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Max R.J Runtuwene
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Mathematics Physics

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PENENTUAN TOTAL FENOLIK DAN UJI AKTIVITAS ANTIOKSIDAN PADA BIJI DAN KULIT BUAH PINANG YAKI (Areca vestiaria Giseke) Jefriyanto Ismail; Max R.J Runtuwene; Feti Fatimah
JURNAL ILMIAH SAINS Volume 12 Nomor 2, Oktober 2012
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.248 KB) | DOI: 10.35799/jis.12.2.2012.557

Abstract

PENENTUAN TOTAL FENOLIK DAN UJI AKTIVITAS ANTIOKSIDAN PADA BIJI DAN KULIT BUAH PINANG YAKI (Areca vestiaria Giseke)ABSTRAK Telah dilakukan penelitian untuk menentukan kandungan total fenolik dan menguji aktivitas antioksidan pada biji dan kulit buah pinang yaki. Ekstrak biji dan kulit buah pinang yaki diperoleh dengan maserasi tanpa sokletasi dan maserasi setelah sokletasi. Total fenolik diuji dengan metode Folin-Ciocalteu dan uji aktivitas antioksidan dengan metode DPPH. Hasil penelitian yang diperoleh, kandungan total fenolik Maserasi Biji Setelah Sokletasi, Maserasi Kulit Setelah Sokletasi berturut-turut memiliki nilai sebagai berikut : 85,92 mg/kg, 3,16 mg/kg. sedangkan aktivitas antioksidan Maserasi Biji Setelah Sokletasi, Maserasi Kulit Setelah Sokletasi berturut-turut memiliki nilai sebagai berikut : 88,16%, 54,11%. Kata kunci : Antioksidan, Areca vestiaria Giseke, DPPH, Total Fenolik   TOTAL PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF THE SEED AND SKIN OF PINANG YAKI (Areca vestiaria Giseke) FRUITS ABSTRACT This research has been conducted to measure the total phenolic compounds and the antioxidant activity of the seed and skin of pinang yaki fruits. The samples of the seed and skin were prepared separetely by maceration and soxhlet extraction followed by maceration. Folin-Ciocalteu method was used to measure the total phenolic compounds of while DPPH (1,1-diphenyl-2-pikrilhidrazil) method was used to measure the antioxidant activity. The total phenolic compounds of the seed by soxhlet extraction followed by maceration, skin by soxhlet extraction followed by maceration and were 85,92 mg/kg, 3,16 mg/kg respectively. The antioxidant activity of the seed by soxhlet extraction followed by maceration, skin by soxhlet extraction followed by maceration and were 88,16%, 54,11% respectively. Keywords : Antioksidant, Areca vestiaria Giseke, DPPH, Total Phenolic Compounds
ANALISIS KANDUNGAN PATI DALAM BATANG TANAMAN AREN (Arenga pinnata) Jardewig E Manatar; Julius Pontoh; Max R.J Runtuwene
JURNAL ILMIAH SAINS Volume 12 Nomor 2, Oktober 2012
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.761 KB) | DOI: 10.35799/jis.12.2.2012.560

Abstract

ANALISIS KANDUNGAN PATI DALAM BATANG TANAMAN AREN (Arenga pinnata) ABSTRAK Penelitian dilakukan untuk menganalisis kandungan pati dalam batang tanaman aren (Arenga pinnata) dengan caramengetahui variabilitas kandungan pati pada pohon aren di beberapa lokasi yang berbeda di Tomohon dan hubungannya dengan beberapa faktor produktivitas tanaman. Penelitian yang dilaksanakan meliputi pengambilan sampel, pengukuran kadar air, preparasi sampel, pembuatan kurva standar, dan analisis pati.  Hasilnya menunjukkan adanya perbedaan kandungan pati pada berbagai posisi di pengaruhi oleh produktivitas dari masing-masing pohon aren dari beberapa lokasi yang berbeda di Tomohon. Kandungan pati yang tertinggi diperoleh sekitar 1 m di bawah bunga betina pertama pada Posisi 5  bervariasi dari 26-37 %. Kandungan pati diatas 35 % dijadikan indikasi tanaman akan menghasilkan nira yang baik. Kata kunci : A. pinnata, Kandungan Pat.   ANALYSIS OF STARCH CONTENT IN STALK PALM SUGAR PLANT (Arenga pinnata) ABSTRACT The research do to analyze starch content in stalk palm sugar plant (Arenga pinnata) with to know the variability of starch content in palm sugar plant at some different location in Tomohon and the relationship with some productivity factors of plant. The research activities included starch sampling, measurement of water content, sample preparation, making standard curve and analysis of starch content. The results showed different starch content at various position at a tree and in the trees at various locations in Tomohon.  The starch content corelated to the productivity of each palm sugar trees at different location in Tomohon. The highest of starch content found about one meter under first female flower at the fifth position has variation from 26-37%. Starch content above 35% indicated that tree will produce juice. Keywords : A. pinnata, Starch Content
Analisis Fitokimia dan Uji Toksisitas Daun Leleng Merah (Graptophyllum pictum (L.) Griffith) dengan Metode Brine Shrimp Lethality Test Defrikson Ba'u; Dewa G Katja; Vanda S Kamu; Paulina V.Y Yamlean; Max R.J Runtuwene
JURNAL ILMIAH SAINS Volume 20 Nomor 2, Oktober 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.329 KB) | DOI: 10.35799/jis.20.2.2020.30069

