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PENGUJIAN DAYA HAMBAT EKSTRAK BEBERAPA TUMBUHAN TERHADAP JAMUR CERATOCYSTIS SP. PENYEBAB PENYAKIT BUSUK BERAIR PADA BUAH SALAK BALI SUNITI, NI WAYAN
AGRITROP Vol. 26, No. 2 Juni 2007
Publisher : AGRITROP

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The research aimed to determine the inhibition activity of some plant extracts against Ceratocystis sp. on PDA medium, salacca piece, whole fruit and liquid medium. Research?s result showed that among nine plant extracts, two extracts inhibited the growth of Ceratocystis effectively, namely extract of Albinia galanga rhizome and boiled papaya leaf. Albinia galanga rhizome showed inhibitory 92.5 % on PDA medium and 89.6% on PD Broth liquid medium. Boiled papaya leaf extract showed 73.3% on PDA medium and 57.7 % on PD Broth liquid medium. On fruit piece and whole fruit inhibition activity of boiled papaya leaf extract has higher than to Albinia galanga rhizome extract.
Pengendalian Penyakit Layu Fusarium pada Tanaman Cabai Besar (Capsicum annuum L.) dengan Kompos dan Pupuk Kandang yang dikombinasikan dengan Trichoderma sp. di Rumah Kaca SUTARINI, NI LUH WAHYU; SUMIARTHA, I KETUT; SUNITI, NI WAYAN; SUDIARTA, I PUTU; WIRYA, G.N.ALIT SUSANTA; UTAMA, MADE SUPARTHA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Utilization of Trichoderma sp. combined with compost and manure to controlling Fusarium wilt disease on long chilli (Capsicum annuum L.) in greenhouse This study aims to determine the effectiveness of Trichoderma sp. combined with compost and manure to controlling Fusarium wilt disease in long chilli (Capsicum annuum L.). Laboratory studies conducted at the Laboratory of Plant Pathology Faculty of Agriculture, University of Udayana and field research conducted in the greenhouse in the village Pancasari Sukasada Buleleng Subdistrict. The experimental design used was completely randomized designs (CRD) with five treatments were repeated 5 times, each treatment consisted of 5 polybag. The treatments used in this study are Po: control (soil + F. oxysporum f.sp. capsici treatment); P1: compost + soil + F. oxysporum f.sp. capsici; P2: compost + Trichoderma sp. + soil + F. oxysporum f.sp. capsici; P3: cow manure + Trichoderma sp. +  soil  + F. oxysporum f.sp. capsici; and P4: chicken manure + Trichoderma sp. + soil  + F. oxysporum f.sp. capsici The results showed that treatment of Trichoderma sp. able to inhibit the growth of F. oxysporum f.sp. capsici with a percentage of 86.05% when compared to the control treatment at 7 days after inoculation observation in vitro. Application of Trichoderma sp. in compost and manure (cow manure and chicken) were able to suppress Fusarium wilt disease in the greenhouse with the lowest percentage of disease were in P3 and P4 treatment by 4.0% in the observation of 16 week after planting compared with 48.0% of control. Further, the application of Trichoderma sp. in compost and manure (cow manure and chicken) have yields greater than the control (soil without Trichoderma sp.) Keywords: Trichoderma sp., F. oxysporum f.sp. capsici, long chili (Capsicum annuum L.), compost, cow manure, chicken manure
UTILIZATION OF RHIZOSPHERE FUNGI TO CONTROL Fusarium oxysporum f.sp. capsici IN VITRO I Made Sudarma; Ni Made Puspawati; Ni Wayan Suniti; I Nyoman Wijaya; I Gusti Ngurah Bagus
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Fusarium caused wilt disease in chilli pepper and destroyed some farmer crops. Results ofpreliminary research has been discovered that the disease caused by Fusarium oxysporum f.sp. capsici.The alternative environmental friendly method is to find antagonist microbes which is located in therhizosphere of healthy pepper plants. This study aims to find out potentially antagonistic fungi tocontrol Fusarium wilt disease on pepper plants. The fungi were isolated by soil dilution method orviable plate count method on Potato Dextrose Agar medium with antibiotic Livoplaxacin (25%, w/v).Rhizosphere fungi from healthy pepper plants had been identified. A total of 63 spesies belong to 4genera included Penicillium (45 species), Aspergillus (6 species), Trichoderma (9 species) andCandida (3 species). The highest percentage of distribution of rhizosphere fungi are P. digitatum(47.63%), P. expansum (19,05%), T. harzianum (9,53%), A. nidulans, A. niger, Penicillium sp.,Candida albicans, and T. vitrens i.e 4,76% respectively. All of rhizosphere fungi colonies were foundto inhibit Fusarium oxysporum f.sp. capsici in vitro. The best inhibition was found in Aspergillusniger at 88.89 ± 2.2% followed by A. nidulans of 85,56 ± 1,6 %, T. harzianum at 84,45 ± 1,58% , andT. virens by 83,33 ± 1,2%, five days after inoculation. All of them have a very high inhibition criteria.
