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Roza Helmita
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Kajian In vitro Aktivitas Sel-Sel Trofoblas Blastosis Mencit Aging dan Pengaruhnya terhadap Kegagalan Implantasi Ita Djuwita; Roza Helmita; Adi Winarto; Wahyudin -
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1932.848 KB)

Abstract

The objectives of this in vitro study were to investigate the hatching rate, the outgrowth diameter andthe activity of mitochondria Nicotiamide Adenin Dinucleotide Dyhidrogenage (NADH)-CoQ reductase ofblastocysts trophoblast cells from aging mice. Blastocysts of aging (age >12 months) and young productive(age 2 months) mice were collected from the cornua utery at day-4 of pregnancy and were cultured inmDMEM medium supplemented with 10% New Born Calf Serum (NBCS), 10% ITS, and 50 ?g/mlgentamicine, in 5% CO2 incubator at 37°C for 10 days. The blastocysts hatching rate and the trophoblastsmonolayer were examined for their diameter outgrowth and the NADH-CoQ reductase activity. The resultsshowed that the hatching rate, the trophoblast outgrowth diameter and the activity of NADH-CoQ reductaseof blastocysts collected from productive mice were significantly higher than those collected from the agingmice (P<0,05). It can be concluded that the impairment of blastocysts implantation especially, in agingmice were caused by the low activity of the NADH-CoQ reductase that play important role in energyproduction needed for the hatching and trophoblast outgrowth.
Transformasi Limbah Kulit Bawang Menjadi Pupuk Organik Cair: Pemberdayaan Siswa SMP N 3 Salimpaung dalam Pengelolaan Lingkungan Berbasis Edukasi Roza Helmita; Fitri, Rama Eka
PRAXIS: Jurnal Pengabdian kepada Masyarakat Vol. 3 No. 2 (2024): PRAXIS
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47776/praxis.v3i2.1424

Abstract

Nagari Salimpaung, located near Mount Marapi, is characterized by its cool and fertile environment, making it highly suitable for agriculture, particularly vegetable farming. However, agricultural and household activities in the region generate a significant amount of organic waste, including onion peels, which are often underutilized and contribute to environmental pollution. A viable solution to address this issue is converting the waste into liquid organic fertilizer (LOF) through a fermentation process. This community engagement activity was conducted by students of UIN Mahmud Yunus Batusangkar during their community service program (KKN) on November 5, 2024, at SMPN 3 Salimpaung. A Community-Based Research (CBR) approach was applied, involving the school community in every stage, from problem identification to evaluation. The activity consisted of three stages: (1) Planning, which included discussions with teachers and students to identify environmental issues and select suitable solutions; (2) Implementation, which involved educating participants on organic waste management, demonstrating the LOF production process, and facilitating hands-on practice by students; and (3) Evaluation, which encompassed monitoring the fermentation process and assessing the quality of the resulting fertilizer. The results indicated that the eighth-grade students successfully participated in the socialization and independently produced liquid organic fertilizer. The fertilizer showed potential for application in local agriculture and home gardening. This activity not only enhanced students’ understanding of organic waste management but also equipped them with practical skills for sustainable environmental practices. The program is expected to serve as a starting point for broader waste management initiatives in Nagari Salimpaung.
Transformasi Limbah Kulit Bawang Menjadi Pupuk Organik Cair: Pemberdayaan Siswa SMP N 3 Salimpaung dalam Pengelolaan Lingkungan Berbasis Edukasi Roza Helmita; Fitri, Rama Eka
PRAXIS: Jurnal Pengabdian kepada Masyarakat Vol. 3 No. 2 (2024): PRAXIS
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47776/praxis.v3i2.1424

Abstract

Nagari Salimpaung, located near Mount Marapi, is characterized by its cool and fertile environment, making it highly suitable for agriculture, particularly vegetable farming. However, agricultural and household activities in the region generate a significant amount of organic waste, including onion peels, which are often underutilized and contribute to environmental pollution. A viable solution to address this issue is converting the waste into liquid organic fertilizer (LOF) through a fermentation process. This community engagement activity was conducted by students of UIN Mahmud Yunus Batusangkar during their community service program (KKN) on November 5, 2024, at SMPN 3 Salimpaung. A Community-Based Research (CBR) approach was applied, involving the school community in every stage, from problem identification to evaluation. The activity consisted of three stages: (1) Planning, which included discussions with teachers and students to identify environmental issues and select suitable solutions; (2) Implementation, which involved educating participants on organic waste management, demonstrating the LOF production process, and facilitating hands-on practice by students; and (3) Evaluation, which encompassed monitoring the fermentation process and assessing the quality of the resulting fertilizer. The results indicated that the eighth-grade students successfully participated in the socialization and independently produced liquid organic fertilizer. The fertilizer showed potential for application in local agriculture and home gardening. This activity not only enhanced students’ understanding of organic waste management but also equipped them with practical skills for sustainable environmental practices. The program is expected to serve as a starting point for broader waste management initiatives in Nagari Salimpaung.
Co-Authors Adi Winarto Fitri, Rama Eka Ita Djuwita Wahyudin -