Abstract

Penelitian ini bertujuan untuk menentukan total phenolic content (TPC), total flavonoid content (TFC), condensed tannin content (CTC) dan nilai toksisitas dari daun leleng merah. Daun leleng merah diekstraksi dengan metode maserasi dan dipartisi menggunakan pelarut n-heksana, etil asetat, dan air. Penentuan nilai TPC menggunakan metode Folin-Ciocalteu. Penentuan nilai TFC menggunakan metode AlCl3. Penentuan nilai CTC menggunakan metode vanillin-HCl. Penentuan nilai toksisitas menggunakan metode BSLT. Nilai TPC yang diperoleh pada fraksi n-heksana (FH), fraksi air (FA), dan fraksi etil asetat (FEA) daun Leleng Merah berturut-turut, adalah: 20,48; 31,7; dan 37,57 (mg/g). Nilai TFC diperoleh pada FH, FA, dan FEA berturut-turut, adalah: 0,64; 0,99; dan 1,27 (mg/g). Nilai CTC diperoleh pada FH, FA, dan FEA berturut-turut, adalah: 0,56; 2,17; dan 6,52 (mg/g). Nilai toksisitas diperoleh pada FEA, FA, FH berturut-turut, adalah: 21,93; 100,7; dan 117,22 (mg/L). FEA memiliki nilai toksisitas dan kandungan fitokimia tertinggi. Daun leleng merah bersifat toksik.Kata Kunci : Brine shrimp lethality test, leleng merah, fitokimia, Phytochemical Analysis and Toxicity Test of Leleng Merah Leaves (Graptophyllum pictum (L.) Griffith) Using the Brine Shrimp Lethality Test ABSTRACTThis study aims to determine the total phenolic content (TPC), flavonoids (TFC), condensed tannins (CTC) and toxicity value of Leleng Merah leaves. Leleng merah leaves were extracted by maceration method and partitioned using n-hexane, ethyl acetate, and water solvent. TPC values were determined using the Folin-Ciocalteu method. TFC values were determined using the AlCl3 method. CTC values were determined using the vanillin-HCl method. Toxicity values were determined using the BSLT method. TPC values obtained in the n-hexane (FH), water (FA), and ethyl acetate (FEA) fraction of the Leleng Merah leaves, were: 20.48; 31.7; 37.57 (mg / g) respectively. TFC values obtained in FH, FA, and FEA, were : 0.64; 0.99; 1.27 (mg / g) respectively. CTC values obtained at FH, FA, FEA, were: 0.56; 2.17;  6.52 (mg/g) respectively. Toxicity values obtained at FEA, FA, FH, were: 21.93; 100.7; 117.22 (mg/L) respectively. FEA has the highest toxicity and phytochemical content. Leleng Merah Leaf is toxic.Keywords : Brine Shrimp Lethality Test, leleng merah, phytochemistry
Co-Authors Ba'u, Defrikson Defrikson Ba'u Dewa G Katja Dewa Gede Katja Feti Fatimah Hilmayanti, Erina I Nyoman Adi Putra Jardewig E Manatar Jefriyanto Ismail Julius Pontoh Julius Pontoh Mantiri, Sisilia A Paulina yamlean Unang Supratman Vanda S Kamu Vanda S Kamu