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Tingkat Pematangan Inti Oosit Domba dan Pembentukan Pronukleus Setelah Parthenogenesis dengan Penambahan Glutathione (NUCLEAR MATURATION RATE OF OVINE OOCYTES AND PRONUCLEAR FORMATION AFTER PARTHENOGENESIS WITH GLUTATHIONE ADDITION) Hasbi .; Sri Gustina; Mohamad Agus Setiadi; Iman Supriatna
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.381 KB)

Abstract

The aim of this study was to investigate the nuclear maturation rate of ovine oocytes and pronuclearformation following parthenogenesis with glutathione (GSH) addition in maturation and culture medium.In the first experiment, acolytes were matured in tissue culture medium (TCM) 199 with 0 (control), 0.25,0.5 and 1 mM glutathione (GSH) addition. In the second experiment, oocytes were matured in maturationmedium, then parthenogenetically activated by exposing to 7% ethanol (v/v) for 7 min, followed by treatmentwith 5 ìg/ml cytochalasin B for 4 h. Oocytes then cultured in medium TCM 199 + 10% FBS with treatmentswithout addition of 1 mM GSH (T0), addition in maturation medium (T1), addition in culture medium (T2),and addition in both maturation and culture medium (T3) then incubated at 38,5oC with 5% CO2 for 20-24h. The results showed that, nuclear maturation rate was not significantly different (P>0.05) among fourtreatments. The percentage of oocytes reached metaphases II (MII) stage were 79.71%, 79.07%, 80.95%and 84.13%, respectively. Percentages of activated oocyte with T1 (65.31%) and T3 (67.27%) were higher(P<0.01) compared to T0 (46.81%) and T2 (54.35%). However, T3 was not significantly different with T1. Inconclusion, the addition of GSH in maturation medium could not improve nuclear maturation rate butmore effective in supporting the number of activated oocytes.
PENINGKATAN HASIL BELAJAR IPS TEMA PERISTIWA DALAM KEHIDUPAN MENGGUNAKAN METODE PEMBELAJARAN SCRAMBLE PADA SISWA KELAS V UPT. SD NEGERI 20 BARINGIN Sri Gustina
Ensiklopedia of Journal Vol 3, No 3 (2021): Vol 3 No 3 Edisi 1 April 2021
Publisher : Lembaga Penelitian dan Penerbitan Hasil Penelitian Ensiklopedia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.44 KB) | DOI: 10.33559/eoj.v3i3.730

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This study aims to determine the increase in social studies learning outcomes the theme of events in life using the scramble learning method for class students V UPT. SD Negeri 20 Baringin sub-district Lima kaum. This type of research is classroom action research (PTK). The PTK design used Kemmis and Taggart which included planning, action and observation, and reflection. Data collection techniques using tests. The data analysis technique uses descriptive analysis to analyze student learning outcomes. The research subjects were class students V UPT.SD Negeri 20 Baringin which amounted to 20 students. The criterion for success is at least 85% of the total number of students achieving the Minimum Completion Criteria (KKM) that the school has set, namely the value of 80. The results show that there is an increase in social studies learning outcomes Themes of Events in Class Student V UPT. SD Negeri 20 Baringin by using the scramble learning method. This can be seen from the increase in students' social studies learning outcomes (Themes of Events in Life) from pre-action, cycle I, and cycle II. In the pre-action, the students 'learning completeness was obtained by 50%, the first cycle was 72.22%, and in the second cycle, the students' learning completeness was obtained by 86.11%. The increase in student learning outcomes from pre-action to cycle I was 22.22% and from cycle I to cycle II was 13.89%. While the average value in pre-action was 66.67 to 77.5 in cycle I and to 82.5 in cycle II So, the scramble learning method can improve the social studies learning outcomes of class V students UPT. SD Negeri 20 Baringin.
PENINGKATAN HASIL BELAJAR IPA TEMA LINGKUNGAN SAHABAT KITA MATERI SIKLUS AIR MENGGUNAKAN MODEL INQUIRY DAN MEDIA DIORAMA PADA PESERTA DIDIK KELAS V UPT. SD NEGERI 20 BARINGIN Sri Gustina
Ensiklopedia of Journal Vol 3, No 4 (2021): Vol 3 No. 4 Edisi 2 Juli 2021
Publisher : Lembaga Penelitian dan Penerbitan Hasil Penelitian Ensiklopedia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.777 KB) | DOI: 10.33559/eoj.v3i3.821