SOSIALISASI PENYAKIT LAYU PISANG DAN CARA MEMBUAT BIBIT DARI MATA TUNAS DI DESA LEMBONGAN, KECAMATAN NUSA PENIDA KABUPATEN KLUNGKUNG N. W. Suniti
Buletin Udayana Mengabdi Vol 12 No 1 (2013): Volume 12 No.1 – April 2013
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Training to control banana wild disease and to make the healthy banana seed with Persada biopesticide andBiota-L botanical pesticide was held at Lembongam village District of Nusa Penida, Klungkung regency on 28Juli 2012. Their activities were aimed to improve the farmer knowledge of banana wild disease and made thebanana healthy seed and control the banana wild disease. The topics covered were symptom of banana disease,bioecology pathogen, pathogen distribution, control of banana wild disease and made the banana healthy seedwith Persada biopesticide and Biota-L botanical pesticide. The training was attended by 31 participants from localfarmer of Lembongan village. The methods used in activities were lecture, trainning to make banana healthyseed, application of Persada biopesticide and Biota-l botanical pesticide in field. All farmers were interested inthis program because there was the new knowledge for them and they hope this to be continued in the future,
SUPPRESSION ABILITY OF CRUDE EXTRACT DERIVED FROM MARINE BIOTA AGAINST FUSARIUM OXYSPORUM F.SP. VANILLAE I Ketut Suada; Ni Wayan Suniti
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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The objective of this research was to investigate suppression ability of marine biota extracts against Fusarium oxysporum f.sp. vanillae of vanilla stem rot. Samples were collected at intertidal zones and in the depth of 1-7 m from seven beaches in Bali. Screening of active compounds of biota extracts were conducted using inhibition zone of well diffusion method on Potato Dextrose Agar (PDA). The extract was tested in-vitro in PDA medium using completely randomized design with three replicates. The methanolic extract of Aglaophenia sp. was able to suppress the growth of F. oxysporum f.sp. vanillae effectively, with minimum inhibition concentration (MIC) of 0.05 %. The extract inhibited colony growth diameter and total mycelial dry weight.
Pengaruh Penggunaan Beberapa Paket Teknologi terhadap Perkembangan Penyakit Layu Fusarium pada Tanaman Cabai Rawit (Capsicum frutescens L.) dan Cabai Besar (Capsicum annuum L.) di Dataran Tinggi I WAYAN RUSMAN; NI WAYAN SUNITI; I KETUT SUMIARTHA; I PUTU SUDIARTA; GUSTI NGURAH ALIT SUSANTA WIRYA; I MADE SUPARTHA UTAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Influence of Some Packages of Technology to Fusarium Wilt Disease Development on Cayenne Chili Plants (Capsicum frutescens L.) and Long Chili (Capsicum annuum L.) in the HighlandsThe research was conducted in order to determine the influence and effectiveness of some technologies of Trichoderma sp. compost, rain shelter and pesticides to control the development of fusarium wilt disease on long chili and cayenne chili plants in the highlands. This research was done from September 2017 to February 2018, taking place in Pancasari Village, Buleleng Regency and in the Laboratory of Plant Disease, Udayana University. The study used a Factorial Randomized Block Design with two factors and replicated four times. The first factor was chili type, long chili and cayenne chili. The second factor was the use of technologies, Trichoderma sp. compost, rain shelter and pesticide. The variables observed in this research were symptoms and percentage of fusarium wilt disease. The results showed that all treatment technologies effectively suppressed disease development when compared to control treatment. Percentages of damage by fusarium wilt disease on Trichoderma sp., rain shelter, pesticide and control treatment were 22.50%, 23.75%, 27.50% and 45.00%, respectively.
Epidemi Penyakit Antraknosa pada Tanaman Jeruk Nipis [Citrus aurantifolia (christm.) Swingle] di Desa Kertalangu, Kecamatan Denpasar Timur NI WAYAN SUNITI; I KETUT SUADA; I MADE SUDARMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.1, Januari 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Epidemic of Anthracnose Disease in the Lemon [Citrus aurantifolia (Christm.) Swingle] at Kertelangu Village, East Denpasar District           Anthracnose disease on lemon  was a newly discovered disease in Kertalangu Village, East Denpasar. Status of the disease unknown,. and lemon plants grown less well maintained and neglected, almost all plants showing symptoms of disease. Planting conditions like this it's possible anthracnose disease that can destroy rapidly growing cultivation of lemon. The research was conducted from April to June 2015 in the village of Kertalangu, district East Denpasar, Bali.           The results showed that the leaf spot disease found on lemon was anthracnose that caused by Colletotrichum gloeosporioides. Disease incidence of anthracnose was 26.16 – 35.00%, and disease severity was 17.38 – 24.10%, that is mean relatively mild disease. The infection of rate was  0.00128 – 0.0162 per leaf per day, its mean development of the disease was slowly.   Keywords: Anthracnose, Colletotrichum gloeosporioides, inhibiting ability, prevalence, endophytic and exophytic.  
