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Vanda S Kamu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Mathematics Physics

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SINTESIS KOMPOSIT KITOSAN/MgO/Ag DAN ANALISIS EFEKTIVITASNYA SEBAGAI ANTIBAKTERI Maryani, Shintya; Aritonang, Henry F; Kamu, Vanda S
CHEMISTRY PROGRESS Vol 14, No 1 (2021)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.14.1.2021.34291

Abstract

Penelitian tentang sintesis komposit kitosan/MgO/Ag dengan variasi massa prekursor AgNO3 0,05 g, 0,1 g, 0,15 g dan 0,2 g disintesis dengan metode kopresipitasi. Komposit yang dihasilkan dikarakterisasi menggunakan X-Ray Diffractometry (XRD) dan Scanning Electron Microscope (SEM). Selanjutnya, keefektifan komposit ini sebagai antibakteri diuji yaitu Staphylococcus aureus (Gram positif) dan Escherichia coli (Gram negatif). Hasil penelitian menunjukkan bahwa puncak karakteristik partikel MgO dan Ag tidak muncul pada difraktogram XRD, tetapi secara visual dan morfologis SEM mendukung pembentukan dua partikel dan berdasarkan literatur dari hasil peneliti lain. Selain itu, komposit ini efektif dalam menghambat pertumbuhan kedua bakteri.       ABSTRACT Research on the synthesis of chitosan/MgO/Ag composites with variations in the mass of AgNO3 precursors 0.05 g, 0.1 g, 0.15 g and 0.2 g were synthesized by the coprecipitation method. The resulting composites were characterized using X-Ray Diffractometry (XRD) and Scanning Electron Microscope (SEM). Furthermore, the effectiveness of this composite as an antibacterial was tested, namely Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). The results showed that the characteristic peaks of MgO and Ag particles did not appear on the XRD diffractogram, but visually and morphologically SEM supported the formation of two particles and based on the literature from the results of other researchers. In addition, this composite is effective in inhibiting the growth of both bacteria.
AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DAN FRAKSI HEKSANA DAUN SOYOGIK (Saurauia bracteosa DC) TERHADAP OKSIDASI ASAM LINOLEAT Runtuwene, Max R. J.; Kamu, Vanda S; Rotty, Marsella
CHEMISTRY PROGRESS Vol 14, No 2 (2021)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.14.2.2021.37559

