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All Journal Jurnal Ilmu Pertanian JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Ganendra: Majalah IPTEK Nuklir Microbiology Indonesia Journal of Tropical Soils Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology
Triwibowo Yuwono
Laboratory Of Microbiology, Department Of Agricultural Microbiology, Faculty Of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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Ethanol Production by Fermentation of Various Sweet-Stalk Sorghum Juices Using Various Yeast Strains Widianto, Donny; Arofatullah, Akbar; Yuwono, Triwibowo; Prijambada, Irfan Dwidya
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.188 KB)

Abstract

The ethanol production by fermentation of sweet-stalk sorghum juice is affected by the juice composition and the capability of the yeast strain to ferment it. Eight yeast strains were tested on their growth and ethanol fermentation abilities in sweet-stalk sorghum juices extracted from three cultivars of sweet sorghum. The best specific growth rate of the yeast strains grown aerobically in the yeast extract peptone dextrose (YEPD) broth and the sweet-stalk sorghum juices of KCS105, FS501, and FS902 cultivars, were achieved by OUT7903, OUT7913, OUT7903, and OUT7027 yeast strains, respectively. However, the best specific CO2 evolution rate of the yeast strain during fermentation of the juices was achieved by OUT7027 yeast strains. The highest ethanol concentration, ethanol yield, and sugar conversion efficiency (SCE) were obtained by strain OUT7921 when it was employed to ferment sweet-stem sorghum juice of FS902 cultivar. It was also observed that the juice extracted from sweet-stalk sorghum of FS902 cultivar is the most suitable medium for all yeast strains to achieve their best fermentation abilities. Thus, it is likely that the growth and ethanol production ability of a yeast strain in sweet-stalk sorghum juice depend on the physiological responses of the yeasts to nutrientcomposition of the sorghum juice and the sorghum cultivar from which the juice was extracted.Key words : Sweet-stalk sorghum juice, ethanol, fermentation, yeast
Efek Pelaparan dan Akumulasi Polifosfat terhadap Biopresipitasi Uranium pada Bacillus cereus A66 Octavia, Bernadetta; Yuwono, Triwibowo; Taftazani, Agus
Biotropika: Journal of Tropical Biology Vol 6, No 2 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (972.828 KB) | DOI: 10.21776/ub.biotropika.2018.006.02.05

Abstract

Pelaparan dan akumulasi polifosfat pada bakteri diduga dapat meningkatkan biopresipitasi uranium. Tujuan penelitian ini adalah untuk mengetahui efek pelaparan dan akumulasi polifosfat terhadap peningkatan biopresipitasi uranium pada Bacillus cereus A66. Pada penelitian ini B. cereus A66 ditumbuhkan terlebih dahulu (prakultur) dalam Tryptone Glucose Yeast Extract (TGY), pada suhu ruang (± 28ºC) hingga fase logaritmik (± 16 jam). Pada perlakuan pertama, prakultur B. cereus A66 diberi perlakuan pelaparan fosfat dalam medium P-free, selanjutnya dipindahkan ke medium P-uptake untuk akumulasi fosfat. Untuk mengamati biopresipitasi uranium, sel bakteri dipindahkan ke dalam larutan uranium 1 mM. Pada perlakuan kedua, prakultur B. cereus A66 langsung dipindahkan ke dalam larutan uranium tanpa fase pelaparan dan akumulasi polifosfat. Sedangkan pada perlakuan ketiga,  B. cereus A66 tanpa fase pelaparan dikultur dalam medium P-uptake kemudian dipindahkan ke larutan uranium. Pada perlakuan keempat, B. cereus A66 dikondisikan dengan pelaparan fosfat dalam medium P-free, diikuti dengan pemindahan ke dalam larutan uranium. Perlakuan kedua, ketiga dan keempat dirancang untuk mengkonfirmasi efek perlakuan pertama dalam penelitian ini. Hasil penelitian menunjukkan bahwa B. cereus A66 yang mengalami pelaparan fosfat dapat mengakumulasi fosfat delapan kali lebih banyak ketika dipindahkan ke medium P-uptake, dibandingkan dengan B. cereus A66 yang tidak mengalami pelaparan fosfat. Selain itu, B. cereus A66 yang mengakumulasi lebih banyak fosfat juga menunjukkan peningkatan biopresipitasi uranium sebesar 1,5 kali lebih banyak dibandingkan dengan B. cereus A66 yang tidak mengalami pelaparan fosfat. Fenomena ini diyakini digerakkan oleh metabolisme polifosfat yang dikontrol oleh aktivitas gen PPK dan PPX. Secara keseluruhan hasil penelitian ini menunjukkan bahwa semakin banyak polifosfat terakumulasi dalam sel, semakin meningkat respon biopresipitasi uranium dalam hal jumlah uranium yang diambil dari larutan dan efisiensi waktu pengambilannya. Strategi ini dapat dikembangkan lebih lanjut untuk bioremediasi air dan tanah yang terkontaminasi uranium.
Profil Protein Klebsiella sp. dalam Kondisi Cekaman Osmotik dan Keasaman Ikhwan , Ali; Yuwono , Triwibowo; Widada , Jaka
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.969 KB) | DOI: 10.24002/biota.v16i2.94

