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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Prosiding Seminar Nasional Kusuma
Siti Gusti Ningrum
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Veterinary

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Cross Reaction of Serum in Salmonella enteritidis- Vaccinated Chicken to Some Salmonella enterica Serotypes (REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) Wyanda Arnafia; Siti Gusti Ningrum; Erfiandini Eka Puspita; Denny Widaya Lukman; Fachriyan Hasmi Pasaribu; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.699 KB)

Abstract

Salmonella spp. has been recognized as the major cause of food-borne illness in humans worldwidecausing remain relevant to public health. Poultry vaccination is one promising strategy to mitigateSalmonella infection in poultry and, in turn, in humans as well. The objective of this study was to assessthe potential of cross-reaction of serum in Salmonella enteritidis-vaccinated chicken to some serotype ofSalmonella enterica. Four female, Isa Brown layer chickens (20 weeks old), were vaccinated with S. enteritidisstrain Sm24/Rif12/Ssq (intra vena) to induced the production of specific antibodies in serum. Crossreactionof serum in S. enteritidis-vaccinated chicken were assess with agar gel immunodiffusion test(AGID) with S. enteritidis, S. pullorum, S. typhimurium, S. typhi, and Escherichia coli antigens. Serumcould react with S. enteritidis and all types of S enterica used in this study (S. pullorum, S. typhimurium,S. typhi), but could not react with E. coli. The potential of cross-reaction of serum in S. enteritidis-vaccinatedchicken to some serotypes of S. enterica may play a role in reducing the infection caused by that serotype.
Edible Bird’s Nest as Potential Food with Anti-Viral and Anti-Inflammatory Properties Against Covid-19: an in Silico Study Siti Gusti Ningrum; Rochiman Sasmita; Viol Dhea Kharisma
Acta VETERINARIA Indonesiana Vol. 11 No. 1 (2023): Maret 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.1.43-50

Abstract

The Chinese believe consuming edible bird’s nests (EBN) can increase immunity to various diseases, including Covid-19. This study attempts to identify SARS COV-2-specific anti-viral and anti-inflammatory agents of EBN. We gathered samples from PubChem and Protein Data Bank (PDB). Afterwards, drug likeness was examined using the Lipinski model from the SCFBIO online service. The PASS web server analyzed the bioactive likelihood of chemicals found in EBN. Using PyRx 0.8 software with the blind docking technique. The PoseView web server and PyMol v2.4.1 software were utilized to ascertain molecular interactions. The in silico results show the potential of EBN as food therapy for Covid-19 sufferers, which is indicated by the presence of bioactive compounds from edible bird’s nest consisting of 9-O-acetylated GD3, glycopeptide, N-acetyl neuraminic acid, N-glycolyl-neuraminic acid, sialic acid, and tetra acetyl-thymol-beta-D-glucoside. These bio compounds are predicted to work as anti-viral and anti-inflammatory candidates against SARS-COV-2.
Prevalence of Endoparasite Infestation of Edible-Nest Swiftlet (Aerodramus fuciphagus) in Swiftlet Houses in Central Java, Indonesia Siti Gusti Ningrum
Acta VETERINARIA Indonesiana Vol. 12 No. 2 (2024): Juli 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.2.148-153

Abstract

Penelitian ini bertujuan untuk menentukan prevalensi infestasi endoparasit pada burung walet (Aerodramus fuciphagus) di Provinsi Jawa Tengah, Indonesia. Sebanyak 76 sampel feses segar burung walet (A. fuciphagus) dikoleksi dari beberapa rumah burung walet di Jawa Tengah. Metode deteksi endoparasit dalam penelitian ini menggunakan kombinasi metode natif, metode sedimentasi, dan metode apung. Berdasarkan hasil penelitian ini, prevalensi infestasi endoparasit pada burung walet (A. fuciphagus) di Jawa Tengah adalah 28%. Endoparasit yang ditemukan dalam penelitian ini antara lain Tetrameres spp. (7%), Notocotylus sp. (11%), dan Raillietina sp. (11%). Penelitian ini berhasil memberikan data kejadian infestasi endoparasit di burung walet (A. fuciphagus).
Host Range Bakteriofag Yang Diisolasi Dari Limbah Di Lingkungan Rumah Burung Walet Dewantoro, Bagas Satrio Dewantoro; Siti Gusti Ningrum
Prosiding Seminar Nasional Kusuma Vol 2 (2024): Prosiding Seminar Nasional Kusuma
Publisher : LPPM UWKS

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Abstract

Latar belakang Bakteriofag adalah salah satu organisme hidup mikroskopis paling umum yang menghuni bumi ini. Bakteriofag merupakan virus yang dapat menginfeksi bakteri. Bakteriofag merupakan virus yang memiliki sifat parasit obigat terhadap bakteri. Tujuan dari penelitian ini adalah untuk mengetahui status narrow atau broad host range dari bakteriofag yang diisolasi dari limbah di lingkungan rumah burung walet. Kultur bakteri Stenotrophomonas maltophilia, Pseudomonas putida dan Priestia megaterium digunakan dalam uji host range metode spot test kemudian dilanjutkan dengan metode plaque assay untuk mengetahui jumlah titer bakteriofag. Media yang digunakan adalah Tryptone Soya Agar, Tryptone Soya Agar semi solid, dan Brain Heart Infusion Broth. Kultur bakteri Stenotrophomonas maltophilia, Pseudomonas putida dan Priestia megaterium pada media Tryptone Soya Agar dan diinkubasi selama 24 jam dalam suhu 30˚C. Bakteri diambil koloni terpisah dengan ose bulat lalu dicelupkan pada erlen mayer yang berisi Brain Heart Infusion Broth dan diinkubasi sampai terlihat keruh pada inkubator shaker dengan suhu 30˚C. Jenis penelitian adalah penelitian deskriptif observasional. Hasil penelitian yang menunjukkan bahwa bakteriofag mampu melisiskan bakteri Stenotrophomonas maltophilia dikonfirmasi terbentuknya plak clear atau zona bening metode spot test pada cawan petri. Pada percobaan uji host range dengan menggunakan tiga bakteri Stenotrophomonas maltophilia, Pseudomonas putida dan Priestia megaterium didapatkan hasil bahwa bakteriofag hanya dapat menginfeksi dan melisiskan bakteri Stenotrophomonas maltophilia. Dapat disimpulkan bahwa host range bakteriofag yang diisolasi dari limbah di lingkungan rumah burung walet adalah memiliki kisaran inang Narrow atau sempit. Kesimpulan Berdasarkan hasil penelitian yang dilakukan, kesimpulannya bahwa host range atau kisaran inang bakteriofag dari limbah dilingkungan rumah burung walet adalah narrow atau sempit. Karena hanya mampu melisiskan satu bakteri Stenotrophomonas maltophilia dikonfirmasi munculnya clear plak pada uji spot test.
Co-Authors Denny Widaya Lukman Dewantoro, Bagas Satrio Dewantoro Erfiandini Eka Puspita Fachriyan Hasmi Pasaribu I wayan Teguh Wibawan Rochiman Sasmita Viol Dhea Kharisma Wyanda Arnafia