Abstract

Soyogik (Saurauia bracteosa DC) merupakan tanaman obat yang berasal dari Minahasa Tenggara, Provinsi Sulawesi Utara. Penelitian ini bertujuan untuk menentukan potensi aktivitas antioksidan ekstrak dan fraksi pelarut dari daun soyogik. Ekstraksi dilakukan dengan maserasi menggunakan metanol dan fraksinasi dengan n-heksana dan etil asetat. Kandungan total fenolik ekstrak dan fraksi diukur menggunakan uji Folin-Ciocalteu sedangkan aktivitas antioksidan dievaluasi dengan penghambatan oksidasi asam linoleat dengan metode Ferric Thiocyanate (FTC) dan penghambatan malonaldehid (MDA) dengan metode Thiobarbituric Acid Reactive Substance (TBARS). Hasil penelitian menunjukkan bahwa kandungan fenolik yang dinyatakan dalam ekuivalen asam galat dari ekstrak dan fraksi berkisar antara 50,83-99,67 g/mL. Fraksi etil asetat memiliki kandungan fenolik yang lebih tinggi dibandingkan fraksi heksana dan ekstrak metanol. Hasil penelitian ini juga menunjukkan bahwa fraksi etil asetat, n-heksana, dan α-tokoferol memiliki penghambatan peroksida masing-masing sebesar 50,21, 44,26, dan 46,96% dan penghambatan malonaldehid masing-masing 91,03, 88,81, dan 89,48%. Kemampuan hambat peroksida dan malonaldehid menunjukkan bahwa fraksi etil asetat memiliki aktivitas antioksidan yang lebih tinggi dibandingkan fraksi n-heksana dan a-tokoferol sebagai kontrol positif.ABSTRACT Soyogik (Saurauia bracteosa DC) is a medicinal plant originating from Southeast Minahasa, North Sulawesi Province. The objective of study was to determine the potential antioxidant activity of the extract and the solvent fraction of soyogik leaves. Extraction was carried out by maceration using methanol and fractionation with n-hexane and ethyl acetate. The total phenolic contents of extract and fractions were measured using a Folin-Ciocalteu assay while the antioxidant activities was evaluated by the inhibition of linoleic acid oxidation with the Ferric Thiocyanate (FTC) and measuring the malonaldehyde (MDA) inhibition with the Thiobarbituric Acid Reactive Substance (TBARS) methods. The results showed that phenolic content expressed in gallat acid equivalent of extract and fractions ranged from 50.83-99.67 µg/mL. The ethyl acetate fraction has higher phenolic content than hexane fraction and methanol extract. The results showed also that the ethyl acetate, n-hexane fractions and a-tocopherol had peroxide inhibition were 50.21, 44.26, and 46.96%, respectively and the inhibition of malonaldehyde were 91.03, 88.81, and 89.48% respectively. The inhibitory ability of peroxides and malonaldehyde showed that the ethyl acetate fraction had higher antioxidant activity than the n-hexane and a-tocopherol fractions as positive controls.
Isolasi dan Karakterisasi Pati dari Kulit Singkong (Manihot esculenta) sebagai Bioflokulan untuk Menurunkan Kadar Limbah Fenol dan p-Klorofenol Pairunan, Regina; Suryanto, Edi; Vanda S Kamu
CHEMISTRY PROGRESS Vol. 19 No. 1 (2026)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.19.1.2026.68062

Abstract

ABSTRAK Penelitian ini menguji efektivitas pati kulit singkong sebagai bioflokulan organik dalam pengolahan limbah kimia fenol dan p-klorofenol. Fokus utama studi adalah menganalisis pengaruh proses gelatinisasi, variasi dosis pati, serta durasi pengadukan terhadap efisiensi penurunan kadar polutan. Proses dimulai dengan isolasi pati lewat deproteinasi menggunakan larutan NaOH 0,5 M. Selanjutnya, flokulasi diuji pada 10 ml sampel limbah dengan memvariasikan dosis bioflokulan (0,2; 0,5; dan 1 gram) serta waktu pengadukan (15, 30, dan 60 menit). Hasil eksperimen membuktikan bahwa pati kulit singkong sangat efektif sebagai agen pengolah limbah. Kondisi operasional optimum tercapai pada dosis 0,5 gram dengan waktu pengadukan 30 menit, yang mampu mereduksi kadar fenol sebesar 85,32% dan p-klorofenol sebesar 84,32%. ABSTRACT This study evaluates the effectiveness of cassava peel starch as an organic bioflocculant for treating phenol and p-chlorophenol chemical wastewater. The primary focus of the study is to analyze the effects of the gelatinization process, variations in starch dosage, and stirring duration on pollutant reduction efficiency. The process began with starch isolation via deproteinization using a 0.5 M NaOH solution. Subsequently, flocculation was tested on 10 mL wastewater samples by varying the bioflocculant dosages (0.2, 0.5, and 1 g) and stirring times (15, 30, and 60 min). Experimental results demonstrate that cassava peel starch is highly effective as a wastewater treatment agent. The optimum operational conditions were achieved at a dosage of 0.5 g with a stirring time of 30 min, which reduced phenol and p-chlorophenol levels by 85.32% and 84.32%, respectively.
Co-Authors Andre G Kalensun Audy Denny Wuntu Ba'u, Defrikson Dewa Gede Katja Edi Suryanto Henry Fonda Aritonang Jemmy Abidjulu Jemmy Abidjulu Maryani, Shintya Maureen Kumaunang Maureen Kumaunang Max R. J. Runtuwene Max R.J Runtuwene Pairunan, Regina Paulina yamlean Rotty, Marsella