Abstract

Penelitian dilakukan untuk mengetahui profil protein yang dibuat oleh Klebsiella sp. yang tumbuh dalam kondisi cekaman osmotik dan keasaman. Cekaman osmotik dilakukan menggunakan NaCl, sedangkan cekaman keasaman menggunakan aluminium sulfat. Klebsiella sp. ditumbuhkan dalam medium minimal yang ditambah dengan NaCl, atau aluminium sulfat, untuk menimbulkan efek cekaman tunggal, atau menggunakan kedua senyawa tersebut untuk menghasilkan efek cekaman ganda. Protein total yang diekstrak dari sel kemudian dielektroforesis pada SDS-PAGE 12%. Hasil analisis menunjukkan beberapa protein intraselular, protein membran, atau protein ekstraselular yang dibuat dalam kondisi cekaman spesifik. Dalam kondisi cekaman osmotik, dibuat protein intraselular berukuran 42,7 kDa, dan protein membran berukuran 53,3 kDa. Pada cekaman asam dihasilkan protein intraselular berukuran 54,7 kDa, 25,3 kDa, 14,2 kDa, dan satu protein membran berukuran 43,9 kDa, serta protein ekstraselular berukuran 17–29 kDa. Dalam kondisi cekaman ganda, terdeteksi satu protein intraselular spesifik berukuran 26,7 kDa dan satu protein membran berukuran 61,1 kDa. Dalam cekaman osmotik, diketahui terdapat korelasi positif, sedangkan dalam cekaman ganda terdapat korelasi negatif terhadap macam protein. Dalam cekaman keasaman, tidak diperoleh pola korelasi yang spesifik.
Effect of Coated Urea with Humic-Calcium on Transformation of Nitrogen in Coastal Sandy Soil: A Soil Column Method Sulakhudin, Sulakhudin; Syukur, Abdul; Shiddieq, Dja'far; Yuwono, Triwibowo
JOURNAL OF TROPICAL SOILS Vol 15, No 1: January 2010
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2010.v15i1.11-18

Abstract

Effect of Coated Urea with Humic-Calcium on Transformation of Nitrogen in Coastal Sandy Soil: A Soil Column Method (Sulakhudin, A  Syukur, D  Shiddieq and T  Yuwono):  In coastal sandy soil, mainly nitrogen losses due to leaching resulted to low fertilizer efficiency. Slow-release N fertilizers are proposed to minimize these losses, and humic-calcium coated urea has been examined. A soil column method was used to compare the effects of coated urea with humic-calcium on transformation and leaching loss of N in coastal sandy soil. The experiment aid to compare two kind source humic substances (cow manure and peat) which mixed with calcium as coated urea on transformation, vertical distribution and leaching N in coastal sandy soil. The concentration of humic-calcium coated urea i.e.1%, 5% and 10% based on their weight.  The results showed that urea coated with humic-calcium from cow manure (UCHM) and humic-calcium from peat (UCHP) increased the N total and available N in the soil and decreased leaching loss of N from the soil column. Compare to UCHP, UCHM in all concentration showed N-nitrate higher than N-ammonium on incubation length 2, 4 and 6 weeks. The N leached from a costal sandy soil with application coated urea with UCHM ranged from 21.18% to 23.72% of the total N added as fertilizer, for coated urea with UCHP they ranged between 21.44% and 23.25%, whereas for urea (control) reach 29.48%. Leaching losses of mineral N were lower when urea coated with UCHM compared to urea coated with UCHP or urea fertilizer.  The study concluded that the UCHM is better than UCHP in decreasing N leached from coastal sandy soil.
ISOLASI DAN IDENTIFIKASI MOLEKULAR BAKTERI TOLERAN URANIUM YANG BERPOTENSI DALAM BIOPRESIPITASI URANIUM Bernadetta Octavia; Triwibowo Yuwono; Agus Taftazani
GANENDRA Majalah IPTEK Nuklir Volume 21 Nomor 1 Januari 2018
Publisher : Website

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.982 KB) | DOI: 10.17146/gnd.2018.21.1.3909