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Penelitian ini dilaksanakan untuk meningkatkan hasil belajar IPA pada peserta didik kelas V UPT. SD Negeri 20 Baringin tahun pelajaran 2020/2021 yang dilatar belakangi karena dalam pembelajaran IPA dirasa cukup sukar maka perlu adanya model dan media yang bervariasi sehingga kemampuan untuk mencapai Kriteria Ketuntasan Minimal dapat terpenuhi. Rumusan masalah ini adalah apakah model Inquiry dan Media Diorama dapat meningkatkan hasil belajar IPA materi Siklus Air pada Peserta didik kelas V UPT. SD Negeri 20 Baringin tahun pelajaran 2020/2021? Penelitian ini merupakan penelitian tindakan kelas. Subjek dari penelitian ini adalah siswa kelas V UPT. SD Negeri 20 Baringin tahun pelajaran 2020/2021. Jumlah peserta didik yang menjadi subjek penelitian ini adalah 35 peserta didik yang terdiri atas 19 laki-laki dan 16 perempuan. Instrumen penelitian meliputi RPP, lembar observasi guru, lembar observasi peserta didik, dan tes evaluasi. Metode pengumpulan data yang digunakan yaitu tes, observasi dan dokumentasi. Berdasarkan hasil penelitian di UPT. SD Negeri 20 Baringin tahun 2021 model Inquiry dan media Diorama dapat meningkatkan hasil belajar IPA materi siklus Air. Hal ini dibuktikan dari persentase hasil belajar yang meningkat dari Siklus I ke Siklus II. Siklus I jumlah peserta didik yang tuntas belajar ada 26 (74,3%) peserta didik dan yang belum tuntas 9 (25,7%). Siklus II terdapat 31 (88,6%) peserta didik. Berdasarkan data tersebut, hasil belajar peserta didik kelas V UPT. SD Negeri 20 Baringin setelah dilakukan penelitian mengalami peningkatan siklus I 74,3% dan siklus II 88,6%. Peningkatan yang diperoleh dari siklus I (74,3%) ke siklus II (88,6%) adalah 14,3%.
PENGARUH TERAPI KETAWA TERHADAP TEKANAN DARAH LANSIA HIPERTENSI DI WILAYAH KERJA PUSKESMAS PEMBANTU DESA KEPENUHAN RAYA Gustina, Sri; Herma Yesti; Andriana
Maternity and Neonatal : Jurnal Kebidanan Vol. 12 No. 1 (2024): Maternity and Neonatal : Jurnal Kebidanan
Publisher : Fakultas Ilmu Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30606/jmn.v12i1.2534

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Hypertension is a disease caused by a condition when blood pressure at 130/80 mmHg or more for which the cause is not known with certainty. The elderly with hypertension need attention, especially in reducing blood pressure. Nonpharmacological action, laughter therapy can lower blood pressure. Laughter can lower levels of certain hormones linked to the stress response: cortisol, epinephrine and dopac. Laughter relieves stress, depression, anxiety, sadness, anger and can trigger the release of endorphins, thereby relieving pain. This research is a pre-experimental study conducted with a sample of 13 respondents. The results of this study indicate that the average decrease in blood pressure in elderly people with hypertension before and after being given laughter therapy is 15.385 mmHg for systolic blood pressure and 7.69 for diastolic blood pressure. Asymp.Sig value is 0.043 which means <0.05 Ha is accepted and Ho is rejected. There is an effect of laughter therapy on blood pressure in the elderly with hypertension in the working area of the Kepuhan Raya village auxiliary health center in 2023.
Motilitas dan Viabilitas Spermatozoa Sapi Bali Polled dan Bertanduk pada Setiap Tahapan Proses Pembekuan Gustina, Sri; Hasbi, Hasbi; Sonjaya, Herry; Baco, Sudirman; Toleng, Abdul Latief; Mutmainna, Mutmainna; Farida, Sitti
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.01 KB) | DOI: 10.33772/jitro.v9i1.18417

