARUH EKSTRAK METANOL DAUN PEPAYA (Carica papaya L.) TERHADAP AKTIVITAS ENZIM LIPASE Nurhaeni Nurhaeni; Ahmad Ridhay; Magfira Magfira
KOVALEN: Jurnal Riset Kimia Vol. 3 No. 3 (2017): Edisi Desember
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.129 KB)

Abstract

Has conducted research on the effects of methanol extract of leaves papaya (Carica papaya L.) Against Lipase Enzyme Activity. This study aimed to obtain information in a methanol extract inhibits lipase activity. The method used is Complete Random Design (RAL), with goal attainment is done by determining the incubation time lipase enzyme, pH optimum lipase enzyme, and maximum substrate concentration lipase enzyme. And determining the effect of the methanol extract to inhibit the lipase enzyme activity. The results showed that the best incubation time of lipase obtained by time of 120 minutes, the optimum pH 7 and the maximum substrate concentration of 4%. With the highest activity were obtained 16.94 mol /ml.menit, 13.33 mol / ml.menit, and 12.89 mol / ml.menit. The methanol extract of papaya leaves can inhibit the lipase enzyme activity the best concentration of 2% with the acquisition activity of 3.67 mol/ml.menit.Keyword: papaya, lipase, incubation time, optimum pH, substrate concentration.