Abstract

Penelitian mengenai bakteri toleran uranium yang berpotensi dalam biopresipitasi uranium telah dilakukan. Untuk menetapkan bakteri yang paling toleran uranium maka dilakukan skrining bertingkat dengan medium TGY cair yang mengandung larutan uranium dengan konsentrasi 0,4 mM; 0,8 mM; 1 mM; dan 2 mM terhadap isolat bakteri yang telah diisolasi dari limbah radioaktif cair aktivitas rendah fase organik dan fase air yang dimiliki oleh PSTA BATAN Yogyakarta. Dari hasil skrining awal (preliminary screening) ini diperoleh 51 isolat bakteri toleran uranium. Setelah dilakukan karakterisasi yang meliputi pengecatan Gram, pengamatan bentuk sel dan morfologi koloni serta penghitungan kinetika pertumbuhan dan dikombinasikan dengan teknik rep-PCR maka diperoleh 26 klaster dari 51 isolat bakteri pada tingkat keserupaan (similarity) 83%. Selanjutnya dari tiap klaster dipilih satu isolat dengan laju pertumbuhan spesifik (µ) tertinggi. Dengan demikian didapatkan 26 isolat bakteri toleran uranium yang   diuji selanjutnya sebagai bakteri polifosfat. Untuk mendapatkan bakteri polifosfat maka isolat bakteri ini ditumbuhkan dalam medium agar plate yang tidak mengandung P dan diinkubasikan selama 5 hari pada suhu ruang untuk diamati daya hidup (viability) dan perkembangannya. Berdasarkan hasil seleksi ini diperoleh 7 isolat bakteri polifosfat yang mampu tumbuh dan berkembang dengan menggunakan cadangan polifosfat di dalam selnya sebagai sumber energi dan berpotensi dalam biopresipitasi uranium.  Bakteri polifosfat ini diberi kode : A-14, A-25, A- 66, A-67 I, O-21, O-27 dan O-29. Selain karakterisasi dilakukan pula identifikasi molekular berdasarkan urutan nukleotida gen 16S rRNA terhadap 7 isolat bakteri polifosfat. Hasil analisis hubungan filogenetik 7 isolat bakteri polifosfat menggunakan bakteri acuan Bacillus cereus ATCC 14579 dan Acinetobacter radioresisten DSM 6976 dengan melihat nilai keserupaan dan perbedaan  nukleotida maka dapat ditetapkan bahwa isolat bakteri dengan kode A-35, A-14, A-66, O-27, O-21, dan O-29 memiliki kemiripan dengan Bacillus cereus sedangkan isolat bakteri dengan kode A-67 I memiliki kemiripan dengan Acinetobacter radioresistens.
An Active of Extracellular Cellulose Degrading Enzyme from Termite Bacterial Endosimbiont M. Saifur Rohman; Endang Pamulatsih; Yudi Kusnadi; Triwibowo Yuwono; Erni Martani
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.666 KB) | DOI: 10.22146/ijbiotech.15273

Abstract

Cellulase is an ezyme that specifically cleaves the 1,4-β-glycosidic bond of cellulose to produce thesmall fragments of simple carbohydrate. This work was aimed to characterize the extracellular cellulase fromPaenibacillus spp., which was previously isolated from macro termites, Odontotermes bhagwatii in our laboratory.Two Paenibacillus isolates were used in this experiment, namely Paenibacillus cellulositrophicus SBT1 andPaenibacillus, sp. SBT8. Analysis of the total proteins in the supernatants showed that P. cellulositrophicus SBT1and Paenibacillus sp. SBT8 roughly produced as much as 18.6 mg/l and 24.8 mg/l of extracellular cellulases,respectively. Enzymatic assay showed that SBT1 and SBT8 cellulase exhibited enzymatic acitivity of 0.17 U/mg and 0.12 U/mg, respectively. Temperature dependencies analysis indicated that both cellulases exhibitedmaximum activity at 35oC. At the temperature higher than 55oC, the enzymatic activities of both cellulases wereroughly 20% reduced compared to the maximum activity. SBT1 and SBT8 cellulases were both active at acidicpH. At basic pH (pH 8) the enzymatic activities of both cellulases were reduced roughly 30% compared to thatof acidic pH. Supplementing of Mg2+, Zn2+, and Ca2+ in range of 1-10 mM increased the enzymatic activity ofboth cellulases roughly 33 to 50%.
The Use Of Pulse Field GelElectrophoresis For Megaplasmid Isolatioan Of Pseudomonas solanacearum Siti Subandiyah; Triwibowo Yuwono; Wayan T. Artama
Jurnal Ilmu Pertanian Vol 5, No 4 (1994): September
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.60011

Abstract

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The Evaluation of Heat-Shock Promoter For Heterologous Gene Expression In Yeast Triwibowo Yuwono
Jurnal Ilmu Pertanian Vol 4, No 8 (1992): Februari
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.60051

Abstract

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SPECIES DETERMINATION OF GREEN ALGAE ISOLATED FROM JEPARA COASTAL REGION BASED ON MICROBIOLOGICAL, ECOPHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION FOR IMPROVEMENT OF CAROTENOID PRODUCTION Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini; Triwibowo Yuwono; Joedoro Soedarsono
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 1 (2006): Volume 10, Number 1, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.157 KB)