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ABSTRAKSapi bali merupakan sapi yang dikembangkan, dimanfaatkan dan dilestarikan sebagai sumberdaya ternak asli. Saat ini telah dikembangkan populasi sapi bali polled. Sapi bali polled merupakan sapi bali yang tanduknya tidak bertumbuh secara alami. Tujuan dari penelitian ini adalah untuk mengetahui persentase motilitas dan viabilitas spermatozoa sapi bali polled dan bertanduk pada setiap tahapan proses pembekuan. Koleksi semen dilakukan dengan metode vagina buatan. Semen hasil koleksi selanjutnya dievaluasi secara makroskopik dan mikroskopik. Semen yang memenuhi persyaratan diproses lebih lanjut untuk dibekukan. Pejantan yang digunakan sebanyak 3 ekor polled dan 3 ekor bertanduk dengan umur 3,5 sampai 6 tahun. Parameter yang diamati meliputi motilitas dan viabilitas selama proses pembekuan sampai post thawing. Data yang diperoleh diuji dengan uji-t (sampel T-Test independen). Hasil penelitian menunjukkan bahwa tidak ada perbedaan nyata (p>0,05) antara sapi bali polled dan bertanduk terhadap persentase motilitas setelah pengenceran (63,33±1,92 vs 65,00±0,00). Namun, terdapat perbedaan yang nyata (p<0,05) setelah equilibrasi yaitu 54,99±1,35 vs 60,00±0,00 dan post thawing 45,41±0,83 vs 49,58±0,83. Persentase viabilitas setelah pengenceran dan setelah equilibrasi tidak berbeda nyata (p>0.05) yaitu 81,94±4,05 vs 86,51±1,26 dan 72,80±6,80 vs 80,17±2,74. Namun, pada post thawing terdapat perbedaan yang nyata (p<0,05) yaitu 56,95±3,74 vs 72,55±2,00. Kesimpulannya bahwa persentase motilitas dan viabilitas spermatozoa sapi bali polled lebih rendah dibandingkan yang bertanduk.Kata Kunci: motilitas, sapi bali polled, sapi bali bertanduk, spermatozoa, viabilitasMotility and Viability of Spermatozoa of Polled and Horned Bali Bulls at Each Stage of the Freezing Process ABSTRACTBali cattle are cows and bulls that are developed, utilized and preserved as a native livestock resource. Currently, a population of polled bali cattle has been developed. Polled bali cattle are bali cattle whose have horns do not grow naturally. The purpose of this study was to determine the percentage of motility and viability of spermatozoa of polled and horned bali cattle at each stage of the freezing process. Semen collection was carried out by the artificial vaginal method. The collected semen was then evaluated macroscopically and microscopically. The semen that meets the requirements is further processed to be frozen. The bulls used were 3 polleds and 3 horneds with an age of 3.5 to 6 years. Parameters observed included motility and viability during the freezing process until post thawing. The data obtained were tested by t-test (independent sample T-Test). The results of this study indicated that there was no significant difference (p>0.05) between polled and horned bali cattle on the percentage of motility after dilution (63.33 ± 1.92 vs 65.00 ± 0.00). However, there was a significant difference (p<0.05) after equilibration (54.99±1.35 vs 60.00±0.00), and post thawing (45.41±0.83 vs 49.58±0.83). The percentage of viability after dilution and after equilibration was not significantly different (p>0.05), 81.94 ± 4.05 vs 86.51 ± 1.26 and 72.80 ± 6.80 vs 80.17 ± 2.74. However, there was a significant difference in post thawing (p<0.05), 56.95 ± 3.74 vs 72.55 ± 2.00. It can be concluded that the percentage of motility and viability of spermatozoa of polled bali cattle is lower than horned.Keywords: motility, polled bali bull, horned bali bull, spermatozoa, viability
TANGGUNG JAWAB HUKUM DAN EKONOMI DALAM PERLINDUNGAN DATA PRIBADI DI ERA DIGITAL Zainudin Hasan; Salsabila Tiara Putri; Sri Gustina; Ahmad Rifki Satria; Kevin Oksandy Ramadhani; Muhammad Satrio
Causa: Jurnal Hukum dan Kewarganegaraan Vol. 7 No. 12 (2024): Causa: Jurnal Hukum dan Kewarganegaraan
Publisher : Cahaya Ilmu Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.3783/causa.v7i12.7183

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Perlindungan data pribadi di era digital telah menjadi isu krusial seiring dengan pesatnya perkembangan teknologi dan maraknya transaksi online. Penelitian ini membahas tanggung jawab hukum dan ekonomi pelaku usaha dalam melindungi data pribadi konsumen, serta dampak dari pelanggaran data terhadap bisnis. Pelaku usaha memiliki kewajiban hukum untuk menjaga kerahasiaan dan keamanan data konsumen, yang diatur dalam berbagai regulasi nasional, seperti Undang-Undang Perlindungan Data Pribadi. Selain itu, dari perspektif ekonomi, pelanggaran data dapat menyebabkan kerugian finansial yang signifikan, seperti denda, kehilangan kepercayaan konsumen, dan biaya pemulihan data.Penelitian ini juga menyoroti tantangan yang dihadapi pelaku usaha dalam menerapkan regulasi perlindungan data pribadi, termasuk tingginya biaya yang diperlukan untuk keamanan siber. Kesimpulan penelitian ini menekankan perlunya peningkatan regulasi dan pengawasan oleh pemerintah serta pentingnya kepatuhan pelaku usaha terhadap regulasi yang berlaku. Saran bagi konsumen adalah untuk lebih sadar akan pentingnya perlindungan data pribadi dan hak-hak mereka dalam bertransaksi online.
The Effect of Complementary Therapy from Plant Extracts on Breast Milk Production Farlikhatun, Lili; Rosyidah, Fitri Ummu; Karmilasari, Novia; Seftaliana; Masfupah; Gustina, Sri; Rukmini
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.8823

