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All Journal E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)
I MADE SUDARMA
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry

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Identifikasi Bakteri Penyebab Penyakit Utama pada Tanaman Hidroponik DEWA AYU DIAH PUSPITASARI; I PUTU SUDIARTA; I MADE SUDARMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Identification of Bacteria Causing the Main Diseases in Hydroponic Plants Hydroponics is a horticultural cultivation technique using water as its growing medium. As the times progressed, people began to switch to planting with a hydroponic system, but this system was not completely free from pathogen attacks, one of which was bacteria. Therefore, this study aims to identify the types of bacteria that can infect hydroponic plants and determine the percentage of disease in hydroponic plants. The research implementation includes (1) calculating the percentage of disease, (2) sampling, (3) symptomatic plant isolation, (4) inoculation, (5) morphological classification, (6) detection of bacteria by PCR (Polymerase Chain Reaction), and sequencing analysis. The results showed that the percentage of attack on three plants, namely lettuce with soft rot symptoms, was 31.6% and celery with bacterial blight symptoms was 56.1%. The highest attack percentage was on tomato plants with bacterial wilt symptoms, reaching 69.1%. PCR results showed DNA bands measuring ± 1500 bp using general 16S rRNA primers (27F forward and 1492R reverse) on the three plants. Sequence analysis was continued on bacterial isolates from tomato plants because they had the highest percentage of attack. The nucleotide analysis of bacterial isolates in tomato plants indicated that the bacteria were a genus of Pseudomonas sp. Homology level of Pseudomonas sp. from Kesiman Village, East Denpasar District, Bali, after being traced and matched to the GenBank data in the NCBI program, it has similar homology with Pseudomonas from Iraq and India with a homology level of 89.86%, as well as from Egypt, New Zealand, and Germany with a sequence homology level of 89.19%.
Potensi Jamur Asal Rizosfer Tanaman Cabai Rawit (Capsicum frutescens l.) Sehat dari Desa Bumbungan Kecamatan Banjarangkan Kabupaten Klungkung dalam Upaya Mengendalikan Penyakit Layu Fusarium secara In Vitro FERDIANSYAH DWI MAULANA; I MADE SUDARMA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The potential of rhizosphere fungi from health cayenne pepper plant (Capsicum frutescens L.) from Bumbungan Village to control the wilt disease Fusarium in vitroThe objectives of the research were to find out the potential and level domination (prevalance) of rhizosphere fungi from health cayenne pepper plant (Capsicum frutescens L.) to control the wilt disease Fusarium. It was done by using the in vitro inhibition test of rhizophere fungi againts the Fusarium oxysporum f.sp. capsici with dual culture technique. The study included 1) rhizosphere soil sampling of health cayenne pepper plants; 2) isolation and identification of soil fungi; 3) isolation of the pathogen Fusarium oxysporum f.sp capsici; 4) purification of pathogenic isolates of Fusarium oxysporum f.sp capsici; 5) prevalence of fungal isolates; 6) testing the inhibition of rhizosphere fungi on the growth of the fungus Fusarium oxysporum f.sp. capsici. The result showed that the identified rhizosphere fungi were the genus of Aspergillus spp., Penicillium spp., Trichoderma spp. The prevalence of fungi in the rhizosphere that dominate the healthy cayenne pepper plant is the genus of Penicillium spp. and Aspergillus spp. which have the same prevalence rate of each, 42.1 %. while the fungus Trichoderma spp. has prevalence rate 15,8 % . The results showed that all the rhizosphere fungi have potential to control wilt disease Fusarium oxysporum f.sp. capsici with different levels of inhibition. Based on the results of this study, further research needs to be done in order to apply directly the identified antagonist fungi to plant that already affected with wilt diseas
Co-Authors DEWA AYU DIAH PUSPITASARI FERDIANSYAH DWI MAULANA I Putu Sudiarta NI WAYAN SUNITI