Abstract

A local isolate of green algae called C1 from Jepara waters is usually used as a source for carotenoid supplement for animal fisheries in the local area. This indigenous algae has been successfully purificated.  Although the local isolate was known as eucaryotic green algae Dunaliella, our previous molecular study by 18S rDNA analysis to determine the species of this organisms showed negative result. In order to improve carotenoid production especially detection of biosynthetic pathway from the organisms investigated in this study, the main purpose of this study was species determination of local isolate of green algae based on microbiologycal, ecophysiologycal and molecular characterization. The results of this research  indicated that local isolate of green algae posseses Cyanobacteria characteristic, especially Synechocystis. Analyses with 16S rDNA sequence from genome of green algae isolates were also in accordance with these results, showing close similarities with Synechocystis 16S rDNA sequence.  However, it should be noted that, instead of having Synechocystis dominant feature, it was also found that local isolate of green algae exhibit different characteristic in having chlorophyll a, chlorophyll b and lack of phycobillins. This character was typical for abberant Cyanophyta, Prochlorophyta.
The Effect of Various Salinity Level on the Growth and Characterization of Dunaliella sp Isolated from Jepara Waters Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini; Triwibowo Yuwono; Joedoro Sudarsono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 3 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.064 KB) | DOI: 10.14710/ik.ijms.9.3.136-140

Abstract

Dunaliella adalah salah satu biota dengan kandungan β-carotene cukup tinggi. Upaya optimalisasi produksi bcarotene pada Dunaliella berhadapan dengan beberapa masalah kultivasi, untuk mendapatkan species yang paling potensial. Hal ini terkait dengan keterbatasan pengetahuan karakteritik ecophysiologi. Alga hijau Dunaliella diketahui dapat tumbuh pada media dengan kandungan garam yang cukup tinggi, namun karena pemahaman characteristik yang keliru dapat menyebabkan identifikasi yang salah pada satu species dalam genus Dunaliella. Kultur laboratoris pada media microcosms berdasarkan salinitas telah dilakukan untuk mengetahui tingkat pertumbuhan dan karakterisasi Dunaliella sp. dari perairan Jepara. Hasil pengamatan menunjukkan bahwa Dunaliella sp. dapat beradaptasi pada salinitas 0 sampai dengan 30 ‰. Berdasarkan kepada perubahan warna pigmen Dunaliella sp. yang tidak menjadi merah pada media pemeliharaan sampai dengan 25 ‰, maka jenis yang dijumpai di Jepara mempunyai karakter dan secara taksonomis berafiliasi dengan Dunaliella viridis.Kata kunci: Dunaliella sp, Salinity, Growth, CharacterizationDunaliella is one the most enriched β-carotene eucaryotic organism known. The attempt to optimize bcarotene production from Dunaliella has faced with several problems related to its growth management,which was suspectedly unable to meet the needs of the cultured species. This is primarily because the ecophysiological characteristic affecting growth of Dunaliella have not been sufficiently understood. It wasknown that the halophilic species of the green alga Dunaliella was grown in concentrated salt solutions, but based on this characterization, some misnamed of species in genus Dunaliella also have arisen due to wrongcharacterization understanding. Laboratory cultures and mixed-species microcosms were used to asses the growth and characterization of Dunaliella sp. from Jepara Coastal Region with special emphasis on the several factors that affecting growth of organisms including salinity. The result showed that Dunaliella sp. could adapted to a variety of salt concentration from as low as 0.0 % to salt saturation of about 30 ‰. Based on its pigment colour that Dunaliella sp. doesn’t turn red in the growth on salinities up to 25 ‰, it can be characterized and affiliated taxonomically as Dunaliella viridis.Key words: Dunaliella sp, Salinity, Growth, Characterization
Co-Authors Abdul Syukur Agus Taftazani Agus Taftazani Ali Ikhwan Arofatullah, Nur Akbar Bernadetta Octavia Diah Rachmawati Donny Widianto Endang Kusdiyantini Endang Kusdiyantini Endang Pamulatsih Erni Martani HARI HARTIKO Hasna Dyah Kusumardani Hermin Pancasakti Kusumaningrum Hermin Pancasakti Kusumaningrum Ikhwan , Ali Irfan Dwidya Prijambada JAKA WIDADA JOEDORO SOEDARSONO JOEDORO SOEDARSONO Joedoro Sudarsono Kasumbogo Untung M. Saifur Rohman Mizu lstianto Shiddieq, Dja'far Siti Subandiyah Sulakhudin Sulakhudin Supriyadi Supriyadi WAHYU IRAWATI Wayan T. Artama Widada , Jaka Y. Andi Trisyono Yudi Kusnadi