Uji Daya Hambat Bakteri Paenibacillus polymyxa terhadap Pertumbuhan Jamur Colletotrichum sp. Secara In Vitro IDA AYU ISTRI MAYADIANTI; KHAMDAN KHALIMI; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Inhibition Test of Paenibacillus polymyxa on the Growth of the Fungus Colletotrichum sp. in vitro Fungus Colletotrichum sp. is an airborne pathogen that causes anthracnose disease in chili. Utilization of biological agents of Paenibacillus polymyxa is the choice in suppressing the growth and fungal attack of Colletotrichum sp. The use of biological agency is environmentally friendly. The purpose of this study was to determine the inhibitory properties of P. polymxya bacteria against the growth of the fungus Colletotrichum sp. in vitro.The results showed that P. polymyxa isolates C1 was able to inhibit the growth of C. acutatum isolates BLCB, C. gloesporioides isolates KLCR2, C. scovillei isolates SGCR, C. fructicola isolates KRCR, C. truncatum isolates DPCR2 and C. nymphae isolates BLCR, with the percentage of inhibition ranging from 82.74% to 86.52%. Bacterial filtrate P. polymyxa C1 was able to inhibit the growth of fungal colonies of C. acutatum isolate BLCB, C. gloesporioides isolate KLCR2, C. scovillei isolates SGCR , C. fructicola isolates KRCR, C. truncatum isolates DPCR2 and C. nymphaeae isolates BLCR with inhibitory power percentage ranging from 71.90% to 82.48%.
Uji Aktivitas Antijamur Bacillus cereus terhadap Colletotrichum fructicola KRCR Penyebab Penyakit Antraknosa pada Buah Cabai Rawit (Capsicum frutescens L.) KRISNA SANUBARI PURBA; KHAMDAN KHALIMI; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.1, Januari 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Test of Antifungal Activity of Bacillus cereus Against Colletotrichum fructicola Causing Anthracnose Disease in Cayenne Pepper (Capsicum frutescent L.) Anthracnose or fruit rot disease that attacks ceyenne pepper is caused by the pathogenic fungus Colletotrichum fructicola, this disease can cause crop failure. Currently, control of this disease is still using synthetic fungicides, but this method can cause demage to the ecosystem. Biopesticides are one of the enviromentally friendly control of plant phatogens because biopesticides use biologycal agents. The purpose of this study was to determine the ability of B. cereus bacteria to inhibit the growth of C. fructicola KRCR cause anthracnose desease in vitro. The result showed that bacteria B. cereus was able to inhibit the growth of fungal colonies C. fructicola KRCR on potato dextrose agar (PDA) with an inhibitory percentage of 90.55% when compared to controls. The results of the B. cereus filtrate test a concentration of 50% is able to inhibit the growth of C. fructicola KRCR with an inhibitory percentage of 87.56%.
Pengendalian Penyakit Layu Fusarium oxysporum f.sp. capsici pada Tanaman Cabai Rawit Capsicum frutescens di Rumah Kaca dengan Trichoderma sp yang Ditambahkan pada Kompos I MADE TEDI MAHADI PUTRA; TRISNA AGUNG PHABIOLA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.1, Januari 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The utilize of Trichoderma sp Compost Formulation to Control wilt disease of Fusarium oxysporum f.sp.capsici in cayenne pepper Fusarium oxysporum f.sp.capsici is a disease that attacks cayenne pepper. This disease can be suppressed by an infectious pathogen called Trichoderma sp. This study was done at laboratory UPT BPTPH Bali Province started from April 2018 to September 2018. This study was done in order to fine the best dose of Trichoderma sp. that used to control Fusarium oxysporum f.sp.capsici on cayenne pepper The experimental design is randomized block design (RBD) with six treatments and each treatment is replicated 4 times. The result of this study show that application of Tricoderma sp. on cayenne pepper could decrease wilt disease of Fusarium oxysporum f.sp. capsici with minimum percentage of wilt symptoms is T5 only 30% in 16 WAT compare with control 67,5%. The best treatment that can decrease symptoms of wilt desease and increasing the yield of cayenne pepper is the T5 (25 gr/plant) but not significantly affect with T3 (12 gr/plant).