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All Journal JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Akademika Biologi Media Farmasi Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Hermin Pancasakti Kusumaningrum
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Other

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Pertumbuhan Mikroalga Botryococcus braunii Sebagai Penghasil Lipid Pada Medium Campuran Antara Air Kelapa Dan Air Laut Bintoro Rudi Saputro; Endang Kusdiyantini; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions  for bioenergy.  Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be subtituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of  P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using haemocytometer. The results showed that 2.5% coconut water subtitution exhibited the best growth rate and lipid production, ie the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control. Keywords: Growth, Botryococcus braunii, Lipid, Coconut water, Seawater.
KERAGAMAN GENETIK JAHE (Zingiber officinale Roscoe) MENGGUNAKAN TEKNIK PENANDA MOLEKULER Random Amplified Polymorphic DNA (RAPD) Choirul Rizka Putri; Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Ginger plant in Indonesia is known there are three kinds of varieties, namely red ginger, emprit ginger and big ginger. Ginger (Zingiber officinale Roscoe) is one of the medicinal plants that could potentially be produced in increasing quantities and wide distribution in Indonesia that may affect patterns of genetic diversity. This study aims to determine patterns of genetic diversity of three varieties of ginger (red ginger, big ginger and emprit ginger) obtained from BALITRO, Bogor and BPTP, Ungaran using two primers (OPA1 and OPA2). Techniques of molecular markers RAPD (Random Amplified Polymorphic DNA) is one of the techniques used for the analysis of genetic diversity. The study begins by isolating DNA from 30 samples of ginger leaves, then amplification with primers OPA1 and OPA2 which then results of electrophoresis converted into the form of binary data and phylogenetic tree construction with 2:02 NYTSYS program. The results obtained are worth a total of 20 polymorphic band (86.9%) and 3 pita monomorphic (13%) with primary OPA1 and OPA2. Heterozygosity values in a row is worth 0.9026 and 0.889. Distance genetic similarities in this sample has the highest value is 1 and the lowest is 0.3. The relationship between the three varieties of big ginger are distant relationship with red ginger and emprit ginger. Red ginger and emprit ginger still have a close genetic relationship. Key Word               : Genetic Diversity, Zingiber officinale, RAPD
KOMUNITAS BAKTERI RHIZOSFER TEH MELALUI APLIKASI BIOIMUNIZER (Chryseobacterium sp. dan Alcaligenes sp.) DENGAN METODE TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) Elin Savitri Aviani; Dwi Ningsih Susilowati; Siti Nur Jannah; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Types of bacteria from the rhizosphere communities actually have been developed as bioimunizer but their consistency is still need further experiment about indigenous bacteria associated with rhizosphere of plants. This study aims to identification on the tea plant rhizosphere bacterial communities which in previous studies has been infected with E. vexans Massee and given bioimunizer as well as the checking existence Chryseobacterium sp. and Alcaligenes sp. The result research showed that communities of bacteria on control samples obtained through culturing Bacillus sp. (51.91%), Acidobacteria bacterium (39.42%) and Actinobacteria sp. (8.66%). Control sample through metagenom obtained Gemmatimonas aurantiaca (5.80%), Bacillus sp. (42.55%), Acidobacteria bacterium (23.45%) and Actinobacteria sp. (28.20%). Communities of bacteria in the samples treated by culturing obtained Gemmatimonas aurantiaca (3.58%), Bacillus sp. (30.76%), Pseudomonas sp. (5.55%) Acidobacteria bacterium (13.94%) and Actinobacteria sp. (46.16%). Communities of bacteria in the samples treated by metagenom found Bacillus sp. (10.66%), Acidobacteria bacterium (4.22%), Actinobacteria sp. (5.48%), Uncultured bacterium (1.49%), Alcaligenes sp. (36.95%) and Chryseobacterium sp. (46.82%). The existence of Alcaligenes sp. and Chryseobacterium sp. show consistency bioimunizer composition is applied. Communities of bacteria on metagenom have diversity and evenness level higher than the culturing approach. Keywords: bacterial communities, T-RFLP, Chryseobacterium sp., Alcaligenes sp.
KARAKTERISASI MORFOLOGI SEL REKOMBINAN DAN PIGMEN HASIL FUSI PROTOPLAS INTERSPESIFIK Phaffia rhodozyma DAN Chlorella pyrenoidosaH Chick Indah Sulistyarini; Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini
Media Farmasi Indonesia Vol. 8 No. 2 (2013): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

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Abstract

Protoplast fusion technique is a process offusion between protoplasts of theorganism to other organisms, so that the resulting recombinant cells with varying character derived from its parent. This study did Phaffia rhodozyma interspesies fusion and Chlorella pyrenoidosa. The purpose of this study was to analyze the morphological characteristics of cells results protoplast fusion recombinant yeast P. rhodozyma and microalgae C. pyrenoidosa. Protoplast fusion technique performed in 4 phases, namely the isolation of protoplasts, protoplast fusion, and analysis of recombinant cell morphological characters. The results showed that the recombinant cells have morphological characters derived from the parent P. rhodozyma and of the parent C. pyrenoidosa. But was character of the parent P. rhodozyma more dominant. Recombinant cells capable of producing pigment and lutein astaksanthin. Astaksanthin pigment levels in recombinant cell height in a shorter time than the parent P. rodozyma.
MOLECULAR IDENTIFICATION AND MORPHOLOGICAL CHARACTERIZATION OF PATCHOULI (Pogostemon sp.) FROM BATANG REGENCY, CENTRAL JAVA PROVINCE Hermin Pancasakti Kusumaningrum
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Patchouli (Pogostemon cablin Benth.) is an essential oil-producing plant from Batang Regency that has excellence in patchouli alcohol contents and highly survives in any condition. Molecular identification has been done in the ITS region because DNA sequences in the ITS rRNA region evolved faster than in other areas. This study aimed to get the molecular and morphological identity of patchouli (Pogostemon sp.) from BPP Kabupaten Batang. The study consisted of sample preparation, DNA isolation, amplification, electrophoresis, sequence data analysis, and phylogenetic analysis using MEGA X. All parts of plant were morphologically identified and compared with patchouli from Sidikalang, Aceh, Java, and China. Extraction DNA produced 301.7 ng ?L–1 concentration and 1.93 purity. Amplification of ITS fragment patchouli produced a 670 bp-sized single band. Phylogenetic analysis showed patchouli BPP related to Pogostemon cablin (KR608752.1) with 98% coverage identity. BPP patchouli showed 62,5% morphological similarity with Sidikalang patchouli compared to Java patchouli. In conclusion, BPP patchouli is a Sidakalang patchouli P. cablin that has undergone environmental adaptation.
Co-Authors Agnistisya Widaranti Anto Budiharjo Bintoro Rudi Saputro Choirul Rizka Putri Dwi Kristyaningrum DWI NINGSIH SUSILOWATI Elin Savitri Aviani Endang Kusdiyantini Endang Pancasakti Kusdiyantini Firly Putri Fardilla Hadi Ajie Endrawati Hadi Endrawati Indah Sulistyarini JOEDORO SOEDARSONO M Masfuroh Muhammad Ajie Zaenuri Muhammad Zaenuri Siti Nur Jannah Teuku Tajuddin Triwibowo Yuwono w wijanarka Yohanes Chandrawijaya Yuliandini Pangestika