Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Jurnal Veteriner
Hasbi .
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Veterinary

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Tingkat Pematangan Inti Oosit Domba dan Pembentukan Pronukleus Setelah Parthenogenesis dengan Penambahan Glutathione (NUCLEAR MATURATION RATE OF OVINE OOCYTES AND PRONUCLEAR FORMATION AFTER PARTHENOGENESIS WITH GLUTATHIONE ADDITION) Hasbi .; Sri Gustina; Mohamad Agus Setiadi; Iman Supriatna
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.381 KB)

Abstract

The aim of this study was to investigate the nuclear maturation rate of ovine oocytes and pronuclearformation following parthenogenesis with glutathione (GSH) addition in maturation and culture medium.In the first experiment, acolytes were matured in tissue culture medium (TCM) 199 with 0 (control), 0.25,0.5 and 1 mM glutathione (GSH) addition. In the second experiment, oocytes were matured in maturationmedium, then parthenogenetically activated by exposing to 7% ethanol (v/v) for 7 min, followed by treatmentwith 5 ìg/ml cytochalasin B for 4 h. Oocytes then cultured in medium TCM 199 + 10% FBS with treatmentswithout addition of 1 mM GSH (T0), addition in maturation medium (T1), addition in culture medium (T2),and addition in both maturation and culture medium (T3) then incubated at 38,5oC with 5% CO2 for 20-24h. The results showed that, nuclear maturation rate was not significantly different (P>0.05) among fourtreatments. The percentage of oocytes reached metaphases II (MII) stage were 79.71%, 79.07%, 80.95%and 84.13%, respectively. Percentages of activated oocyte with T1 (65.31%) and T3 (67.27%) were higher(P<0.01) compared to T0 (46.81%) and T2 (54.35%). However, T3 was not significantly different with T1. Inconclusion, the addition of GSH in maturation medium could not improve nuclear maturation rate butmore effective in supporting the number of activated oocytes.
Co-Authors Iman Supriatna M Agus Setiadi Sri Gustina