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Breastfeeding issues arise when mothers cannot produce enough milk to meet their babies' nutritional needs. This study aims to assess the effect of complementary therapies, such as consuming moringa leaves, katuk leaves, soy milk, spinach, young papaya, and using lavender aromatherapy, on increasing breast milk production. The quasi-experimental study involved postpartum mothers on day 4, using a pre-test and post-test design with six variables. Results showed that 83.9% of 56 mothers who consumed moringa leaves, 84.5% of 58 mothers who drank soy milk, 81.3% of 48 mothers who ate katuk leaves, 86.4% of 44 mothers who consumed spinach, 82.7% of 52 mothers who ate young papaya, and 83.3% of 30 mothers who used lavender aromatherapy experienced sufficient breast milk production. These findings demonstrate that all six therapies positively influence breast milk production. It is recommended that healthcare providers routinely offer these complementary therapies to postpartum mothers to support exclusive breastfeeding for their babies.
Tindak Pidana Judi Online : Penegakan Hukum Oleh Kepolisian, Serta Upaya Dan Strategi Penanganannya Sri Gustina; Alfarel Kurniawan; Yusril Pandawa
Jurnal Intelek Insan Cendikia Vol. 2 No. 5 (2025): MEI 2025
Publisher : PT. Intelek Cendikiawan Nusantara

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Abstract

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Kualitas Spermatozoa Sapi Bali Polled setelah Pemberian Pakan Tersuplementasi Taoge (Phaseolus radiatus L.) Gustina, Sri; Hasbi, Hasbi; Sonjaya, Herry; Baco, Sudirman; Qhatimah, Husnul; Saputra, Wandi; Mutmainna, Mutmainna; Kaiin, Ekayanti Mulyawati; Maulana, Tulus
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86154

Abstract

            The study aimed to increase the sperm quality of polled Bali bulls with the supplementation of bean sprout (Phaseolus radiatus L.) in feeding. This research was used two polled Bali bulls aged 5-6 years old and supplemented with bean sprout 1 kg/bull two times in a week for two months. Semen was collected using artificial vagina method. The evaluation macroscopic of semen was volume while the microscopic evaluation such as motility, viability, abnormality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation. The result of this research showed that semen volume and spermatozoa abnormality of fresh semen after feeding of bean sprouts were not significantly difference (P>0.05) compared to before feeding, but motility and viability were significantly higher (P<0.01). Motility and acrosome integrity of frozen semen were not significantly difference (P>0.05) while viability and PMI were significant higher (P<0.01), abnormality and DNA Fragmentation were significant lower (P<0.01) after feeding compared to before. The research revealed that the supplementation of bean sprouts could increase the motility and viability but had no effect on volume and abnormality of fresh semen. Meanwhile in frozen semen, it increased viability and PMI and decreased abnormality and DNA fragmentation but had no effect on motility and acrosome integrity.
Performa Estrus dan Hormon Estrogen Sapi Friesian Holstein Postpartus yang Diberi Urea Multinutrient Molasses Block (UMMB) dengan Perekat Tepung Tapioka Hasbi, Hasbi; Ramadan, Zyahrul; Utamy, Renny Fatmyah; Ako, Ambo; Masturi, Masturi; Gustina, Sri; Tasya, Tasya; Mufliha, Rara; Nurbina, Andi Fitri; Mutfaidah, Andi; Saputra, Rio; Mahayani, I Dewa Ayu; Mutmainna, Mutmainna; Rahman, Andi Arif; Sukri, Siti Annisa
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.87348

Abstract

The reproductive ability of Holstein Friesian (FH) cows greatly affects milk yield because, however, in the lactation period, dairy cows anestrus postpartus, which is the condition where does not appear in estrus postpartus. Anestrus postpartus to uterine involution as a consequence of nutritional deficiencies. Recovery from can be this condition can be conducted by additional feeding. One of the supplementary feeds is urea multinutrient molasses block (UMMB). UMMB consists of fillers and adhesives and cement is the most used in UMMB production as an adhesive. UMMB is an additional feed using cement dairy cows health problems. Therefore, the objective of this research was to determine the effect of cement substitution with tapioca meal as an adhesive on postpartus estrus of FH cows. A total of 9 lactating FH dairy cows were divided into 3 dietary groups. Each treatment had 3 cows as replicates. The dietary treatments consisted of UMMB with 100% cement adhesive (as D0); 50% cement and 50% tapioca (D1); and 0% cement and 100% tapioca meal (D2). The parameters were postpartus estrus, estrus speed, estrus duration, estrus intensity, and estrogen hormone levels. The results revealed that the treatment had a significant effect (P<0.05) on the duration of estrus, while it had no significant effect (P>0.05) neither on postpartus estrus, estrus speed, estrus intensity, or estrogen hormone levels. Therefore, it can be concluded that tapioca meals can be used as a substitute for cement as much as 50% provides as an adhesive in UMMB production for better estrus performance.